effect of infection of the filarial parasite brugia malayi - Pondicherry ...

More documents

Recommendations

Info

Fig.51: Effect of B.malayi on LPO in Liver & Testis after DEC treatment Organ I Control Control+D Infected Infected+ D 1 12 g 10 - 3 m 8 > 5 2 2 Fig.52: Effect of B.malayi on LPO in Brain,Heart & Lungs after DEC treament 0 Organ Lungs
4.1.3 DISCUSSION 4.1 3.1.1 Toxic oxygen radicals The last few decades have seen great advances in understanding the parasite induced host physiology in t m of host defense mechanism especially the role of oxidants (free radicals) and antioxidant enzymes (Callahan et al., 1988). A free radical is any species capable of independent existence that contains one or more unpaired electrons. They are highly reactive and unstable and can injure some biological targets such as proteins, lipids, carbohydrates and key molecules in membrane and nucleic acids. Moreover, free radicals initiate autocatalytic reaction whereby, molecules with which they react are themselves converted into free radical and thus propagate the chain of damage (Halliwell and Guneridge, 1989). The free radicals may be initiated within cells during the normal cellular metabolic processes (Klebanoff, 1974; 1980), by ionizing radiation and certain anti-parasitic drugs (Docampo and Moreno, 1984) and the host response is also a potent source of oxidants. Molecular oxygen is a buadical and a relatively unreactive compound. It undergoes a four- electron reduction to form water. However, it can be metabolized inviw to form highly reactive derivative oxidants. A series of sequential one-electron transfer yields three reactive intermediates superoxide anions, hydrogen peroxide and hydroxyl mhcal. When two free radicals meet, they can join their unpaired electrons to form a covalent bond. When radicals react with non-radicals. new radicals are generated invivo, which is likely to set off free radical chain reaction (Halliwell et al., 1995; Thomas, 1995) (Fig. 53). The best studied biologically relevant fra radical chain reaction is lipid peroxidation. 4.13.1.2 Lipid peroddation Lipid peroxidation is an important consequence of oxidative cellular damage (Plea and Witschi, 1976) involved in a variety of diseases and stress (Slater, 1984; Halliwcll and Guttnidge, 1989). LPO involves the direct reaction of oxygen and ply unsaturated fatty acid (PUFA) to form fm radicals and semistable -ides (Tappel,
Page 1 and 2:
EFFECT OF INFECTION OF THE FILARIAL
Page 4 and 5:
I wish to express my deep sense of
Page 6:
1. INTRODUCTlON 2. REVIEW OF LlTERA
Page 9 and 10: As stated earlier, the host immune
Page 11 and 12: 13 Testicular changes Testosterone
Page 14 and 15: 2. REVIEW OF LITERATURE Filariasis
Page 16 and 17: 2.2 Clinical signs Inflammalation o
Page 18 and 19: the tegument by the release of thei
Page 20 and 21: the compound kills the filarial wor
Page 22: 3. MATERIALS AND METHODS COLONY MAI
Page 25 and 26: Ragenb 1. Tris-HCI buffer . : 0.1 M
Page 27 and 28: 3. Reduced glutathione : 30 mM To I
Page 29 and 30: 4.1.1.6 Glucose-6-phosphate dehydro
Page 31 and 32: absorbance of the clear supernatant
Page 33 and 34: -eats I. Lowry's reagent :Solution
Page 35 and 36: 4.1.2.1 Changes in antioxidant enzy
