LUCRĂRI ŞTIINŢIFICE Vol. 54 NR. 2 SERIA HORTICULTURĂ

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CONCLUSIONS 1. The value of the mitotic index was 15,6 which denotes that the plant was in a phase of active growth. 2. The results related with the repartition of cells per each phases of division showed that most of the cells are in prophase (36,39%), followed by metaphase (26,64%), anaphase (21,11%) and telophase (15,84%). 3. The chromosomal aberration observed in the ana-telophases of cells was mainly ana-telophases with simple or multiple bridges and ana-telophases with fragments, but also expelled or late chromosomes and multipolar ana-telophases. All the aberattion observed could be classified as: aberrations that affect the chromosome, aberrations that affect the chromatid, mixed or others. But we also observed metaphases with lagging chromosomes, expelled chromosomes or ring chromosomes, multipolar ana-telophases, as well as binucleate cells and interphases with micro-nucleuses. Acknowledgments: This work was financially supported by PN II 52- 174/2008. REFERENCES 1. Harbage James F., 2001 - Micropropagation of Allium ursinum, E. pallida, and E. purpurea from stem and seed explants. HortScience. 36(2): 360-364. 2. Hassell R.L., R. Dufault, T. Phillips, 2004 - Relationship among seed size, source and temperature on germination of Allium ursinum, pallida, and purpurea. Acta Horticulturae. 3. Li M. and M. Zhang, 1991 - Technology for plant chromosome research. Northwest Forest Univ. Press, Shenyiang, China. p. 31–39. 84
STIMULATION OF STILBENE POLYPHENOL BIOSYNTHESIS, UNDER IN VIVO CONTROLLED CONDITIONS, IN SOME GRAPE VARIETIES FOR RED AND WHITE QUALITY WINE STIMULAREA BIOSINTEZEI POLIFENOLILOR STILBENICI ÎN CONDIŢII CONTROLATE IN VIVO LA UNELE SOIURI DE STRUGURI PENTRU VINURI ROŞII ŞI ALBE DE CALITATE 85 BEJAN Carmen 1 , VIŞOIU Emilia 1 e-mail: cabej2003@yahoo.fr Abstract. It is known that, in response to stress (physical or chemical elicitors, a parasitic infection), the vine can synthesize natural molecules, generally called phytoalexins, enabling it to adapt to this stress. Stilbene polyphenols are the major constituents of these molecules, with the resveratrol (trans-3, 5, 4-trihydroxystilbene), as the major one. Among the chemical agents able to induce the synthesis of resveratrol in the grape-vine plants they come in contact with, the aluminium chloride is the most effective. The undertaken study aimed to determine the active usable doses of AlCl3, able to stimulate the biosynthesis of polyphenols and implicitly of resveratrol in the vine plants, under in vivo controlled conditions. Several Vitis vinifera L. genotypes for obtaining red and white quality wines were selected for this purpose. Four different experiments, with different doses of AlCl3 aqueous solution, were initiated in order to determine the optimal concentration of aluminium chloride solution, which would lead to the accumulation of large amounts of resveratrol in the vine plants. Key words: resveratrol, elicitors, (grape-) vine Rezumat. Se cunoaşte faptul că, viţa de vie poate sintetiza, ca răspuns la un stres (agenţi elicitori fizici sau chimici, o infecţie parazitară) molecule naturale, denumite generic fitoalexine, care îi permit să se adapteze acestui stres. Polifenolii stilbenici reprezintă constituenţi importanţi ai acestor molecule, resveratrolul (trans-3,5,4-trihidroxistilben), fiind reprezentantul principal. Printre agenţii chimici capabili să inducă sinteza resveratrolului în plantele de viţă-de-vie cu care vin în contact, clorura de aluminiu este cea mai eficientă. Studiul efectuat a avut scopul de a determina dozele active utilizabile de AlCl 3, capabile să stimuleze biosinteza polifenolilor şi, implicit a resveratrolului, în plantele de viţă-de-vie, în condiţii controlate in vivo. În acest scop au fost selectate câteva genotipuri Vitis vinifera L. destinate obţinerii de vinuri roşii şi albe de calitate. În vederea stabilirii concentraţiei optime de soluţie de clorură de aluminiu, care să conducă la acumularea unor cantităţi importante de resveratrol în plantele de viţă de vie, s-au iniţiat patru variante experimentale, utilizându-se doze diferite de soluţie apoasă de AlCl 3. Cuvinte cheie: resveratrol, agenţi elicitori, viţă-de-vie. 1 National Institute of Research and Development for Biotechnology in Horticulture Stefanesti - Arges
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ISSN-L=1454-7376 (Print)-ISSN 1454-
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13. CIORNEA 1 Elena, TUTU Elena, CO
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38. 39. 40. 41. 42. 43. 44. 45. 46.
