LUCRĂRI ŞTIINŢIFICE Vol. 54 NR. 2 SERIA HORTICULTURĂ

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4. Mackenzie S.A., McIntosh L., 1999 – Higher plant mitochondria. Plant Cell; vol. 11, p. 571-586. 5. Midoni A. 2010 – Identificarea şi expresia unor gene (Rf) la floarea-soarelui (Helianthus annuus L.), Autoreferatul tezei de doctor. Chişinău, p. 29; 6. Turbin N.V., Palilova A.N., 1970 – Teoreticescaia modeli vzaimodeistvia hromosomnih ghenov i citoplasmi pri CMS u cucuruzi. Dokladi VASHNIL; nr. 2, p. 2-4. 7. http://blast.ncbi.nlm.nih.gov/Blast.cgi; 8. PhyloDraw Ver. 0.8 Graphics Application Lab., Pusan National University; 9. www.attotron.com/cybertory/analysis/trans.htm; 10. www.clcbio.com; 11. www.ebi.ac.uk/Tools/clustalw2; 12. www.ncbi.nlm.nih.gov; 13. www.pbil.univ-lyon1.fr/cap3.php; 14. www.pfam.sanger.ac.uk/family.acc. 66
GENETIC DISTANCE EVALUATION, A USEFUL TOOL IN HETEROSIS EFFECT PREDICTION OF SUNFLOWER GENOTIPES EVALUAREA DISTANŢEI GENETICE ÎN SCOPUL PRONOSTICĂRII EFECTULUI DE HETEROZIS LA DIVERSE GENOTIPURI DE FLOAREA-SOARELUI PORT Angela 1 , LEVIŢCHI A. 1 , DUCA Maria 1 , MIDONI A. 1 , CHILARI Rodica 1 e-mail: mduca2000@yahoo.com Abstract. The goal of the research was to assess the RAPD banding pattern among different cytoplasmic male sterile, fertility restorer lines and hybrid sunflower genotypes to be associated with restorer of fertility and heterotic traits. It is discussed the efficiency of parental selection based on genetic distance by RAPD polymorphisms clustering. Also, screening of three Operon primers, previously reported as polymorphic, revealed four amplification products (OPG10 510; OPG10 680; OPI16 450 and OPI16 550) specific only for studied Rf lines, suggesting on their potential use for indirect selection of fertility restorer trait. Key words: sunflower, CMS lines, Rf lines, heterosis effect, hybrids Rezumat. Scopul cercetării a constat în evaluarea polimorfismului genetic la diverse genotipuri ASC, Rf şi F 1 pentru evidenţierea gradului de heterozis şi identificării unor potenţiali markeri asociaţi cu capacitatea de restaurare a fertilitîţii polenului. Este discutată eficienţa de selecţie a liniilor parentale utilizând distanţa genetică în baza profilelor RAPD. Totodată, au fost identificaţi ampliconi RAPD (OPG 10 510, OPG 10 680, OPI 16 450 şi OPI 16 550) specifici liniilor restauratoare care ulterior, pot fi utilizaţi în elaborarea markerilor moleculari linckaţi cu acest caracter. Cuvinte cheie: floarea-soarelui, linii ASC, linii Rf, vigoare hibridă, hibrizi INTRODUCTION The RAPD (Random Amplification of Polymorphic DNA) technique is successfully used from various laboratories to investigate the genetic polymorphism, especially due to the experiment low cost. Although, other marcher technology are considered more reliable and reproducible, the good experimental optimization could assure the effective parent selection for hybrid breeding related various important qualitative and quantitative traits (productivity, high resistance to different stress factors). The objective of this study was to assess the genetic distance based on RAPD polymorphism with OPG10, OPI16 and OPH15 primers among different cytoplasmic male sterile, fertility restorer lines and hybrid sunflower genotypes. 1 University of Academy of Sciences of Moldova, Republic of Moldova 67
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ISSN-L=1454-7376 (Print)-ISSN 1454-
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13. CIORNEA 1 Elena, TUTU Elena, CO
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38. 39. 40. 41. 42. 43. 44. 45. 46.
