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LUCRĂRI ŞTIINŢIFICE Vol. 54 NR. 2 SERIA HORTICULTURĂ

LUCRĂRI ŞTIINŢIFICE Vol. 54 NR. 2 SERIA HORTICULTURĂ

LUCRĂRI ŞTIINŢIFICE Vol. 54 NR. 2 SERIA HORTICULTURĂ

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formazan/g, a mixture of micronutrients - 0.0247 μg formazan/g and that containing the<br />

copper - 0.1633 μg formazan/g, suggesting that, the α-ketoglutarate -dehydrogenase in<br />

Monilinia laxa species is inhibited by copper but that its combination with other trace<br />

elements enhances this inhibitory effect.<br />

μg formazan/g<br />

3<br />

2,5<br />

2<br />

1,5<br />

V1- Bor<br />

V2- Cupru<br />

V3- Mangan<br />

V4- Molibden<br />

V5- Fier<br />

V6- Zinc<br />

V7- Mixtură<br />

V8- Martor<br />

1<br />

0,5<br />

0<br />

7 days<br />

14 days<br />

Fig. 1 - The influence of the trace elements<br />

on the isocitrate-dehydrogenase in<br />

Monilinia laxa<br />

The aging of the mycelial culture has induced a decrease of the succinatedehydrogenase<br />

activity in the control sample- 0.3186 μg formazan/g. Has been<br />

118<br />

μg formazan/g<br />

2,5<br />

2<br />

1,5<br />

1<br />

V1- Bor<br />

V2- Cupru<br />

V3- Mangan<br />

V4- Molibden<br />

V5- Fier<br />

V6- Zinc<br />

V7- Mixtură<br />

0,5<br />

0<br />

V8- Martor<br />

7 days<br />

Fig.2 - The influence of the trace<br />

elements on the α-ketoglutaratedehydrogenase<br />

in Monilinia laxa<br />

After 14 days of incubation, the α-ketoglutarate-dehydrogenase activity value<br />

in the control sample was 1.0481 μg formazan/g. The simultaneity of action of the<br />

trace elements mixture - induced as α-ketoglutarate dehydrogenase a strong increase<br />

in contrast with other medium supplemented with trace elements - 1.6872 μg<br />

formazan/g. All other trace elements have caused a significant inhibition of this<br />

biochemical parameters compared with the control sample, copper, iron, zinc, boron<br />

molybdenum giving relatively uniform values, while manganese, compared with<br />

them, was comparatively more tolerant - 0.5292 μg formazan/g.<br />

The results of the time-course monitoring of the succinate-dehydrogenase<br />

activity in Monilinia laxa mycelium cultivated on medium enriched with the various<br />

trace elements are reproduced graphically in figure 3. As a first observation, it appears<br />

that, at 7 days after inoculation of the culture, all medium variants have as this<br />

enzyme activity the values were lower than the control sample, who had a value of<br />

1.2590 μg formazan/g.<br />

μg formazan/g<br />

1,4<br />

1,2<br />

1<br />

0,8<br />

0,6<br />

0,4<br />

0,2<br />

0<br />

V1- Boron<br />

V2-Copper<br />

V3-Manganese<br />

V4- Molybdenum<br />

V5- Iron<br />

V6- Zinc<br />

V7- Mixture<br />

V8- Control<br />

7 days<br />

14 days<br />

Fig. 3 - The influence of the trace elements<br />

on the succinate- dehydrogenase in<br />

Monilinia laxa<br />

μg formazan/g<br />

1,8<br />

1,6<br />

1,4<br />

1,2<br />

1<br />

0,8<br />

0,6<br />

0,4<br />

0,2<br />

0<br />

V1- Boron<br />

V2-Copper<br />

V3-Manganese<br />

V4- Molybdenum<br />

V5- Iron<br />

V6- Zinc<br />

V7- Mixture<br />

V8- Control<br />

14 days<br />

7 days<br />

14 days<br />

Fig. 4 - The influence of the trace<br />

elements on the malate- dehydrogenase<br />

in Monilinia laxa

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