effect of infection of the filarial parasite brugia malayi - Pondicherry ...

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4. MTT : 5 mg in 1 ml water 5. Phenazine metho suiphate (PMS) : 2.5 rng in 0.5 ml water 6. Agar (approx., 2%) :20ml . Electrophoresis conditions 1. Bridge buffer: 0.2 M phosphate buffer, pH 7.0 Gel buffer : 0.01 M phosphate buffer. pH 7.0 2. I2 Vlcm for 5 b, with cooling. One percent agarose gel was prepared in 0.01 M phosphate buffer and fvted on a horizontal electrophoretic system. About 10 p1 of each sample were added into individual well along with the control sample. Whatman filter paper was used as the wick between the bridge buffer and electrophoresis was carried out at constant current (I2 V) for 5 hrs. The isoenzymes were visualised by activity staining as follows. The unfixed gel was rinsed with cold his buffer (0.1 M, pH 8.3) and incubated for 10 minutes at 37C in dark in a staining solution containing 200 p1 calcium lactate, 10 mg NAD, 5 mglml m, 2.5 md0.5 ml PMS in 2% agar made upto 25 ml. The isoenzymes LDH 1-4 migrated anodally, while LDH-5 migrated cathodally. The LDH-X isoenzyme migrates between LDH-3 and LDH 4. 43.2. RESULTS The effect of B. ma lay^ on testosterone, y -GTP and LDH-X of testes was studied and the data are presented in Figs. 114 8~115. The testosterone levels were observed to increase initially (upto 60 days) and decreased significantly w0.05) thereafter. And althrough the study period. the activity of this hormone was always lower than the control. y-GTP level increased significantly WO.05) in the infected group during the parasite development than in control throughout the study period. The LDH-X mzyme was visualized on agarose gel by activity staining (Platel). The LDH-X bands in the infected animals were clearly visible on 30th day and faintly visible on 60th day but absenthot visible on 90th and 120th day, whereas. in control the enzyme bands were clearly visible throughout the study period. 9 9
Fig.114: Effect of B, malayi on GTP testes of M. natalensis Days of infection - - gamma GTP-N gamma GTP-I Fig.115: Effect of 8. malayi on Testosterone in testes of M. natalensis 104 0 30 60 90 1 Days of infection
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EFFECT OF INFECTION OF THE FILARIAL
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I wish to express my deep sense of
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1. INTRODUCTlON 2. REVIEW OF LlTERA
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As stated earlier, the host immune
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13 Testicular changes Testosterone
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2. REVIEW OF LITERATURE Filariasis
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2.2 Clinical signs Inflammalation o
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the tegument by the release of thei
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the compound kills the filarial wor
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3. MATERIALS AND METHODS COLONY MAI
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Ragenb 1. Tris-HCI buffer . : 0.1 M
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3. Reduced glutathione : 30 mM To I
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4.1.1.6 Glucose-6-phosphate dehydro
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absorbance of the clear supernatant
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-eats I. Lowry's reagent :Solution
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4.1.2.1 Changes in antioxidant enzy
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Fig.3: Effect of B.malayi on SOD in
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Fig.9: Effect of B.rnalayi on GST i
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Fig.12: Effect of 6.rnalayi on GPx
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Fig. 18: Effect of B.malayi on G6PD
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12- 3 m- 15- lor, Fig.21: Effect of
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Fig.27: Effect of B.malayi on Ascor
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4.1.2.3 Change in the antioxidant e
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Fig.35: Effect of B.malayi on CAT i
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m 20 0) - 15 9 g 10 il! 5 0 Fig.39:
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30 25 8 - a 20 2 15 C I 10 I 5 0 35
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Fig.45: Effect of B.maiayi on GSH i
Page 59 and 60: Fig.49: Effect of B.malayi on Vit-C
Page 61 and 62: 4.1.3 DISCUSSION 4.1 3.1.1 Toxic ox
Page 63 and 64: 1973). The two major systems of LPO
Page 65 and 66: 1981), and their increase in human
Page 67 and 68: 1 0 Fig.58:Relationship between cha
Page 69 and 70: 1 - 0- ' 1 5 - 6 - 7 - Fig.63:Relat
Page 71 and 72: Catalase had been reported to be re
Page 73 and 74: - Fig.68:Relationship between chang
Page 75 and 76: esults in its reduced activity (Con
Page 77 and 78: g 20- :I-. F1g.70:Relationship betw
Page 79 and 80: 12, - I 0 U) Q 4 2 0 - + - Fig.75:R
Page 81 and 82: $ Fig.80:Relationship between chang
Page 83 and 84: Fig.85:Relationship between change
Page 85 and 86: V) (3 :; - Fig.9O:Relationship betw
Page 87 and 88: A reduction in the total thiol cont
Page 89 and 90: 2 5- Fig.98:Relationship between ch
Page 91 and 92: Fig. 103:Relationship between chang
Page 93 and 94: 4.2.1.1 N ay ATPase Reagents Nay AT
Page 95 and 96: Reagents 1. Acetylcholine bromide :
Page 97 and 98: To 1.5 ml of carbonate buffer, 1.5
Page 99 and 100: 4.2.1.10 Differential leucocyte cou
Page 101 and 102: increase in the control animals (Fi
Page 103 and 104: - Fig.110: Effect of 6. malayi on H
Page 105 and 106: efflux and thereby alter the membra
Page 107 and 108: with i n d lymphocytes and decrease
Page 109: Tubes were labeled for calibrators,
Page 113 and 114: 433 DISCUSSION Testosterone is a se
Page 115 and 116: CHAPTER 4.4 HISTOPATHOLOGICAL CHANG
Page 117 and 118: Platc 2 Plate 3 Plate 4 Plate 5 Pla
Page 119 and 120: Platc X Plate 9 Plate 10 Plate 11 P
Page 121 and 122: Plate w: Section of Testes of norma
Page 123 and 124: 4.4.2.1.5 Brain The control animals
Page 126 and 127: Plate ICt Section of Kidney of infe
Page 128 and 129: 4.4.2.2 Histopathological changes i
Page 130 and 131: Plate 32
Page 132: The mf could not be seen in spleen
Page 135 and 136: ain, it decreased from 0 day itself
Page 137: inflammatory nature indicative of c
Page 141 and 142: BIBLIOGRAPHY Ackerman, S.J., Gleich
Page 143 and 144: Case, T., Leis, B., Witte, M., Way,
Page 145 and 146: Drabkin, D.L.R. and Austin, J.H. (1
Page 147 and 148: Fukuota, M., Zhou, Y. and Tanaka.(l
Page 149 and 150: Jacob, H.S. and Lux, S.E. (1968). D
Page 151 and 152: Linda, I. and Mayeda, B. (1974). Th
Page 153 and 154: Murray, H.W. (1981). Susceptibility
Page 155 and 156: Santiago- Stevenson, D., Oliver-Gon
Page 157 and 158: Tate, S.S., Thomson, G.A. and Meist
Page 159 and 160: Zaini, M., Ramachandran, C.P. and E
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