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64. FILIMON V.R., NICULAUA M., MIHA
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91. NEGREA Roxana, ZLATI Cristina -
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12. BEJAN 1 Carmen, VIŞOIU Emilia
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38. 39. 40. 41. 42. 43. 44. 45. 46.
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64. 65. 66. 67. 68. 69. 70. 71. 72.
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compoziţii vegetale pentru amenaj
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INTRODUCTION Due to the increased f
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Salicylates provided an increased y
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total mass. However, it is required
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with mineral fertilizer consumption
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2. In the second experiment the spr
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CONCLUSIONS 1. The extraction of sp
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INTRODUCTION Lately, one may notice
Page 34 and 35: ions leads to a retention rate grow
Page 36 and 37: CONCLUSIONS 1. The stability of tre
Page 38 and 39: fundamental process in coupled dyna
Page 40 and 41: x� 1 � x 2 x� 2 � x� 3
Page 42 and 43: Fig. 7 - (2y1(t) -, x1(t) -, for p=
Page 44 and 45: showed that the existence of fracta
Page 46 and 47: Fig. 3 - The fractal dimension for
Page 48 and 49: From figure 6 we can see that the e
Page 50 and 51: INTRODUCTION Nichel as ultramicroel
Page 52 and 53: Table 2 POD activity (conventional
Page 54 and 55: REFERENCES 1. Brown P.H., Welch R.M
Page 56 and 57: Actually, SSR markers are considere
Page 58 and 59: Table 1 (continuation) Inherintance
Page 60 and 61: Drofa Rf genotype was positioned in
Page 62 and 63: Center for Biotechnology Informatio
Page 64 and 65: Fig. 2 - RRM_1- RNA recognition mot
Page 66 and 67: 4. Mackenzie S.A., McIntosh L., 199
Page 68 and 69: MATERIAL AND METHOD Six fertility r
Page 70 and 71: Fig. 1 - Densitometry analysis of O
Page 72 and 73: CONCLUSIONS 1. Screening of three O
Page 74 and 75: The first flow cytometers quantifie
Page 76 and 77: Our data confirm the previous resul
Page 78 and 79: 7. Dydak M., Kolano B., Nowak T., S
Page 80 and 81: prezenţa şi tipul aberaţiilor cr
Page 82 and 83: The value of the mitotic index was
Page 86 and 87: INTRODUCTION Like the other plants,
Page 88 and 89: Fig. 3 - Histogram representing the
Page 90 and 91: Among the tested control plants, th
Page 92 and 93: their control techniques. The main
Page 94 and 95: figure 2, and, in their analysis it
Page 96 and 97: CONCLUSIONS The analysis of experim
Page 98 and 99: metabolism can be easily detected b
Page 100 and 101: PAL activity (∆E/mg prot.) PAL ac
Page 102 and 103: Academiei de Ştiinţe a Moldovei.
Page 104 and 105: efectuate investigaţii biochimice
Page 106 and 107: UI compared to 31,53 UI, the value
Page 108 and 109: 3. The evolution of superoxide dism
Page 110 and 111: the pyridazine derivatives in growt
Page 112 and 113: Pyridazine derivatives tested did n
Page 114 and 115: 8. Drochioiu G., Maftei M., Mangala
Page 116 and 117: according to the classification to
Page 118 and 119: formazan/g, a mixture of micronutri
Page 120 and 121: 4. At 7 days after inoculation of t
Page 122 and 123: the root types of vascular bundles
Page 124 and 125: cells disposed more compacted at su
Page 126 and 127: CONCLUSIONS 1. The general structur
Page 128 and 129: INTRODUCTION Never in the history o
Page 130 and 131: Attracting social partners in the e
Page 132 and 133: CONCLUSIONS 1. Evaluator and tutors
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firms seek resources and new ways t
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companies in the wine sector. Indic
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From the analysis of figure 1 can b
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warning about the practice of copyi
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success to an extent far greater th
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CONCLUSIONS 1. At regional level th
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team that he leads, establishing re
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Nr. crt. NAME OF CROPS Areas cultiv
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management skills necessary to admi
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personalities. The education by mea
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The final part of the activities wa
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CONCLUSIONS The activities carried
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din punct de vedere al creşterii v
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percentage of + 58 %. The productio
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The second component reflects the l
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It should be noted that the net inc
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CONCLUSIONS 1. Studies have shown t
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variety were given mineral fertiliz
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40.7 g compared to the control. In
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In super intensive orchards of spec
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3 years of fruiting, 72.9 t/ha. The
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Replacing the old varieties, that h
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Golden Reinders varieties on the br
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varieties introduced in the country
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and H. Gilbert (1843), at the Rotha
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Nr. crt. Table 1 Distances and dens
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CONCLUSIONS 1. Technological factor
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Knowledge of the main agrobiologica
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The following graph, the diameter g
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CONCLUSIONS 1. The analysis main mo
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Vegetable production is marked by a
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vegetable sprouts. Optimum growing
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Ecological and physiological charac
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MATERIAL AND METHOD The carrying ou
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Question: “Would you buy ecologic
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Thus, 12.9% out of the persons inte
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MATERIAL AND METHOD The biological
