Aging Aging

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60 Pawelection, the VERUM Foundation, the Novartis Foundation for GerontologicalResearch, the ƒortüne Program of University of Tübingen Medical Faculty,and the European Commission (see http://www.medizin.uni-tuebingen.de/eucambis/).References1. Bach, F. H., Inouye, H., Hank, J. A., and Alter, B. J. (1979) Human T-lymphocyteclones reactive in primed lymphocyte typing and cytotoxicity. Nature 281,307–309.2. Pawelec, G. and Wernet, P. (1980) Restimulation properties of human alloreactivecloned T cell lines. Dissection of HLA-D-region alleles in population studies and infamily segregation analysis. Immunogenetics 11, 507–519.3. Morgan, D. A., Ruscetti, F. W., and Gallo, R. C. (1976) Selective in vitro growth ofT-lymphocytes from normal human bone marrows. Science 193, 1007–1008.4. Effros, R. B. and Walford, R. L. (1984) T cell cultures and the Hayflick limit. Hum.Immunol. 9, 49–65.5. Pawelec, G. (1985) Functions and changing activities of interleukin 2-dependenthuman T lymphocyte clones derived from sensitization in mixed leukocyte cultures,in T Cell Clones (von Boehmer, H. and Haas, W., eds.), Elsevier, Amsterdam,Holland, pp. 311–322.6. Johnson, J. P., Cianfriglia, M., Glasebrook, A. L. and Nabholz, M. (1982) Karyotypeevolution of cytolytic T cell lines, in Isolation, Characterisation, and Utilisationof T Lymphocyte Clones (Fathman, C. G., Fitch, F. W., eds.), Academic Press, NewYork, pp. 183–191.7. Kaltoft, K., Pedersen, C. B., Hansen, B. H., and Thestrup-Pedersen, K. (1995)Appearance of isochromosome 18q can be associated with in vitro immortalizationof human T lymphocytes. Cancer Genet. Cytogenet. 81, 13–16.8. Kahle, P., Wernet, P., Rehbein, A., Kumbier, I., and Pawelec, G. (1981) Cloning offunctional human T lymphocytes by limiting dilution: impact of feeder cells andinterleukin 2 sources on cloning efficiencies. Scand. J. Immunol. 4, 493–502.9. Pawelec, G., Schwuléra, U., Blaurock, M., Busch, F. W., Rehbein, A., Balko, I., andWernet, P. (1987) Relative cloning efficiencies and long-term propagation capacityfor T cell clones of highly purified natural interleukin 2 compared to recombinantinterleukin 2 in man. Immunobiology 174, 67–75.10. Effros, R. B. and Pawelec, G. (1997) Replicative senescence of T lymphocytes:does the Hayflick Limit lead to immune exhaustion? Immunol. Today 18, 450–454.11. Pohla, H., Adibzadeh, M., Buhring, H. J., Siegels-Hubenthal, P., Deikeler, T.,Owsianowsky, M., Schenk, A., Rehbein, A., Schlotz, E., Schaudt, K., and Pawelec,G. (1993) Evolution of a CD3 + CD4 + alpha/beta T-cell receptor + mature T-cell clonefrom CD3 - CD7 + sorted human bone marrow cells. Dev. Immunol. 3, 197–210.12. Preffer, F. I., Kim, C. W., Fischer, K. H., Sabga, E. M., Kradin, R. L., and Colvin, R.B. (1989) Identification of pre-T cells in human blood. Extrathymic differentiationof CD7 + CD3 - cells into CD3 + gamma/delta or alpha/beta + T cells. J. Exp. Med.170, 177–190.
Human T-Cell Clones 6113. Adibzadeh, M., Pohla, H., Rehbein, A., and Pawelec, G. (1995) Long-term cultureof monoclonal human T lymphocytes: models for immunosenescence? Mech. Ageing.Dev. 83, 171–183.14. Pawelec, G., Rehbein, A., Haehnel, K., Merl, A., and Adibzadeh, M. (1997) HumanT cell clones as a model for immunosenescence. Immunol. Rev. 160, 31–43.15. Freedman, A. R., Zhu, H. H., Levine, J. D., Kalams, S., and Scadden, D. T. (1996)Generation of human T lymphocytes from bone marrow CD34 + cells in vitro. Nat.Med. 2, 46–51.
