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Acute limb ischemia timing for experimental assessment of translational reperfusion protocols in an
in-vivo rodent model
R. von Allmen, F. Dick, Y. Banz, J. Li, J. Schmidli, H. Tevaearai (Berne)
Objective: Controlled reperfusion is a promising posthoc strategy to reduce early reperfusion injury
after acute limb ischemia. Before experimental protocols can be transferred to the clinic, however, optimal
timing of in-vivo ischemia models needs to be established for assessment of sustained efficacy.
Methods: Increasing periods of arterial hind limb ischemia (2 to 5h, 1h increments) were induced
in Wistar rats. Reperfusion was either controlled experimentally (cooled heparin solution at low perfusion-pressure
for 20 min; experimental group) or normal circulation was reinstated directly (shock
reperfusion; controls). Severity of skeletal muscle injury was assessed immediately (no reperfusion,
ischemic injury only), after 4h (early tissue injury) and after 24h. Results were adjusted to contralateral,
non-ischemic limbs and are given as median (interquartile range).
Results: A total of 49 rats was operated. Short ischemia (