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64. FILIMON V.R., NICULAUA M., MIHA
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91. NEGREA Roxana, ZLATI Cristina -
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12. BEJAN 1 Carmen, VIŞOIU Emilia
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38. 39. 40. 41. 42. 43. 44. 45. 46.
Page 16 and 17: 64. 65. 66. 67. 68. 69. 70. 71. 72.
Page 18 and 19: compoziţii vegetale pentru amenaj
Page 20 and 21: INTRODUCTION Due to the increased f
Page 22 and 23: Salicylates provided an increased y
Page 24 and 25: total mass. However, it is required
Page 26 and 27: with mineral fertilizer consumption
Page 28 and 29: 2. In the second experiment the spr
Page 30 and 31: CONCLUSIONS 1. The extraction of sp
Page 32 and 33: INTRODUCTION Lately, one may notice
Page 34 and 35: ions leads to a retention rate grow
Page 36 and 37: CONCLUSIONS 1. The stability of tre
Page 38 and 39: fundamental process in coupled dyna
Page 40 and 41: x� 1 � x 2 x� 2 � x� 3
Page 42 and 43: Fig. 7 - (2y1(t) -, x1(t) -, for p=
Page 44 and 45: showed that the existence of fracta
Page 46 and 47: Fig. 3 - The fractal dimension for
Page 48 and 49: From figure 6 we can see that the e
Page 50 and 51: INTRODUCTION Nichel as ultramicroel
Page 52 and 53: Table 2 POD activity (conventional
Page 54 and 55: REFERENCES 1. Brown P.H., Welch R.M
Page 56 and 57: Actually, SSR markers are considere
Page 58 and 59: Table 1 (continuation) Inherintance
Page 60 and 61: Drofa Rf genotype was positioned in
Page 62 and 63: Center for Biotechnology Informatio
Page 64 and 65: Fig. 2 - RRM_1- RNA recognition mot
Page 68 and 69: MATERIAL AND METHOD Six fertility r
Page 70 and 71: Fig. 1 - Densitometry analysis of O
Page 72 and 73: CONCLUSIONS 1. Screening of three O
Page 74 and 75: The first flow cytometers quantifie
Page 76 and 77: Our data confirm the previous resul
Page 78 and 79: 7. Dydak M., Kolano B., Nowak T., S
Page 80 and 81: prezenţa şi tipul aberaţiilor cr
Page 82 and 83: The value of the mitotic index was
Page 84 and 85: CONCLUSIONS 1. The value of the mit
Page 86 and 87: INTRODUCTION Like the other plants,
Page 88 and 89: Fig. 3 - Histogram representing the
Page 90 and 91: Among the tested control plants, th
Page 92 and 93: their control techniques. The main
Page 94 and 95: figure 2, and, in their analysis it
Page 96 and 97: CONCLUSIONS The analysis of experim
Page 98 and 99: metabolism can be easily detected b
Page 100 and 101: PAL activity (∆E/mg prot.) PAL ac
Page 102 and 103: Academiei de Ştiinţe a Moldovei.
