5136 Afr. J. Microbiol. Res. Figure 2. Structure of Zinc(II)–Nicotine complex. Table 2. Antibacterial activities of standard antibiotics, zinc(II) chloride, nicotine and the zinc(II)-nicotine complex at concentration of first and second dose levels Compound used Gentamicin Tetracycline Tobramycin Zinc (II) chloride Nicotine Zinc(II)– Nicotine complex Conc. (µg/100 µg) Gram Negative Organism Gram Positive Organism A. sabriae S. typhii S. boydii E. coli V. chlolerae P. pseudomallis P. aeroginosa B. subtilis S. aureus S. faecalis Zone of Inhibition (mm) 100 20 25 25 40 35 35 36 _ 31 _ 200 40 42 39 85 80 85 71 _ 40 _ 100 _ _ 15 10 51 _ _ 45 _ _ 200 _ _ 35 27 90 _ _ 67 _ _ 100 25 27 31 35 31 34 31 30 30 32 200 51 60 61 55 50 85 90 65 69 71 100 8 – – 8 6 6 8 – 8 6 200 10 6 6 10 8 8 10 6 9 8 100 – – – – – – – – – – 200 – – – 14 – – 14 – – 14 100 14 – 14 17 – 16 – – 15 – 200 17 – 18 18 17 18 14 17 16 –
dose level, and is effective antibiotic at the second dose level against E. coli and P. aeroginosa (Gram negative organism) and S. faecalis (Gram positive organism) with an inhibition zone of 14 mm. Photos of all inhibition zone are not shown here. Zinc-nicotine complex inhibited only five bacterial species at first concentration, however at second dose level; it inhibited the growth of eight test bacterial species. In the case of zinc (II) chloride, the zone of inhibition ranged 6 to 18 mm at first dose level and 7 to 20 mm at second dose level. Compared to nicotine alone; the zinc (II) complex of nicotine is able to inhibit almost all the studied gram positive and gram negative organisms at the higher dose level. These results are also well comparable with the three reference antibiotics that is, Gentamicin, Tetracycline and Tobramycin. Therefore, we comment that this complex is broad spectrum anti-microbial agent active against the variety of gram positive and gram negative bacterial species. Further research is underway on the antibacterial mechanism of this zinc-nicotine complex that either this is cell wall inhibitors or bactericidal or bacteriostatic. However, some researchers (Munir et al., 1994, 1995; Chohan et al., 2002) studied considerable changes in the bacterial cell membranes upon metal ion treatment, which might be one of the cause or consequence of cell death. Conclusion Zinc is relatively abundant element in biological organisms, plays an essential role in the large number of enzymatic reactions. Having the broad spectrum antimicrobial activities, zinc and its nicotine compounds may be used as a therapeutic agent and anti-sickness agent playing a role in the prevention of pain crisis in sickle-cell disease and in the treatment of various sicknesses. ACKNOWLEDGEMENTS Authors are thankful to the higher education commission, HEC, formerly named as university grant commission, UGC, for providing some financial support to this project. H.E.J. <strong>Research</strong> Institute of Chemistry, Karachi, Pakistan, is acknowledged for providing necessary instrumental facilities. REFERENCES Zaidi. 5137 Akhtar N, Malik A, Ali SN, Kazmi SU (1987). Proceragenin, an antibacterial cardenolide from Calotropic Procera. Phyto. Chem., 31: 2821-2824. Al-Tamrah SA (1999). Spectrophotometric determination of nicotine. Analytica Chimica Acta, 379: 75–80. Bayari S, Atac A, Yurdakul S (2003). Coordination behaviour of nicotinamide: an infrared spectroscopic study. J. Mol. Struct., 655: 163–170. Chohan ZH, Rauf A, Noreen S, Scozzafava A, Supuran CT (2002). Antibacterial cobalt(II), nickel(II) and zinc(II) complexes of nicotinic acid-derived Schiff-bases. J. Enzyme Inhib. Med. Chem., 17: 101– 106. Dziewulska-Kuaczkowska A, Mazur L, Ferenc W (2009).Thermal, spectroscopic and structural studies of zinc(II) complex with nicotinamide. J. Therm. Anal. Calorim., 96: 255–260. Gotti C, Zoli M, Clementi F (2006). Brain nicotinic acetylcholine receptors: native subtypes and their relevance. Trends Pharmacol. Sci., 27: 482–491. Ide S, Atac A, Yurdakul S (2002). Spectroscopic and structural studies on dichlorobis(nicotinamide)zinc(II). J. Mol. Struct., 605: 103–107. Johnson I (1994).The vinca alkaloids: A new class of oncolytic agents. Cancer Res., 12: 43–45. Levin ED, McClernon FJ, Rezvani AH (2006). Nicotinic effects on cognitive function: behavioral characterization, pharmacological specification, and anatomic localization. Psychopharmacology (Berl.), 184: 523–539. Mocchegiani E, Bertoni-Freddari C, Marcellini F, Malavolta M (2005). Brain, aging and neurodegeneration: role of zinc ion availability. Prog. Neurobiol., 75: 367–390. Munir C, Zaidi MI, Ahmad N, Rehman AR (1995). An easy rapid metal mediated method of isolation of harmine and harmaline from Peganum harmala. Fitoterapia, 66: 73–76. Munir C, Zaidi MI, Yousaf SM (1994). Zinc, cadmium and mercury as extractants of nicotine from tobacco leaves. Main Group Metal Chem., 17: 673–677. Pasaoglu H, Guven S, Heren Z, Buyukgungor O (2006). Synthesis, spectroscopic and structural investigation of ZnI2(nicotinamide)2, ZnI2(isonicotinamide)2 and [Zn(H2O)2(picolinamide)2]I2. J. Mol. Struct., 794: 270–276. Shen L, Zhang H, Ji HF (2007). Computational note on the SOD-like antioxidant potential of nicotine–copper(II) complexes. J. Mole. Struct., Theochem., 817: 161–162. Takeda A, Tamano H, Kan F, Itoh H, Oku N (2007). Anxiety-like behavior of young rats after 2-week zinc deprivation. Behav. Brain Res., 177: 1–6. Zaidi MI, Gul A (2005). Antibacterial activity of nicotine and its copper complex. J. Sc. Tech. Univ. Peshawar, 29: 45–49.
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Table of Contents: Volume 6 Number
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Table 1. Overview of the Soil prope
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exactly. MICROBIAL BIOMASS IN ORGAN
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Table 3. Advantages and disadvantag
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delays to cooling and wrapping on s
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pilus (tcp) that is a subtle of pol
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protein. DISCUSSION 70 kda 60 kda 5
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Percentage repellency (%) Percentag
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Fumigant toxicity of essential oils
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Table 1. Primers used for PCR and s
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composition in qnrB alleles. Althou
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conventional method. The reasons fo
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Figure 1. Comparison of amplificati
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Yokoyama E, Yamagishi K, Hara A (20
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a c Rex DNA Tang et al. 5235 Figure
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Table 1. Gender and division wise d
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62% were genotype D, A (14%), C (6%
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Swimming time (s) 1000 800 600 400
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the Central Universities (Grant No.
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prunin so far. By using the HPLC me
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