Page 37 and 38: Fig.3: Effect of B.malayi on SOD in
Page 39 and 40: Fig.9: Effect of B.rnalayi on GST i
Page 41 and 42: Fig.12: Effect of 6.rnalayi on GPx
Page 43 and 44: Fig. 18: Effect of B.malayi on G6PD
Page 45 and 46: 12- 3 m- 15- lor, Fig.21: Effect of
Page 47 and 48: Fig.27: Effect of B.malayi on Ascor
Page 49 and 50: 4.1.2.3 Change in the antioxidant e
Page 51 and 52: Fig.35: Effect of B.malayi on CAT i
Page 53 and 54: m 20 0) - 15 9 g 10 il! 5 0 Fig.39:
Page 55 and 56: 30 25 8 - a 20 2 15 C I 10 I 5 0 35
Page 57 and 58: Fig.45: Effect of B.maiayi on GSH i
Page 59: Fig.49: Effect of B.malayi on Vit-C
Page 63 and 64: 1973). The two major systems of LPO
Page 65 and 66: 1981), and their increase in human
Page 67 and 68: 1 0 Fig.58:Relationship between cha
Page 69 and 70: 1 - 0- ' 1 5 - 6 - 7 - Fig.63:Relat
Page 71 and 72: Catalase had been reported to be re
Page 73 and 74: - Fig.68:Relationship between chang
Page 75 and 76: esults in its reduced activity (Con
Page 77 and 78: g 20- :I-. F1g.70:Relationship betw
Page 79 and 80: 12, - I 0 U) Q 4 2 0 - + - Fig.75:R
Page 81 and 82: $ Fig.80:Relationship between chang
Page 83 and 84: Fig.85:Relationship between change
Page 85 and 86: V) (3 :; - Fig.9O:Relationship betw
Page 87 and 88: A reduction in the total thiol cont
Page 89 and 90: 2 5- Fig.98:Relationship between ch
Page 91 and 92: Fig. 103:Relationship between chang
Page 93 and 94: 4.2.1.1 N ay ATPase Reagents Nay AT
Page 95 and 96: Reagents 1. Acetylcholine bromide :
Page 97 and 98: To 1.5 ml of carbonate buffer, 1.5
Page 99 and 100: 4.2.1.10 Differential leucocyte cou
Page 101 and 102: increase in the control animals (Fi
Page 103 and 104: - Fig.110: Effect of 6. malayi on H
Page 105 and 106: efflux and thereby alter the membra
Page 107 and 108: with i n d lymphocytes and decrease
Page 109 and 110: Tubes were labeled for calibrators,
Page 111 and 112:
Fig.114: Effect of B, malayi on GTP
Page 113 and 114:
433 DISCUSSION Testosterone is a se
Page 115 and 116:
CHAPTER 4.4 HISTOPATHOLOGICAL CHANG
Page 117 and 118:
Platc 2 Plate 3 Plate 4 Plate 5 Pla
Page 119 and 120:
Platc X Plate 9 Plate 10 Plate 11 P
Page 121 and 122:
Plate w: Section of Testes of norma
Page 123 and 124:
4.4.2.1.5 Brain The control animals
Page 126 and 127:
Plate ICt Section of Kidney of infe
Page 128 and 129:
4.4.2.2 Histopathological changes i
Page 130 and 131:
Plate 32
Page 132:
The mf could not be seen in spleen
Page 135 and 136:
ain, it decreased from 0 day itself
Page 137:
inflammatory nature indicative of c
Page 141 and 142:
BIBLIOGRAPHY Ackerman, S.J., Gleich
Page 143 and 144:
Case, T., Leis, B., Witte, M., Way,
Page 145 and 146:
Drabkin, D.L.R. and Austin, J.H. (1
Page 147 and 148:
Fukuota, M., Zhou, Y. and Tanaka.(l
Page 149 and 150:
Jacob, H.S. and Lux, S.E. (1968). D
Page 151 and 152:
Linda, I. and Mayeda, B. (1974). Th
Page 153 and 154:
Murray, H.W. (1981). Susceptibility
Page 155 and 156:
Santiago- Stevenson, D., Oliver-Gon
Page 157 and 158:
Tate, S.S., Thomson, G.A. and Meist
Page 159 and 160:
Zaini, M., Ramachandran, C.P. and E
show all

effect of infection of the filarial parasite brugia malayi - Pondicherry ...

You also want an ePaper? Increase the reach of your titles

Delete template?

Save as template?