Page 104 and 105: efectuate investigaţii biochimice
Page 106 and 107: UI compared to 31,53 UI, the value
Page 108 and 109: 3. The evolution of superoxide dism
Page 110 and 111: the pyridazine derivatives in growt
Page 112 and 113: Pyridazine derivatives tested did n
Page 114 and 115: 8. Drochioiu G., Maftei M., Mangala
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according to the classification to
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formazan/g, a mixture of micronutri
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4. At 7 days after inoculation of t
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the root types of vascular bundles
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cells disposed more compacted at su
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CONCLUSIONS 1. The general structur
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INTRODUCTION Never in the history o
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Attracting social partners in the e
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CONCLUSIONS 1. Evaluator and tutors
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firms seek resources and new ways t
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companies in the wine sector. Indic
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From the analysis of figure 1 can b
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warning about the practice of copyi
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success to an extent far greater th
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CONCLUSIONS 1. At regional level th
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team that he leads, establishing re
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Nr. crt. NAME OF CROPS Areas cultiv
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management skills necessary to admi
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personalities. The education by mea
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The final part of the activities wa
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CONCLUSIONS The activities carried
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din punct de vedere al creşterii v
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percentage of + 58 %. The productio
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The second component reflects the l
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It should be noted that the net inc
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CONCLUSIONS 1. Studies have shown t
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variety were given mineral fertiliz
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40.7 g compared to the control. In
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In super intensive orchards of spec
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3 years of fruiting, 72.9 t/ha. The
Page 176 and 177:
Replacing the old varieties, that h
Page 178 and 179:
Golden Reinders varieties on the br
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varieties introduced in the country
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and H. Gilbert (1843), at the Rotha
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Nr. crt. Table 1 Distances and dens
Page 186 and 187:
CONCLUSIONS 1. Technological factor
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Knowledge of the main agrobiologica
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The following graph, the diameter g
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CONCLUSIONS 1. The analysis main mo
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Vegetable production is marked by a
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vegetable sprouts. Optimum growing
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Ecological and physiological charac
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MATERIAL AND METHOD The carrying ou
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Question: “Would you buy ecologic
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Thus, 12.9% out of the persons inte
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MATERIAL AND METHOD The biological
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No crt. 1 Results regarding the fre
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CONCLUSIONS 1. The meteorological-p
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MATERIAL AND METHOD The research wa
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In the stationary microfarm Maxim o
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Tomato Granadero F1 row Tomato Gran
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MATERIAL AND METHOD The breeding wo
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No. Followed character The main cha
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L9 L10 Mt Fig. 1 - The selection of
Page 224 and 225:
INTRODUCTION Increasing demand for
Page 226 and 227:
REFERENCES 1. Gozob T., Micu Chiria
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of reduced energy consumption, have
Page 230 and 231:
The results obtained for apples pro
Page 232 and 233:
Pruning influence on apple producti
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mahaleb succeeds in the NE of Roman
