Research Publications - College of Medicine and Health Science

Department of Biochemistry
14
Baicalein inhibits α-syn oligomerization in living cells. Hela cells were transfected with GNS+SGC or EGPF plasmids then followed
by baicalein treatment for 48 hours. Baicalein dose-dependently inhibited co-expression of GNS+SGC-caused fluorescence signals,
but did not affect fluorescence signals caused by EGFP expression alone (A, B). Hela cells and SH-SY5Y cells were transfected
with GNS+SGC then treated with different concentration of baicalein for 48 hours. Cell lysate was electrophoresed on Native
PAGE Novex 4–16% Bis-Tris gel or SDS PAGE gel and probed with anti-α-syn and anti-tubulin antibodies, respectively (C). P2
population in flow cytometry graphs indicates cells containing fluorescent α-syn oligomers. Results from the flow cytometry data
are presented as means ±S.E. of at least three independent experiments. *P