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5200 Afr. J. Microbiol. Res. Table 2. Characteristics of isolates for K. pneumoniaee, E. coli, C. freundii and their E. coli transconjugants. Donor bacteria Amino acid point mutations a K. pneumoniaee (36) 3 (3/36=8.3%) 1 (1/36=2.8%) E. coli (34) 2 (2/34=5.9%) 1 (1/34=2.9%) C. freundii (20) 1 (1/20=5.0%) 1 (1/20=5.0%) 1 (1/20=5.0%) Ala(N)2→Thr(T), Iie(I)20→Val(V), Ser(S)79→Val(V), Iie(I)142→Met(M), Iie(I)144→Thr(T), Asn(N)198→Ser(S) Arn(N)27→Leu(L), Ser(S)79→Ala(A), Arg(R)87→Ser(S), Gly(G)188→Arg(R), Val(V)212→Iie(I) Ser(S)79→Ala(A), Iie(I)142→Met(M), Val(V)212→Iie(I) Ser(S)79→Ala(A), Iie(I)142→Met(M), Ala(A)144→Thr(T), Val(V)212→Iie(I) Asp(D)11→Ala(N), Ser(S)79→Ala(A), Iie(I)142→Met(M),Gly(G)188→Arg(R),V al(V)212→Iie(I) Glu(E)20→Asp(D), Ser(S)79→Ala(A), Iie(I)142→Met(M) Asp(D)11→Ala(N), Ser(S)79→Ala(A), Iie(I)142→Met(M),Gly(G)188→Arg(R), Val(V)212→Iie(I) qnr gene MICs(μg/ml) b NAL OFL LVN CIP qnrB5 >256 8-16 4-8 2 qnrB31 >256 16 8 4 qnrB9 >256 16-32 8-16 2-4 qnrB16 >256 8-16 4-8 2-4 qnrB2 >256 8-16 4-8 2-4 qnrB15 >256 8-16 4-8 2-4 qnrB18 >256 8-32 4-8 2-4 E.coliJ53AZ R 2 0.0039 0.0019 0.0019 Transconjugants KP1-E.coliJ53 qnrB5 8-16 0.25- 0.50 0.064-0.128 0.064-0.128 KP2-E.coliJ53 qnrB31 8 0.25 0.064 0.064 EC1-E.coliJ53 qnrB9 8-16 0.25-0.50 0.064-0.128 0.064-0.128 EC2-E.coliJ53 qnrB16 16 0.50 0.128 0.128 FC1-E.coliJ53 qnrB2 16 0.25 0.064 0.064 FC2-E.coliJ53 qnrB15 16 0.50 0.128 0.128 FC3-E.coliJ53 qnrB18 8 0.25 0.064 0.064 a Amino acid point mutations were compared with qnrB1 gene (http://www.lahey.org/qnrstudies/). b NAL, nalidixic acid, OFX, ofloxacin, LVX, levofloxacin, CIP, ciprofloxacin. Program Files/Youdao/Dict4/resultui/query-result.html and C. freundii showed resistance to ofloxacin, levofloxacin, ciprofloxacin, and nalidixic acid. Furthermore, the MICs of plasmid transconjugantswere significantly higher than E. coli J53 Az R , the tests of transconjugants were successful, it illustrated that the main causes of resistance to quinolone were mediated by plasmid. The comparison and analysis of variable sites in qnrB alleles QnrB2, qnrB5, qnrB9, qnrB15, qnrB16, qnrB18 and QnrB31 that we had achieved were compared with other qnrB alleles, and the amino acid sequence diagram was made as Table 3. From Table 3, we could clearly identify the qnrB variable sites and variable
Table 3. Amino acid substitutions in qnrB1 to qnrB31 a . Allele Amino acid change at position 2 7 11 18 20 21 22 27 35 36 55 60 69 74 79 80 87 94 11 8 12 9 14 2 14 4 14 7 15 1 16 2 16 3 16 8 17 1 18 6 18 8 19 8 Wang et al. 5201 qnrB1 A G D E I E N N L S N M C A S S R A N V I A L F S T A F I G N S L M V I qnrB2 N A M R I qnrB3 K M qnrB4 T V N I N S M T S V S L M qnrB5 T V V M T S I qnrB6 A M qnrB7 A M T I qnrB8 T V I V A M T L S T A qnrB9 A M I qnrB10 T V V M T qnrB11 T A V I V S M T S V S I L M qnrB12 T A V I V S M T S V S I L qnrB13 A M R I qnrB14 D A M T I qnrB15 S A N M I qnrB16 A M T I qnrB17 M qnrB18 D A M qnrB19 T V V M T S qnrB20 N A M R qnrB21 T V I V A M T L S T S A qnrB22 T V C N I N S M T S V V S L M qnrB23 Y A M I qnrB24 M V A M qnrB25 V I V S A M T L S T S A I qnrB27 T S V A S M T A A S A qnrB28 T S V V S M T A A S A qnrB29 V A M qnrB30 S A M qnrB31 L A S M R I a Variations from the qnrB1 sequence numbered from the second potential ATG initiation codon are shown (http://www.lahey.org/qnrstudies/). 20 2 20 4 20 5 21 2 21 3
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African Journal of Microbiology Res
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Editors Prof. Dr. Stefan Schmidt Ap
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Electronic submission of manuscript
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Fees and Charges: Authors are requi
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nces Table of Contents: Volume 6 Nu
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Table of Contents: Volume 6 Number
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Table 1. Overview of the Soil prope
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exactly. MICROBIAL BIOMASS IN ORGAN
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Table 3. Advantages and disadvantag
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analysis of polymerase chain reacti
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Enzyme assay Triplicate samples of
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Figure 2. Effect of a) incubation t
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Table 2. Plasmid profile. Emerenini
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Table 1. Properties and identity of
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more understandable by the fact tha
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fertilizers considered the most imp
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Table 3. Effect of NPK levels and s
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Table 4. Effect of NPK levels and s
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Table 5. Effect of NPK levels and f
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with mineral NPK on wheat plant. Eg
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to synthetic antibiotics. In spite
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Among these, the K. pneumonia and V
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Figure 5. Pseudomonas aeruginosa An
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pathogens of their environment (She
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cultivable indigenous fishes of Ind
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Table 1. Oxidative stress parameter
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of tularemia. Ann N Y Acad. Sci., 1
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al., 1998; Heubuelt 1929) have alre
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Table 2. Influence of organic compo
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NO2 NO2 Zare et al. 5131 Figure 4.
