Aging Aging

Immunogenetics and Life-Span 333Table 5Probes Used for HLA-B TypingWashSequence temp Picomoles NucleotideProbe 5' → 3' (°C) used positionExon 231 (B89) GGTATTTCGACACCGCC 56 40 17–3332 (B156) GGACGGCACCCAGTT 52 40 84–9833 (B168) GTTCGTGCGGTTCGA 50 40 96–11009 (BL09) GAGTCCGAGAGAGGAGCC 57 6 123–14001 (BL01) GAGGAAGGAGCCGCGGGC 64 20 129–14602 (BL02) GAGGACGGAGCCCCGGGC 64 40 129–14607 (BL07) GAGGATGGCGCCCCGGGC 64 60 129–14634 (B249) TTGGGACGGGGAGAC 50 40 177–19124 (BL24) GGGAGACACAGATCTCCA 55 40 185–20205 (BL05) ACACAGATCTTCAAGACC 55 14 190–20710 (BL10) GATCTACAAGGCCCAGGC 58 10 195–21212 (BL12) ATCTGCAAGGCCAAGGCA 56 20 196–21318 (BL18) ACTGACCGAGTGAGCCTG 58 20 217–23435 (B73) ACTGACCGAGTGGGCCTG 63 40 217–23420 (BL20)* AGCGGAGCGCGGTGCGCA 64 40 233–25021 (BL21) CGGAACCTGCGCGGCTAC 62 40 235–25222 (BL22) CGGACCCTGCTCCGCTAC 61 30 235–25223 (BL23) CGGATCGCGCTCCGCTAC 62 40 235–252Exon 327 (BL27) CTCACACTTGGCAGAGGA 56 20 5–2236 (B348) TCACACCATCCAGAGG 49 50 6–2137 (B354) CATCCAGGTGATGTAT 46 40 12–2728 (BL28) CCAGTGGATGTATGGCTG 56 40 15–3238 (B361) AGGATGTTTGGCTGC 48 40 19–3326 (BL26) CTGCGACCTGGGGCCCGA 65 40 30–4730 (BL30) GGCATAACCAGTTAGCCT 54 50 65–8239 (B409) TATGACCAGGACGCCT 55 40 67–8240 (B427) GACGGCAAAGATTACA 46 40 85–10041 (B499) ACCCAGCTCAAGTGG 47 40 157–17142 (B505) CGCAAGTTGGAGGC 46 40 163–17643 (B532) GAGCAGCTGAGAGCCT 52 40 190–20544 (B539) GAGAACCTACCTGGA 46 40 197–21145 (B553a) GAGGGCCTGTGCGT 48 40 211–22446 (B553b) GAGGGCACGTGCGT 48 40 211–22447 (B566) TGGAGTCGCTCCGC 48 40 224–23748 (B597) GAAGGACACGCTGGA 52 40 255–26949 (B599) AGGACAAGCTGGAGCG 52 40 257–272aComplementary to coding sequence.