Aging Aging

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8-oxoguanine Levels in Nuclear DNA 177Fig. 2. ECD and UV traces for rat liver nuclear DNA with a typically low backgroundlevel of 8-oxo-dG.and 8-oxo-dG have an absorbance of 13AU (254 nm) and 12.3AU (245 nm),respectively. Standard curves are run on each day, with dG at 50, 100, and 150 mMand 8-oxo-dG at 0.25, 0.5, 1, and 2 nM.4. Do not leave samples for longer than 12 h at 5°C in the autosampler; there issome evidence of oxidation after this time.5. Check for carryover of 8-oxo-dG by inserting a 0.25 nM 8-oxo-dG standard aftereach set of quadruplicate samples. Check that the peak area is constant throughoutthe run.6. The concentration of dG is estimated from the UV peak; 8-oxo-dG from the electrochemicalsignal at 400 mV. Results are usually expressed as the number ofresidues of 8-oxo-dG per 10 5 dG.4. Notes1. As DF is light sensitive, cover tubes with foil, keep lid on water bath, etc. AlsoDF is unstable at room temperature and 4°C, so the solid and buffers containingDF should be stored at –20°C. The buffers should be freshly thawed before useand surplus discarded.2. Do not attempt to economize by refreezing working solutions of enzymes andsaving them for use at a later date. Be ruthless and discard unused solutions.AcknowledgmentsWe are grateful for financial support from the Ministry of Agriculture, Fisheriesand Food and the Scottish Office Agriculture, Environment and FisheriesDepartment.
178 Wood et al.References1. Collins, A., Cadet, J., Epe, B., and Gedik, C. (1997) Problems in the measurementof 8-oxoguanine in human DNA. Carcinogenesis 18, 1833–1386.2. Gedik, C. M., Wood, S. G., and Collins, A. R. (1998) Measuring oxidative damageto DNA; HPLC and the comet assay compared. Free Radic. Res., 29, 609–615.3. Richter, C., Park, J.-W., and Ames, B. N. (1988) Normal oxidative damage tomitochondrial and nuclear DNA is extensive. Proc. Natl. Acad. Sci. USA 85,6465–6467.
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Understanding Aging 9the intestine
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Understanding Aging 117. Do long-li
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Detection of Molecular Events 715De
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Detection of Molecular Events 75Fig
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Detection of Molecular Events 772.
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Detection of Molecular Events 79Fig
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86 Kirk and Millerexperiments on un
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Xenobiotic-Metabolizing Enzymes 119
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Page 134 and 135: Comet Assay of DNA Damage 14310Meas
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Page 158 and 159: 8-oxoguanine Levels in Nuclear DNA
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Page 184 and 185: Susceptibility of LDL to Oxidation
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Page 196 and 197: Analysis of Pentosidine 211Fig. 2.
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Page 204 and 205: 222 Miquellipoperoxides and malonal
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234 MiquelFig. 3. Electron microsco
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Damage to Mitochondria 23718Causes
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Damage to Mitochondria 239Fig. 1. E
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Damage to Mitochondria 2412. To obt
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Damage to Mitochondria 243synthesis
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Mitochondrial DNA Mutations 24519An
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Mitochondrial DNA Mutations 247F.P.
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Mitochondrial DNA Mutations 249desi
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Mitochondrial DNA Mutations 2514. M
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Mitochondrial DNA Mutations 253Fig.
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Mitochondrial DNA Mutations 25568°
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Mitochondrial DNA Mutations 257Fig.
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Mitochondrial DNA Mutations 2593. P
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Mitochondrial DNA Mutations 261X-10
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Mitochondrial DNA Mutations 26315.
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Mitochondrial DNA Mutations 26520An
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Mitochondrial DNA Mutations 267Tabl
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Mitochondrial DNA Mutations 269muta
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NK Cell Function in Aging 3173.3. C
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NK Cell Function in Aging 3199mL of
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Immunogenetics and Life-Span 32124I
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Immunogenetics and Life-Span 349to
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Genetically Engineered Mice 36126Th
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Aging Aging

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