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Conclusion: This is the first study in a reliable large animal transplant model demonstrating the efficacy
of a simple cold oxygenated machine perfusion system to rescue, otherwise lethal, ischemic injured
DCD liver grafts.
37.4
Detecting rejection after mouse islet transplantation utilizing islet protein-stimulated ELISPOT
C. Toso 1 , R. Pawlick 2 , R. Edgar 2 , J. Davis 2 , M. McCall 2 , T. Berney 1 , J. Shapiro 2 ( 1 Geneva, 2 Edmonton/
CDN)
Objective: The development of blood-based monitoring tests allowing for the detection of rejection
would improve post-transplant management, with the potential for better outcome. The present study
explored the possibility of detecting harmful events after mouse islet transplantation measuring the
immune responsiveness against islet proteins.
Methods: Mouse islet transplantations were performed using various donor/recipient combinations,
exploring autoimmune (NOD/SCID to NOD, n=6), alloimmune events (C57/Bl6 to BALB/c, n=20), a
combination of both (C57/Bl6 to NOD, n=8), the absence of both (BALB/c to BALB/c, n=21) or naïve,
non-transplanted control mice (n=14). The immune reactivity was measured by ELISPOT, looking at the
ex vivo release of _ ;IFN from splenocytes stimulated by islet donor proteins (sonicated islets).
Results: The various explored transplant models demonstrated significantly differing graft survivals
(p≤0.001, log-rank). The ex vivo immune reactivity was not altered in the syngeneic and autoimmune
models, demonstrating similar levels as non-transplanted controls (p=1 in both cases, Bonferroni’s
post-hoc test on ranks). Conversely, the occurrence of an allogeneic rejection alone or in combination
to autoimmunity was associated to an increase in the level of immune reactivity (p=0.012 and p=0.004
vs. respective controls). The observed increase was transient and lost in the post-rejection period in the
C57/Bl6 to NOD model (p=1 vs. non-transplanted controls).
Conclusion: In this study allogeneic rejection (and not autoimmune recurrence) was associated to a
transient increase in the reactivity of splenocytes against islet proteins. Such a strategy has the potential
for improving post-transplant graft monitoring in human and should be further explored.
37.5
Liraglutide, a long-acting human glucagon-like peptide 1 analogue, improves human islet survival
in culture
C. Toso 1 , M. McCall 2 , J. Emamaullee 2 , S. Merani 2 , J. Davis 2 , R. Edgar 2 , R. Pawlick 2 , T. Kin 2 , L. B. Knudsen 3 ,
J. Shapiro 2 ( 1 Geneva, 2 Edmonton/CDN, 3 Bagsvaerd/DK)
Objective: The culture of human islets is associated with approximately 10-20% islet loss, occasionally
preventing transplantation. Preconditioning of the islets to improve postculture yields would be of
immediate benefit, with the potential to increase both the number of transplanted patients and their
metabolic reserve.
Methods: In this study, the effect of liraglutide, a long-acting human glucagon-like peptide 1 analogue,
on cultured human islets was examined.
Results: Culture with liraglutide (1 mumol/l) was associated with a preservation of islet mass (significantly
more islets at 24 and 48 h, compared to control; P