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No crt. 1 Results regarding the fre
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CONCLUSIONS 1. The meteorological-p
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MATERIAL AND METHOD The research wa
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In the stationary microfarm Maxim o
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Tomato Granadero F1 row Tomato Gran
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MATERIAL AND METHOD The breeding wo
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No. Followed character The main cha
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L9 L10 Mt Fig. 1 - The selection of
Page 224 and 225:
INTRODUCTION Increasing demand for
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REFERENCES 1. Gozob T., Micu Chiria
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of reduced energy consumption, have
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The results obtained for apples pro
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Pruning influence on apple producti
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mahaleb succeeds in the NE of Roman
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in the maturation stadium, have cra
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its acidity covers an intermediary
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eacts with the basic solution, the
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and mildew vulnerability, obtained
Page 244 and 245:
Analyzing data on the amount of bio
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INTRODUCTION Numerous scientists ar
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- Arthemisia annua, cultivated in c
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much more decorative, framing very
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potential, were realised both on na
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flowers grouped in umbelliform infl
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3. Function of ornamental features
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and have species with different eco
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It is cultivated as ornamental gras
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„Silberfeder Miscanthus sinensis
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INTRODUCTION Located in the SE of R
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3. Sedum urvillei DC. (sin. Sedum s
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CONCLUSIONS 1. The spontaneous flor
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1. - the bush shape; 2. - the vigou
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Table 4 The quality evaluation shee
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value of roses. Through this we can
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three meters between rows and one m
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Table 3 Fruit production of apple c
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CONCLUSIONS The production of apple
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INTRODUCTION Researches whose resul
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The spatial distribution of suitabi
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3. The most favorable for the wine
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MATERIAL AND METHOD The ecological
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CONCLUSIONS The ecological index ca
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of rose wines differing according t
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The changes of color components are
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Blending means - beyond the combina
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The V[p,s] property values for the
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No major changes of the hierarchy a
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another essential condition: the ca
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having a fish tradition, for a cons
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The environment conditions may favo
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Fig. 1 - Chemical formula of vitami
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substances with a protective role d
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~ Is allergic activities (www.trata
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and Grădinariu G, 2000), cherry pr
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Fruits moisture ranged between 79.6
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5. The data obtained, are within th
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Plums contain large quantities of s
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Regarding the fact that all cultiva
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other compounds, while at the Dâmb
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statistic-mathematical multi-variat
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In order to ensure more fidelity in
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INTRODUCTION Climate change has an
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Fig. 2 - TblClima table structure T
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and provides information on phenolo
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INTRODUCTION It is a common knowled
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A tendency towards enhancement of l
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At the same time, the results of yi
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phénolique constitue une donne ind
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plus petites que pur le Merlot ou l
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Suite aux dégustations organolépt
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wines. A fourth variety, Gewürtztr
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The highest quantities are found in
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CONCLUSIONS 1. In Tămâioasă rom
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INTRODUCTION The physical-chemical
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N o Technologi cal variant Alcoho l
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REFERENCES 1. Cotea D.V., 1985 - Tr
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asic wine and the yeasts triggering
Page 362 and 363:
Fig. 2 - Dynamics of reducing sugar
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Pressure was determined since the 1
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character that may be frequently fo
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To totally eliminate the germs of b