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in the maturation stadium, have cra
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its acidity covers an intermediary
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eacts with the basic solution, the
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and mildew vulnerability, obtained
Page 244 and 245:
Analyzing data on the amount of bio
Page 246 and 247:
INTRODUCTION Numerous scientists ar
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- Arthemisia annua, cultivated in c
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much more decorative, framing very
Page 252 and 253:
potential, were realised both on na
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flowers grouped in umbelliform infl
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3. Function of ornamental features
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and have species with different eco
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It is cultivated as ornamental gras
Page 262 and 263:
„Silberfeder Miscanthus sinensis
Page 264 and 265:
INTRODUCTION Located in the SE of R
Page 266 and 267:
3. Sedum urvillei DC. (sin. Sedum s
Page 268 and 269:
CONCLUSIONS 1. The spontaneous flor
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1. - the bush shape; 2. - the vigou
Page 272 and 273:
Table 4 The quality evaluation shee
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value of roses. Through this we can
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three meters between rows and one m
Page 278 and 279:
Table 3 Fruit production of apple c
Page 280 and 281:
CONCLUSIONS The production of apple
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INTRODUCTION Researches whose resul
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The spatial distribution of suitabi
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3. The most favorable for the wine
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MATERIAL AND METHOD The ecological
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CONCLUSIONS The ecological index ca
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of rose wines differing according t
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The changes of color components are
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Blending means - beyond the combina
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The V[p,s] property values for the
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No major changes of the hierarchy a
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another essential condition: the ca
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having a fish tradition, for a cons
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The environment conditions may favo
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Fig. 1 - Chemical formula of vitami
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substances with a protective role d
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~ Is allergic activities (www.trata
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and Grădinariu G, 2000), cherry pr
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Fruits moisture ranged between 79.6
Page 318 and 319:
5. The data obtained, are within th
Page 320 and 321:
Plums contain large quantities of s
Page 322 and 323:
Regarding the fact that all cultiva
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other compounds, while at the Dâmb
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statistic-mathematical multi-variat
Page 328 and 329:
In order to ensure more fidelity in
Page 330 and 331:
INTRODUCTION Climate change has an
Page 332 and 333:
Fig. 2 - TblClima table structure T
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and provides information on phenolo
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INTRODUCTION It is a common knowled
Page 338 and 339:
A tendency towards enhancement of l
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At the same time, the results of yi
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phénolique constitue une donne ind
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plus petites que pur le Merlot ou l
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Suite aux dégustations organolépt
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wines. A fourth variety, Gewürtztr
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The highest quantities are found in
Page 352 and 353:
CONCLUSIONS 1. In Tămâioasă rom
Page 354 and 355:
INTRODUCTION The physical-chemical
Page 356 and 357:
N o Technologi cal variant Alcoho l
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REFERENCES 1. Cotea D.V., 1985 - Tr
Page 360 and 361:
asic wine and the yeasts triggering
Page 362 and 363:
Fig. 2 - Dynamics of reducing sugar
Page 364 and 365:
Pressure was determined since the 1
Page 366 and 367:
character that may be frequently fo
Page 368 and 369:
To totally eliminate the germs of b
Page 370 and 371:
Aluminum vessels used for the prepa
Page 372 and 373:
Influence of technological phases o
Page 374 and 375:
Table 4 Losses of vitamin C recorde
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ascorbic, E301 sodium ascorbate, E3
Page 378 and 379:
anthocyanin pigments, flavonoids, t
Page 380 and 381:
economically speaking, due to very
Page 382 and 383:
REFERENCES 1. Adams J. B., 1973 - T
