Inhibitor SourceBook™ Second Edition

Proteases
Calpain Inhibitors, continued
Product Cat. No. Comments Size Price
EST 330005 [E-64d; (2S,3S)-trans-Epoxysuccinyl-L-leucylamido-3-methylbutane Ethyl
Ester; Loxistatin]
A cell-permeable, irreversible inhibitor of cysteine proteases that is
reported to block the action of calpain I. Its action is similar to E-64
(Cat. No. 324890); however, it is devoid of charged groups.
mg $96
PD 45305 513021 A useful negative control for the calpain inhibitors PD 50606
(Cat. No. 5 3022) and PD 5 746 (Cat. No. 5 3024).
mg $99
5 mg $94
PD 50606 513022 [3-(4-Iodophenyl)-2-mercapto-(Z)-2-propenoic Acid]
A cell-permeable, selective non-peptide calpain inhibitor (K i = 2 0 nM
for calpain I and 370 nM for calpain II) directed towards the calcium
binding sites of calpain.
PD 5 746 513024 [3-(5-Fluoro-3-indolyl)-2-mercapto-(Z)-2-propenoic Acid]
A cell-permeable, non-peptidic, and highly selective calpain inhibitor
that displays over 20-fold greater selectivity for calpain I (K = 260 nM)
i
over calpain II (K = 5.33 mM).
i
Calpain Inhibitor Set 208733 Provided as a 5 vial set. Each set contains 5 mg ALLN (Cat. No. 2087 9);
25 mg Calpain Inhibitor III (Cat. No. 208722); 5 mg of Calpeptin (Cat.
No. 03-34-005 ); mg of EST (Cat. No. 330005); and 5 mg of PD
50606 (Cat. No. 5 3022).
Related Products
Calpain-1 Substrate II, Fluorogenic
An internally quenched peptide substrate that is optimized for calpainamino
acid recognition motifs at the primed as well as the nonprimed
sides.
Cat. No. 208772 1 mg $180
Calpain Substrate III, Fluorogenic
A fluorogenic FRET peptide with a substrate sequence that is optimized
for calpain- and -2 (Cat. Nos. 2087 2, 2087 3, 2087 5, and 2087 8)
based on 06 known cleavage sites. It is cleaved by mammalian
calpains with a much better efficacy (k cat /K mx 0 -2 M - s - ) = 69.8,
38.5, 20.0, 6.8 , 3.58, 0.05, and < 0.05 for calpain-2, trypsin, papain,
calpain-B, calpain-A, chymotrypsin, and cathepsin-B, respectively)
and kinetically superior to other commonly used calpain substrates,
such as LY-AMC (Cat. No. 20873 ) and a-spectrin cleavage site-based
substrate (Cat. No. 208748). Has been used successfully in monitoring
calpain activity in whole Drosophila S2 cells (cellular incorporation
achieved with a lipofection reagent) and in COS-7 cell lysate.
Purity: ≥98% by HPLC.
Cat. No. 208771 1 mg $195
Calpain Activity Assay Kit, Fluorogenic
Assay kit useful for the quantitative determination of calpain- and
calpain-2 activity in human cell lysates, plasma, and serum and for
screening calpain inhibitors. Requires a fluorimeter or microplate
reader capable of measuring fluorescence at wavelengths of
Ex. max: ~360 nm and Em. max: ~460 nm.
Cat. No. QIA120 1 kit $345
Calpain Substrate II, Fluorogenic
Cyclic fluorogenic substrate for the quantitative determination of
calpain - and -2. Also suitable for measuring the peptidase activity of
the 20S proteasome. Typical concentrations range from 0– 00 µM,
depending on assay conditions. Purity: ≥95% by TLC. Ex. max.: ~380 nm,
Em. max.: ~460 nm.
Cat. No. 208731 25 mg $75
Calpain-1 Substrate, Fluorogenic
2 mg $ 69
set $325
An internally quenched fluorogenic substrate peptide derived from the
calpain- cleavage site of a-spectrin. It is not recognized by trypsin
or a-chymotrypsin and serves as a sensitive and specific substrate for
calpain- (K m = 4.6 µM; k cat = s - ). Cleavage occurs between Tyr-Gly
residues and results in enhanced fluorescence. Purity: ≥95% by HPLC.
Ex. max.: ~490 nm, Em. max.: ~518 nm.
Cat. No. 208748 2 mg $163
View more than 20 anti-calpain
antibodies at our updated
Antibody Resource
www.calbiochem.com/
antibodyresource
6 Orders Phone 800 854 34 7
Calbiochem • Inhibitor SourceBook
Fax 800 776 0999
Web www.emdbiosciences.com/calbiochem