Aging Aging

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Immunogenetics and Life-Span 343Table 11HLA-C SSOP PatternsProbes 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23HLA-Cw01 + + +02021 + + + + +02022/024 + + + + + +02023 + + + + +0302 + + + +0303 + + + +0304 + + +04 + + +0501 + + +0602 + + + + +7.1 a + + + + +0702 + + + + + +0703 + + + + +0704 + + + + +0707 + + + + +0801/03 + + + +0802 + + +1202 + + + + + +1203 + + + + +1204 + + + +1301 + + + + + + +14 + + + +1502/03 + + +1504 + + +1505 + +1601 + + + + +1602 + + + + +17 + + + + +18 + + + +The alleles listed are those identified in HLA-C sequences from ASHI, April 1997.a 7.1 represents 0701, 0705, 0706.to poor amplification. Interpretation of weak hybridization signals can give anincorrect result.5. When determining the conditions necessary for this technique one importantaspect is to ensure that the amplification works for both alleles. On some occasionswhen we were determining the amplification conditions we found that aproduct could be obtained whereby only one allele could be detected. Therefore,
344 Middleton, Curran, and WilliamsTable 12HLA-DR SSOP PatternsProbe 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 22 23 24 25 26HLA-DR allele1 1.1 + + +01022 + +0103 + + +2 15.2 + + +1503/1607 + +16.1 + + +1604 + + + +1608 + + + +3 3.1 + + + +3.2 + + +0304 + + +0308 + + + + +4 4.2 + + + + + +4.3 + + + + + +4.5 + + + + +4.6 + + + + + +4.7 + + + + + +4.8 + + + + + + +0409 + + + + + + +0412 + + + + + + +0415 + + + + + +0418 + + + + + +0422 + + + + + +11 11.2 + + + + +11.4 + + + +11.16 + + + + + +1105/30 + + +1107 + + + + +1109 + + + + +1117 + + + + +1122 + + + +1123/25 + + + + +1126 + + + + +12 12.1 + + +1204 + + + +1205 + +(continued)
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6 Strehlerlead to possible death si
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Understanding Aging 9the intestine
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Understanding Aging 117. Do long-li
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Understanding Aging 13is, the great
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Understanding Aging 15While this re
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Understanding Aging 172. Pearl, R.
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Understanding Aging 1945. Perovic,
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24 Cristofalo, Volker, and Allenmen
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26 Cristofalo, Volker, and Allenrec
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28 Cristofalo, Volker, and Allenski
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30 Cristofalo, Volker, and AllenTab
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34 Cristofalo, Volker, and AllenNa
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38 Cristofalo, Volker, and Allentak
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40 Cristofalo, Volker, and Allenb.
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42 Cristofalo, Volker, and Allenand
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44 Cristofalo, Volker, and AllenRef
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46 Cristofalo, Volker, and Allen33.
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48 Cristofalo, Volker, and Allen61.
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50 Cristofalo, Volker, and Allenhum
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52 Cristofalo, Volker, and Allen125
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54 Pawelecdiluted cells on an irrad
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56 Pawelec3. Method3.1. Source of C
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58 Pawelecweekly or fortnightly sub
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60 Pawelection, the VERUM Foundatio
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Telomeres and Replicative Senescenc
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Detection of Molecular Events 715De
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Detection of Molecular Events 73hav
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Detection of Molecular Events 75Fig
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Detection of Molecular Events 772.
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Detection of Molecular Events 79Fig
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86 Kirk and Millerexperiments on un
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88 Kirk and MillerFig. 1. (A) Analy
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90 Kirk and MillerFurthermore, limi
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92 Kirk and Millersufficiently dilu
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94 Kirk and Miller4. Run samples on
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96 Kirk and Miller16. Lange-Carter,
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98 Engel, Adibzadeh, and PawelecPCR
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100 Engel, Adibzadeh, and Pawelec5.
