The Principles of Clinical Cytogenetics - Extra Materials - Springer

484 Daynna Wolff and Stuart Schwartz
Fig. 20. Multicolor banding: (A) region-specific probes labeled with different partial chromosome paints
(PCP) and computer false color (MetaSystems’ mBAND) produces a definable number of colored bands per
chromosome, regardless of chromosome length; (B) this example shows an abnormal X chromosome (right
homolog of each pair). Using GTG-banding, the chromosome was initially diagnosed as a paracentric inversion
of the long arm (left). mBAND analysis, however, suggests an isodicentric chromosome X (center). Note the
identical band colors in both chromosome arms. An X centromere probe supports this interpretation (right).
(Courtesy of MetaSystems Group, Inc.)
Fig. 21. Primed random in situ hybridization (PRINS). Metaphase chromosomes are subjected to PRINS
with α-satellite oligonucleotides specific for chromosomes X, 11, and 17. Bright yellow fluorescein staining is
seen at the centromeres of these chromosomes. See text for details. (Courtesy of Drs. Steen Kolvraa and Lars
Bolund, Aarhus University, Sweden.)