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Comet Assay of DNA Damage 15720. Collins, A., Dusinska, M., Franklin, M., Somorovska, M., Petrovska, H., Duthie,S., Fillion, L., Panayiotidis, M., Raslova, K., and Vaughan, N. (1997) Comet assayin human biomonitoring studies: reliability, validation, and applications. Env. Molec.Mutagenesis 30, 139–146.21. Green, M. H. L., Lowe, J. E., Delaney, C. A., and Green, I. C. (1996) Comet assayto detect nitric oxide-dependent DNA damage in mammalian cells. MethodsEnzymol. 269, 243–266.22. Thomas, S., Green, M. H. L., Lowe, J. E., and Green, I. C. (1997) Measurement ofDNA damage using the Comet assay (Chapter 29), in Nitric Oxide Protocols(Titheradge, M., ed.), Humana Press, Totowa, NJ, pp. 301–309.23. Collins, A. R., Dobson, V. L., Dusinska, M., Kennedy, G., and Stetina, R. (1997)The comet assay: what can it really tell us? Mutat. Res. 375, 183–193.24. Woollons, A., Clingen, P. H., Price, M. L., Arlett, C. F., and Green, M. H. L. (1997)Induction of mutagenic DNA damage in human fibroblasts after exposure to artificialtanning lamps. Br. J. Dermatol. 137, 687–692.25. Boiteux, S., O’Connor, T. R., and Laval, J. (1987) Formamidopyrimidine-DNAglycosylase of Escherichia coli: cloning and sequencing of the fpg structural geneand overproduction of the protein. EMBO J. 6, 3177–3183.26. Dusinska, M. and Collins, A. (1996) Detection of oxidized purines and UV-inducedphotoproducts in DNA of single cells by inclusion of lesion-specific enzymes in thecomet assay. ATLA 24, 405–411.27. Duthie, S. J. and McMillan, P. (1997) Uracil misincorporation in human DNAdetected using single cell gel electrophoresis. Carcinogenesis 18, 1709–1714.28. Cole, J. and Arlett, C. F. (1984) The detection of gene mutations in cultured mammaliancells, in Mutagenicity Testing — A Practical Approach (Titheradge, M.,Venitt,S., and Parry, J. M., eds.), IRL Press, Oxford, pp. 233–273.29. Klaude, M., Eriksson, S., Nygren, J., and Ahnstrom, G. (1996) The Comet assay—mechanisms and technical considerations. Mutat. Res. 363, 89–96.30. Singh, N. P., Graham, M. M., Singh, V., and Khan A. (1995) Induction of DNAsingle-strand breaks in human-lymphocytes by low-doses of γ-rays. Int. J. Radiat.Biol. 68, 563–569.31. Collins, A. R., Fleming, I. A., and Gedik, C. M. (1994) In vitro repair of oxidativeand ultraviolet-induced DNA damage in supercoiled nucleoid DNA by human cellextract. Biochim. Biophys. Acta 1219, 724–727.32. Green, M. H. L., Waugh, A. P. W., Lowe, J. E., Harcourt, S. A., Cole, J., and Arlett,C. F. (1994) Effect of deoxyribonucleosides on the hypersensitivity of human peripheralblood lymphocytes to UV-B and UV-C irradiation. Mutat. Res. 315, 25–32.

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