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Aluminum vessels used for the prepa
Page 372 and 373:
Influence of technological phases o
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Table 4 Losses of vitamin C recorde
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ascorbic, E301 sodium ascorbate, E3
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anthocyanin pigments, flavonoids, t
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economically speaking, due to very
Page 382 and 383:
REFERENCES 1. Adams J. B., 1973 - T
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with ascorbic acid are major antiox
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cabbages, which have antioxidant ac
Page 388 and 389:
5. Correlation between total phenol
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MATERIAL AND METHOD Experiences wer
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compared to the control and lowest
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and development, increased producti
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Conversely, the cost increases and
Page 398 and 399:
CONCLUSIONS 1. In the central area
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INTRODUCTION The physical degradati
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The laboratory test rig has the fol
Page 404 and 405:
Acknowledgements. The laboratory te
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Factor B = NITROGEN (kg N / ha): b1
Page 408 and 409:
Table 3 Influence of nitrogen and p
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4. Regarding the influence of incre
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the two objective and important cha
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- very hard summer consistency on s
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weather and antropic factors of the
Page 418 and 419:
Wander and co., 2002 considers that
Page 420 and 421:
Ecological climatic factors Average
Page 422 and 423:
7. Januszek K, 1999 - Actywnosc enz
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using the most suitable rootstocks
Page 426 and 427:
Soil porosity. Large pore diverse l
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low, which does not influence the d
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2005, 2009 a, 2009 b, Borza I. et a
Page 432 and 433:
Table 5 The total water consumption
Page 434 and 435:
CONCLUSIONS 1. At unirrigated potat
Page 436 and 437:
irrigation systems the paper studie
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The crop coefficients (Kc) for the
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for respectively month. The differe
Page 442 and 443:
MATERIAL AND METHOD In order to per
Page 444 and 445:
In order to facilitate the access o
Page 446 and 447:
The water sources absorbed by the b
Page 448 and 449:
compaction of the cultivated horizo
Page 450 and 451:
machinery wheals, we can observe a
Page 452 and 453:
CONCLUSIONS 1. The increase of the
Page 454 and 455:
These compounds are efficient in co
Page 456 and 457:
CONCLUSIONS The fight against this
Page 458 and 459:
INTRODUCTION Timis River, the riche
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Gavojdia sampling point is located
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0.7 0.6 0.5 0.4 0.3 0.2 0.1 0 0 0.0
Page 464 and 465:
Recording as changes in the structu
Page 466 and 467:
The earthworms species collected in
Page 468 and 469:
Page 470 and 471:
In the Farm Rediu Iasi from table 3
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36 63 472 82 Vasile Adamachi Rediu
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gathered from May to July. Gatherin
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-on first harvest on 26.05 I collec
Page 478 and 479:
6 06.06 Folicur Actelic 7 13.06 Cla
Page 480 and 481:
MATERIAL AND METHOD The biological
Page 482 and 483:
The differences from the three-year
Page 484 and 485:
The analysis of data recorded in ta
Page 486 and 487:
Page 488 and 489:
Nr. Phenologic phase 1 White button
Page 490 and 491:
It has taken this choice, because i
Page 492 and 493:
et al., 2007) and convert the dange
Page 494 and 495:
According to data from table 3 the
Page 496 and 497:
MATERIAL AND METHOD The experiences
Page 498 and 499:
From the second year of culture, pl
Page 500 and 501:
During this study it has been found
Page 502 and 503:
MATERIAL AND METHOD The two farms s
Page 504 and 505:
The project contains these measures
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and of downstream dams that, beginn
Page 508 and 509:
and urban creations, we link this w
Page 510 and 511:
Also, he is one of the first creato
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3. In the development of such proje
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archeological signs with the urbani
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As long as the towns of medieval Mo
Page 518 and 519:
in the palace, centrally located, o
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the job or to other interest points
Page 522 and 523:
The modernization of these public m
Page 524 and 525:
CONCLUSIONS The concept represents
Page 526 and 527:
1975; Enache I., 2002). Research co
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Fig.3 - Analysis of the model As a
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made of plastic (for economic model
Page 532 and 533:
The simplicity of the effect is alw
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a) Pyrus calleryana b) Pyrus amygda
Page 536 and 537:
The quantity of colour plays an imp
Page 538 and 539:
unique, colourful foliage, like the
Page 540 and 541:
Another decorative specie is Japane
Page 542 and 543:
foliage greens, but in other colour
Page 544 and 545:
imagination was regarded as having
Page 546 and 547:
specific way of interpreting realit
Page 548 and 549:
MATERIAL AND METHOD The plant mater
Page 550 and 551:
following varieties in the South of
Page 552 and 553:
contents of the above compounds is
Page 554 and 555:
a number of about 40 ample windthro
Page 556 and 557:
Fig. 2 - EEF index values before wi
Page 558 and 559:
ecoprotective effectiveness is main
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independence, too (Adams S. et al.,
Page 562 and 563:
RESULTS AND DISCUSSIONS Results of
Page 564 and 565:
2,3°C the specific cost is 16,07
Page 566 and 567:
INTRODUCTION Dealu Bujorului vineya
Page 568 and 569:
In August, when the grape vine begi
Page 570 and 571:
So, the wines made by maceration-fe
Page 572 and 573:
Forest "Dumbrava Sibiului" in 1900-
Page 574 and 575:
Family P A P I L I O N I D A E Genu
Page 576 and 577:
thousands of collection of this dat
Page 578:
Editorial Consultant: Vasile VÎNTU
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LUCRĂRI ŞTIINŢIFICE Vol. 54 NR. 2 SERIA HORTICULTURĂ

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