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Sundermeyer-Klinger H, Meyer W, War
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Table 1. Composition of Mueller-Hin
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dose level, and is effective antibi
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wearing a mask is useful during the
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Novel Swine-Origin Influenza A H1N1
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that it produces lactic acid, bacte
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Figure 2. DMRT Graph, effect of var
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Page 85 and 86: Bacillus colony Fig 1. Colony morph
Page 87 and 88: thuringiensis isolate S1 (Figure 6)
Page 89 and 90: African Journal of Microbiology Res
Page 91 and 92: Figure 1. The Kinetic of P. aerugin
Page 93 and 94: Figure 3. DNA-dosimeter determined
Page 95 and 96: Relative Fluorescence Unit Figure 4
Page 97 and 98: Conclusion The public health risk i
Page 99 and 100: general are members of the normal i
Page 101 and 102: Table 2. Distribution of Nosocomial
Page 103 and 104: and also to assess the influence of
Page 105 and 106: Table 1. List of decamers used in R
Page 107 and 108: Figure 2. RAPD profile of Fusarium
Page 111 and 112: Table 1. Biosurfactant production p
Page 113 and 114: Figure 3. The correlation of reduct
Page 117 and 118: To estimate the water content, stra
Page 119 and 120: Kraiem et al. 5183 Table 2. Effect
Page 121 and 122: log CFU/g log CFU/g log CFU/g 8.5 7
Page 123 and 124: delays to cooling and wrapping on s
Page 125 and 126: pilus (tcp) that is a subtle of pol
Page 127 and 128: protein. DISCUSSION 70 kda 60 kda 5
Page 131 and 132: Percentage repellency (%) Percentag
Page 133 and 134: Fumigant toxicity of essential oils
Page 135: Table 1. Primers used for PCR and s
Page 139 and 140: composition in qnrB alleles. Althou
Page 143 and 144: Figure 1. Overview the Yongxing isl
Page 145 and 146: standard, XSLJ1, XSLJ2, XSLJ5, XSLJ
Page 147 and 148: Table 1. primer information: sequen
Page 149 and 150: conventional method. The reasons fo
Page 153 and 154: Figure 1. Comparison of amplificati
Page 155 and 156: Table 3. Fruiting-body formation an
Page 157 and 158: Yokoyama E, Yamagishi K, Hara A (20
Page 159 and 160: Table 1. Primer oligonucleotide seq
Page 161 and 162: Figure 2. Nucleotide and putative a
Page 163 and 164: Figure 6. Ghrelin expression induce
Page 167 and 168: Table 1. The SNPs related to the pr
Page 169 and 170: Figure 2. The phylogenetic trees of
Page 171 and 172: a c Rex DNA Tang et al. 5235 Figure
Page 175 and 176: Table 1. Gender and division wise d
Page 177 and 178: 62% were genotype D, A (14%), C (6%
Page 181 and 182: Swimming time (s) 1000 800 600 400
Page 183 and 184: Hepatic glycogen (mg/g) Hemoglobin
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Table 2. Sporulation index. Sign In
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Table 4. Conidial size of different
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Table 6. Sporulation of Alternaria
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Table 7. Reaction of different geno
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Table 2. Susceptibility of the clin
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Table 4. Aminoglycoside resistance
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Young, and the Councils on Clinical
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oleaginous microorganisms have been
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Table 2. Actual and predicted value
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Table 4. Analysis of variance (ANOV
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Huang et al. 5273 Figure 3. Respons
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the Central Universities (Grant No.
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Figure 1. Mechanism of enzymatic co
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Enzymatic activity (U/mL) Enzymatic
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prunin so far. By using the HPLC me
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Figure 7. Enzymatic conversion of n
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vaccine, HBs-Ab is negative in all
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Conferences and Advert August 2012
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