Page 384 and 385:
with ascorbic acid are major antiox
Page 386 and 387:
cabbages, which have antioxidant ac
Page 388 and 389:
5. Correlation between total phenol
Page 390 and 391:
MATERIAL AND METHOD Experiences wer
Page 392 and 393:
compared to the control and lowest
Page 394 and 395:
and development, increased producti
Page 396 and 397:
Conversely, the cost increases and
Page 398 and 399:
CONCLUSIONS 1. In the central area
Page 400 and 401:
INTRODUCTION The physical degradati
Page 402 and 403:
The laboratory test rig has the fol
Page 404 and 405:
Acknowledgements. The laboratory te
Page 406 and 407:
Factor B = NITROGEN (kg N / ha): b1
Page 408 and 409:
Table 3 Influence of nitrogen and p
Page 410 and 411:
4. Regarding the influence of incre
Page 412 and 413:
the two objective and important cha
Page 414 and 415:
- very hard summer consistency on s
Page 416 and 417:
weather and antropic factors of the
Page 418 and 419:
Wander and co., 2002 considers that
Page 420 and 421:
Ecological climatic factors Average
Page 422 and 423:
7. Januszek K, 1999 - Actywnosc enz
Page 424 and 425:
using the most suitable rootstocks
Page 426 and 427:
Soil porosity. Large pore diverse l
Page 428 and 429:
low, which does not influence the d
Page 430 and 431:
2005, 2009 a, 2009 b, Borza I. et a
Page 432 and 433:
Table 5 The total water consumption
Page 434 and 435:
CONCLUSIONS 1. At unirrigated potat
Page 436 and 437:
irrigation systems the paper studie
Page 438 and 439:
The crop coefficients (Kc) for the
Page 440 and 441:
for respectively month. The differe
Page 442 and 443:
MATERIAL AND METHOD In order to per
Page 444 and 445:
In order to facilitate the access o
Page 446 and 447:
The water sources absorbed by the b
Page 448 and 449:
compaction of the cultivated horizo
Page 450 and 451:
machinery wheals, we can observe a
Page 452 and 453:
CONCLUSIONS 1. The increase of the
Page 454 and 455:
These compounds are efficient in co
Page 456 and 457:
CONCLUSIONS The fight against this
Page 458 and 459:
INTRODUCTION Timis River, the riche
Page 460 and 461:
Gavojdia sampling point is located
Page 462 and 463:
0.7 0.6 0.5 0.4 0.3 0.2 0.1 0 0 0.0
Page 464 and 465:
Recording as changes in the structu
Page 466 and 467:
The earthworms species collected in
Page 468 and 469:
Page 470 and 471:
In the Farm Rediu Iasi from table 3
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36 63 472 82 Vasile Adamachi Rediu
Page 474 and 475:
gathered from May to July. Gatherin
Page 476 and 477:
-on first harvest on 26.05 I collec
Page 478 and 479:
6 06.06 Folicur Actelic 7 13.06 Cla
Page 480 and 481:
MATERIAL AND METHOD The biological
Page 482 and 483:
The differences from the three-year
Page 484 and 485:
The analysis of data recorded in ta
Page 486 and 487:
Page 488 and 489:
Nr. Phenologic phase 1 White button
Page 490 and 491:
It has taken this choice, because i
Page 492 and 493:
et al., 2007) and convert the dange
Page 494 and 495:
According to data from table 3 the
Page 496 and 497:
MATERIAL AND METHOD The experiences
Page 498 and 499:
From the second year of culture, pl
Page 500 and 501:
During this study it has been found
Page 502 and 503:
MATERIAL AND METHOD The two farms s
Page 504 and 505:
The project contains these measures
Page 506 and 507:
and of downstream dams that, beginn
Page 508 and 509:
and urban creations, we link this w
Page 510 and 511:
Also, he is one of the first creato
Page 512 and 513:
3. In the development of such proje
Page 514 and 515:
archeological signs with the urbani
Page 516 and 517:
As long as the towns of medieval Mo
Page 518 and 519:
in the palace, centrally located, o
Page 520 and 521:
the job or to other interest points
Page 522 and 523:
The modernization of these public m
Page 524 and 525:
CONCLUSIONS The concept represents
Page 526 and 527:
1975; Enache I., 2002). Research co
Page 528 and 529:
Fig.3 - Analysis of the model As a
Page 530 and 531:
made of plastic (for economic model
Page 532 and 533:
The simplicity of the effect is alw
Page 534 and 535:
a) Pyrus calleryana b) Pyrus amygda
Page 536 and 537:
The quantity of colour plays an imp
Page 538 and 539:
unique, colourful foliage, like the
Page 540 and 541:
Another decorative specie is Japane
Page 542 and 543:
foliage greens, but in other colour
Page 544 and 545:
imagination was regarded as having
Page 546 and 547:
specific way of interpreting realit
Page 548 and 549:
MATERIAL AND METHOD The plant mater
Page 550 and 551:
following varieties in the South of
Page 552 and 553:
contents of the above compounds is
Page 554 and 555:
a number of about 40 ample windthro
Page 556 and 557:
Fig. 2 - EEF index values before wi
Page 558 and 559:
ecoprotective effectiveness is main
Page 560 and 561:
independence, too (Adams S. et al.,
Page 562 and 563:
RESULTS AND DISCUSSIONS Results of
Page 564 and 565:
2,3°C the specific cost is 16,07
Page 566 and 567:
INTRODUCTION Dealu Bujorului vineya
Page 568 and 569:
In August, when the grape vine begi
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So, the wines made by maceration-fe
Page 572 and 573:
Forest "Dumbrava Sibiului" in 1900-
Page 574 and 575:
Family P A P I L I O N I D A E Genu
Page 576 and 577:
thousands of collection of this dat
Page 578:
Editorial Consultant: Vasile VÎNTU
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LUCRĂRI ŞTIINŢIFICE Vol. 54 NR. 2 SERIA HORTICULTURĂ

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