sodininkystÄ ir darÅ¾ininkystÄ 31(3â4)

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81. van den Berg R., Haenen G. R. M. M., van den Berg H., Bast A. 1999.Applicability of an improved Trolox equivalent antioxidant capacity (TEAC)assay for evaluation of antioxidant capacity measurements of mixtures. FoodChem., 66: 511–517.82. Wojdylo A., Oszmianski J., Czemerys R. 2007. Antioxidant activity and phenoliccompounds in 32 selected herbs. Food Chem., 105: 940–949.83. Wolfe K. L., Liu R. H. 2007. Cellular antioxidant activity (CAA) assay for assessingantioxidants, foods, and dietary supplements. J. Agric. Food Chem., 55:8 896–8 907.84. Wootton-Beard P. C., Moran A., Ryan L. 2011. Stability of the total antioxidantcapacity and total polyphenol content of 23 commercially available vegetablejuices before and after in vitro digestion measured by FRAP, DPPH, ABTS andFolin–Ciocalteu methods. Food Res. Int., 44: 217–224.85. Wright J. S., Johnson E. R., DiLabio G. A. 2001. Predicting the activity of phenolicantioxidants: theoretical method, analysis of substituent effects, and applicationto major families of antioxidants. J. Am. Chem. Soc., 123: 1 173–1 183.86. Zhang Q., van der Klift E. J. C., Janssen H-G., van Beek T. A. 2009. An on-linenormal-phase high performance liquid chromatography method for the rapiddetection of radical scavengers in non-polar food matrixes. J. Chromatogr. A, 1216: 7 268–7 274.SODININKYSTĖ IR DARŽININKYSTĖ. SCIENTIFIC ARTICLES. 2012. 31(3–4).Assessment of the Antiradical and Reducing activity (Review)R. Raudonis, L. Raudonė, V. Janulis, P. ViškelisSummaryAntioxidant is a substance that effectively reduces pro-oxidant resulting in compoundswith low or no toxicity. Interest in protective properties of natural antioxidants of plant originand their application in prophylaxis is increasing. Preliminary researches on antioxidant activityof herbal raw materials are carried out by in vitro methods. The most popular methods based onsingle electron transfer (SET) are assessment of DPPH radical, ABTS radical-cation scavenging,ferric (FRAP) and cupric (CUPRAC) ion reducing antioxidant power. The most commonly,detection of change in spectrophotometric visual light absorption is used. It’s a simple, reliableand suitable method for evaluation of antioxidant properties of pure compounds and foridentification of structure-activity relationship. In complex mixtures such as plant extracts andfood matrixes spectrophotometric methods evaluate total antioxidant activity of all compoundspresent in a sample and cannot evaluate the contribution of separate compounds to overallactivity. High-resolution online assays are used for a rapid separation of active antioxidantcompounds. These assays combine HPLC separation and post-column reaction detection. HPLCpost-column assays assess antioxidant properties of separate compounds and their contributionto the overall antioxidant activity of complex mixtures. HPLC post-column assays provide reliable,accurate and reproducible assessment of quality of plant extracts by determining markersof qualitative and quantitative antioxidant activity of biologically active compounds. Assays34
enable the control of distribution and stability of antioxidants and determination of optimalstorage conditions of materials or preparations. HPLC post-column assays are effective meansfor plant selection among different species, varieties, phenological stage and growing conditionsin order to ensure abundant antioxidant composition.Key words: ABTS, antioxidants, CUPRAC, DPPH, FRAP, HPLC post-column.35
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easons of the study, innovation. Sh
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ISSN 0236-4212Mokslinis leidinysLie
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