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102 Engel, Adibzadeh, and PawelecTa
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104 Engel, Adibzadeh, and PawelecTa
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106 Engel, Adibzadeh, and Pawelecam
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110 Engel, Adibzadeh, and Pawelecfo
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114 Engel, Adibzadeh, and Pawelecha
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Xenobiotic-Metabolizing Enzymes 119
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Assessing Antioxidant Status 1339As
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Assessing Antioxidant Status 135Fig
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Assessing Antioxidant Status 137Tab
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Assessing Antioxidant Status 139add
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Assessing Antioxidant Status 14121.
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Comet Assay of DNA Damage 14310Meas
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Comet Assay of DNA Damage 145contro
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Comet Assay of DNA Damage 1476. Lys
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Comet Assay of DNA Damage 1494. Gen
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Comet Assay of DNA Damage 151anode
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Comet Assay of DNA Damage 1535. As
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Comet Assay of DNA Damage 15515. In
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Comet Assay of DNA Damage 15720. Co
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160 van der Schanswithin 1 h, after
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162 van der Schans10. High-binding
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164 van der Schans3. Neutralize aft
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166 van der SchansThe calculations
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8-oxoguanine Levels in Nuclear DNA
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Mutation and the Aging Process 1791
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Mutation and the Aging Process 181m
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Mutation and the Aging Process 183b
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190 HouThe HPRT mutational spectrum
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192 Houamplifications are carried o
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194 Hou4. Reading: Load 10 µL of t
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196 Hou4.2. MP-PCRUse preferably DN
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Susceptibility of LDL to Oxidation
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Analysis of Pentosidine 20916Measur
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Analysis of Pentosidine 211Fig. 2.
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Analysis of Pentosidine 213Fig. 4.
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Analysis of Pentosidine 2152. Preci
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Analysis of Pentosidine 2177. Nagar
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222 Miquellipoperoxides and malonal
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224 Miquelat 4°C. Protect this sol
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226 Miquel3.1. Animal AnesthesiaMed
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228 Miquelsteps to carry out a conv
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230 Miquelsuspending it over the so
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232 Miquelcontrast, washing with bu
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234 MiquelFig. 3. Electron microsco
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Damage to Mitochondria 23718Causes
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Damage to Mitochondria 239Fig. 1. E
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Damage to Mitochondria 2412. To obt
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Damage to Mitochondria 243synthesis
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Mitochondrial DNA Mutations 24519An
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Mitochondrial DNA Mutations 247F.P.
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Mitochondrial DNA Mutations 249desi
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Mitochondrial DNA Mutations 2514. M
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Mitochondrial DNA Mutations 253Fig.
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Mitochondrial DNA Mutations 25568°
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Mitochondrial DNA Mutations 257Fig.
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Mitochondrial DNA Mutations 2593. P
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Mitochondrial DNA Mutations 261X-10
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Mitochondrial DNA Mutations 26315.
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Mitochondrial DNA Mutations 26520An
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Mitochondrial DNA Mutations 267Tabl
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Mitochondrial DNA Mutations 269muta
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Mitochondrial DNA Mutations 2717. P
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Mitochondrial DNA Mutations 2733.2.
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Mitochondrial DNA Mutations 275that
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Mitochondrial DNA Mutations 27711.
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282 BeckmanWhatever the mechanism e
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284 BeckmanT-cell adherence to vasc
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286 BeckmanT3 hybridoma cells satis
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288 BeckmanHaving identified a cand
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290 Beckmanthe plates vigorously wi
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Page 288 and 289: NK Cell Function in Aging 31123Age-
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Page 294 and 295: NK Cell Function in Aging 3173.3. C
Page 296 and 297: NK Cell Function in Aging 3199mL of
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Page 308 and 309: Immunogenetics and Life-Span 3313.5
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Page 328 and 329: 354 Yuto-implement intervention and
Page 330 and 331: 356 Yuof their quality control proc
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Page 334 and 335: Genetically Engineered Mice 36126Th
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Page 338 and 339: Genetically Engineered Mice 365in b
Page 340 and 341: Genetically Engineered Mice 367numb
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Page 344 and 345: Genetically Engineered Mice 371Othe
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Aging Aging

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