Aging Aging

Immunogenetics and Life-Span 347Table 13HLA-DR 3/11/6 Subgroup SSOP Patterns1 2 3 4 5 6 7 8 9 10 11DR3 3.1 + + +3.2 + +3.3 + +0308 + + +DR11 11.1 +11.2 + +11.3 + +1107 + +1117 + +1126 + +DR13 13.1 + +13.2/1424 +1303 +1304 +1310 + +1313 +1318/14.3 + +1327 + + +13.4DR14 14.1 + +14.2 + +14.4 +14.5 + +14.6 + +14.7 +1413 +1416 + +(continued)in °C to 2× (number of A + T bases) +4 × (number of G + C bases). Thereafter weadjust the wash temperature by 1°C either up or down according to the probereaction at the melting temperature. We also start with 20 pmol of probe andadjust accordingly.9. When the probe conditions, that is, number of picomoles and wash temperaturehave been determined it is worthwhile to keep a record on the performance of theprobes, that is, whether the probe is not giving an adequate signal with its positivecontrol or whether it is crossreacting with controls with which it should benegative. On occasions the conditions for the probes need to be altered. This in away is similar to HLA sera whereby after long-term storage the specificities iden-