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Page 52 and 53: 54 Pawelecdiluted cells on an irrad
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114 Engel, Adibzadeh, and Pawelecha
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Xenobiotic-Metabolizing Enzymes 119
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Assessing Antioxidant Status 1339As
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Assessing Antioxidant Status 135Fig
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Assessing Antioxidant Status 137Tab
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Assessing Antioxidant Status 139add
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Assessing Antioxidant Status 14121.
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Comet Assay of DNA Damage 14310Meas
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Comet Assay of DNA Damage 145contro
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Comet Assay of DNA Damage 1476. Lys
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Comet Assay of DNA Damage 1494. Gen
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Comet Assay of DNA Damage 151anode
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Comet Assay of DNA Damage 1535. As
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Comet Assay of DNA Damage 15515. In
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Comet Assay of DNA Damage 15720. Co
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160 van der Schanswithin 1 h, after
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162 van der Schans10. High-binding
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164 van der Schans3. Neutralize aft
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166 van der SchansThe calculations
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8-oxoguanine Levels in Nuclear DNA
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Mutation and the Aging Process 1791
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Mutation and the Aging Process 181m
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Mutation and the Aging Process 183b
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190 HouThe HPRT mutational spectrum
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192 Houamplifications are carried o
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194 Hou4. Reading: Load 10 µL of t
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196 Hou4.2. MP-PCRUse preferably DN
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Susceptibility of LDL to Oxidation
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Analysis of Pentosidine 20916Measur
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Analysis of Pentosidine 211Fig. 2.
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Analysis of Pentosidine 213Fig. 4.
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Analysis of Pentosidine 2152. Preci
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Analysis of Pentosidine 2177. Nagar
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222 Miquellipoperoxides and malonal
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224 Miquelat 4°C. Protect this sol
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226 Miquel3.1. Animal AnesthesiaMed
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228 Miquelsteps to carry out a conv
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230 Miquelsuspending it over the so
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232 Miquelcontrast, washing with bu
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234 MiquelFig. 3. Electron microsco
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Damage to Mitochondria 23718Causes
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Damage to Mitochondria 239Fig. 1. E
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Damage to Mitochondria 2412. To obt
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Damage to Mitochondria 243synthesis
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Mitochondrial DNA Mutations 24519An
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Mitochondrial DNA Mutations 247F.P.
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Mitochondrial DNA Mutations 249desi
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Mitochondrial DNA Mutations 2514. M
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Mitochondrial DNA Mutations 253Fig.
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Mitochondrial DNA Mutations 25568°
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Mitochondrial DNA Mutations 257Fig.
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Mitochondrial DNA Mutations 2593. P
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Mitochondrial DNA Mutations 261X-10
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Mitochondrial DNA Mutations 26315.
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Mitochondrial DNA Mutations 26520An
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Mitochondrial DNA Mutations 267Tabl
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Mitochondrial DNA Mutations 269muta
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Mitochondrial DNA Mutations 2717. P
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Mitochondrial DNA Mutations 2733.2.
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Mitochondrial DNA Mutations 275that
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Mitochondrial DNA Mutations 27711.
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282 BeckmanWhatever the mechanism e
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284 BeckmanT-cell adherence to vasc
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286 BeckmanT3 hybridoma cells satis
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288 BeckmanHaving identified a cand
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292 Grubeck-Loebenstein, Saurwein-T
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NK Cell Function in Aging 31123Age-
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NK Cell Function in Aging 3135. Pet
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NK Cell Function in Aging 31518. Io
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NK Cell Function in Aging 3173.3. C
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NK Cell Function in Aging 3199mL of
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Immunogenetics and Life-Span 32124I
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Immunogenetics and Life-Span 323Tab
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Immunogenetics and Life-Span 325rea
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Immunogenetics and Life-Span 3273.
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Immunogenetics and Life-Span 3313.5
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Immunogenetics and Life-Span 349to
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354 Yuto-implement intervention and
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356 Yuof their quality control proc
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358 Yugenesis of major diseases (1-
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Genetically Engineered Mice 36126Th
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Genetically Engineered Mice 363as t
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Genetically Engineered Mice 365in b
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Genetically Engineered Mice 367numb
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Genetically Engineered Mice 369mula
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