Inhibitor SourceBook™ Second Edition

Proteases
Protease Inhibitor Cocktails, continued
Protease Inhibitor Cocktail Set VII
This cocktail is recommended for purification of proteins containing His•Tag® sequences. Cocktail contains five protease
inhibitors (in ml DMSO) with broad specificity for the inhibition of cysteine, serine, aspartic, and thermolysinlike
proteases and aminopeptidases. One ml is recommended for the inhibition of proteases in 0 g cells.
Cat. No. 539138 1 ml $62
1 set (5 x 1 ml) $267
Protease Inhibitor Cat. No. Mol. Wt. Concentration in the Vial Target Protease
AEBSF, Hydrochloride 101500 239.5 00 mM Serine Proteases
Bestatin 200484 308.4 5 mM Aminopeptidase B and Leucine
Aminopeptidase
E-64 Protease Inhibitor 324890 357.4 .5 mM Cysteine Proteases
Pepstatin A 516482 685.9 2 mM Aspartic Proteases
Phosphoramidon 525276 587.5 200 mM Metalloendopeptidases
Protease Inhibitor Cocktail Set VIII
A DMSO solution of three protease inhibitors with selective specificity for the inhibition of cysteine proteases,
including calpains, cathepsins, and papain.
Cat. No. 539129 1 ml $63
1 set (5 x 1 ml) $268
Protease Inhibitor Cat. No. Mol. Wt. Concentration in the Vial Target Protease
ALLN 208719 383.5 .56 mM Calpain I/II, Cathepsin B,
Cathepsin L, Cysteine Proteases
Cathepsin Inhibitor I 219415 475.5 500 mM Cathepsin B, Cathepsin L,
Cathepsin S, Papain
E-64 Protease Inhibitor 324890 357.4 .5 mM Cysteine Proteases
Protease Arrest Reagent
An optimized concentration of various reversible and irreversible
inhibitors to inhibit serine, cysteine, and calpain proteases. Suitable for
the protection of proteins purified from animal tissues, plant tissues,
yeast, and bacteria. Protease Arrest Reagent is provided as a 50x
solution that when diluted in extraction buffer at pH 7.0 to 8.0 inhibits
95-98% of protease activity. EDTA is also provided separately to inhibit
metalloproteinases.
Cat. No. 539124 1 set $150
Protease Inhibitor Set
A set of 6 vials. Each set contains 50 mg of AEBSF, HCl (Cat. No.
0 500), mg of E-64 (Cat. No. 324890), mg of EST (E-64d; Cat. No.
330005), 5 mg of Leupeptin, Hemisulfate (Cat. No. 08975), 5 mg of
Pepstatin A (Cat. No. 5 6482), 50 mg of TLCK, HCl (Cat. No. 6 6382),
and 250 mg of TPCK (Cat. No. 6 6387).
Cat. No. 539128 1 set $274
Serine Protease Inhibitor Cocktail Set I
A cocktail of four protease inhibitors that is useful for inhibition of a
broad range of serine proteases. Reconstitute each vial with ml of
H 2 O to obtain a 00x stock solution. x stock solution contains
500 mM AEBSF, HCl (Cat. No. 0 500), 420 nM Aprotinin (Cat. No.
6 6370), 20 mM Elastatinal (Cat. No. 32469 ), and mM GGACK (Cat.
No. 347436).
Cat. No. 565000 1 vial
5 vials
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$60
$239