Research Publications - College of Medicine and Health Science
Research Publications - College of Medicine and Health Science
Research Publications - College of Medicine and Health Science
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Pam3CysSK4 induces neurodegeneration in the murine dentate gyrus.<br />
Merged TUNEL-Hoechst staining (A,B) reveals an increase in the number <strong>of</strong> apoptotic cells in the dentate gyrus in wildtype mice<br />
challenged with intrathecal Pam3CysSK4 (A) compared to controls receiving intrathecal PBS, but not in TLR2-/- mice (B). C:<br />
quantitative analysis <strong>of</strong> TUNEL positive nuclei in the dentate gyrus per area on multiple sections per animal. *, p < 0.05; One-way<br />
ANOVA followed by Student-Newman-Keuls post-hoc analysis. Published <strong>and</strong> provided by Pr<strong>of</strong>. Braun.<br />
Cell type specific expression <strong>of</strong> TLR2 in the CNS.<br />
A: Real time PCR using GAPDH (hatched bars) <strong>and</strong><br />
-Actin (closed bars) as internal st<strong>and</strong>ards reveals<br />
very low presence <strong>of</strong> TLR2 mRNA in neurons <strong>and</strong><br />
astrocytes, but high levels in microglia. AU; arbitrary<br />
units. B-G: Immunocytochemical localisation <strong>of</strong> TLR2<br />
(C,F). Murine primary microglia (B-D) is identified by<br />
IB4 immunostaining (B); neurons (E-G) are identified by<br />
MAP-2 immunoreactivity (E). D,G: merged images.<br />
Bar = 20 μm. Published <strong>and</strong> provided by Pr<strong>of</strong>. Braun.<br />
Department <strong>of</strong> Internal <strong>Medicine</strong><br />
37<br />
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