effect of infection of the filarial parasite brugia malayi - Pondicherry ...

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4.4.2.2 Histopathological changes in relation to DEC treatment All the organs of the infected DEC treated animals showed similar histological changes like that of the infected untreated animals. However, the testicular tissue showed severe degeneration and the seminiferous tubules were completely devoid of spermatogenic cells and spermatozoa (Plate 32). They contained only fibrous material. In some areas, moderate to severe infiltration of the lymphocytes, plasma cells and macrophages were recorded. Adult female parasite sections were present in pockets (Plate 33). In some sections calcified adult parasites were observed. Mild connective tissue proliferation was present around the parasite in some sections. 4.4.3. DISCUSSION Vincent et al. (1976) studied the chronological development of pulmonary pathology associated with B. malayi, B. pahangi and B. patei in Meriones unguiculatus, which caused inflammatory reactions. Pulmonary granulomas were observed during the final molt, followed by involution and formation of residual vascular lesions and some were seen during sexual maturity of the worms. Obstructive endarteritis and chronic interstitial inflammation with degenerating mf were also observed. Malone et al., (1976) studied the histopathological lesions in the lymphatic system and other major organs of hamsters infected with B. pahangi. Cellular infiltration of plasma cells and eosinophil, obstruction of pulmonary arteries and obstructive granulomatous lymphangitis were observed. Live and dead worms were found in testicular parenchyma. Accumulation of eosinophils, large mononuclear cells and plasma cells were seen in interstitial tissues between superfacial seminiferous tubules. Heavy accumulation of hemosiderin and giant cells were also observed in the lung. Degenerative or necrotic hepatocytes occurred In the liver. Schacher and Sahyoun (1967) reported pathologic changes due to B. pahangi in experimentally infected cats and dogs. Hyperplasia of lymph follicles and reticular cells of the nodal stroma had been reported (Schacher and Sahyoun, 1967; Mak, 1983). Destruction of mf had-been observed in the spleen, which caused acute and chronic inflammatory reaction in patients infected with B. malayi (Mak, 1983). Large number of lesions were observed in liver, lungs and spleen of ferret infected with B, malayi and B.
Plate 31: Section of Testes of infected DEC treated group showing severe testicular degeneration and C.S. of adult parasite (Haernatoxylin and Eosin IOOX). ' Plate Section of Testes of infected DEC treated group showing sections of adult parasite (Haematoxylin and Eosin IOOX).
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EFFECT OF INFECTION OF THE FILARIAL
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I wish to express my deep sense of
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1. INTRODUCTlON 2. REVIEW OF LlTERA
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As stated earlier, the host immune
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13 Testicular changes Testosterone
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2. REVIEW OF LITERATURE Filariasis
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2.2 Clinical signs Inflammalation o
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the tegument by the release of thei
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the compound kills the filarial wor
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3. MATERIALS AND METHODS COLONY MAI
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Ragenb 1. Tris-HCI buffer . : 0.1 M
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3. Reduced glutathione : 30 mM To I
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4.1.1.6 Glucose-6-phosphate dehydro
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absorbance of the clear supernatant
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-eats I. Lowry's reagent :Solution
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4.1.2.1 Changes in antioxidant enzy
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Fig.3: Effect of B.malayi on SOD in
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Fig.9: Effect of B.rnalayi on GST i
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Fig.12: Effect of 6.rnalayi on GPx
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Fig. 18: Effect of B.malayi on G6PD
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12- 3 m- 15- lor, Fig.21: Effect of
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Fig.27: Effect of B.malayi on Ascor
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4.1.2.3 Change in the antioxidant e
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Fig.35: Effect of B.malayi on CAT i
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m 20 0) - 15 9 g 10 il! 5 0 Fig.39:
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30 25 8 - a 20 2 15 C I 10 I 5 0 35
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Fig.45: Effect of B.maiayi on GSH i
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Fig.49: Effect of B.malayi on Vit-C
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4.1.3 DISCUSSION 4.1 3.1.1 Toxic ox
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1973). The two major systems of LPO
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1981), and their increase in human
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1 0 Fig.58:Relationship between cha
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1 - 0- ' 1 5 - 6 - 7 - Fig.63:Relat
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Catalase had been reported to be re
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- Fig.68:Relationship between chang
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esults in its reduced activity (Con
Page 77 and 78: g 20- :I-. F1g.70:Relationship betw
Page 79 and 80: 12, - I 0 U) Q 4 2 0 - + - Fig.75:R
Page 81 and 82: $ Fig.80:Relationship between chang
Page 83 and 84: Fig.85:Relationship between change
Page 85 and 86: V) (3 :; - Fig.9O:Relationship betw
Page 87 and 88: A reduction in the total thiol cont
Page 89 and 90: 2 5- Fig.98:Relationship between ch
Page 91 and 92: Fig. 103:Relationship between chang
Page 93 and 94: 4.2.1.1 N ay ATPase Reagents Nay AT
Page 95 and 96: Reagents 1. Acetylcholine bromide :
Page 97 and 98: To 1.5 ml of carbonate buffer, 1.5
Page 99 and 100: 4.2.1.10 Differential leucocyte cou
Page 101 and 102: increase in the control animals (Fi
Page 103 and 104: - Fig.110: Effect of 6. malayi on H
Page 105 and 106: efflux and thereby alter the membra
Page 107 and 108: with i n d lymphocytes and decrease
Page 109 and 110: Tubes were labeled for calibrators,
Page 111 and 112: Fig.114: Effect of B, malayi on GTP
Page 113 and 114: 433 DISCUSSION Testosterone is a se
Page 115 and 116: CHAPTER 4.4 HISTOPATHOLOGICAL CHANG
Page 117 and 118: Platc 2 Plate 3 Plate 4 Plate 5 Pla
Page 119 and 120: Platc X Plate 9 Plate 10 Plate 11 P
Page 121 and 122: Plate w: Section of Testes of norma
Page 123 and 124: 4.4.2.1.5 Brain The control animals
Page 126 and 127: Plate ICt Section of Kidney of infe
Page 130 and 131: Plate 32
Page 132: The mf could not be seen in spleen
Page 135 and 136: ain, it decreased from 0 day itself
Page 137: inflammatory nature indicative of c
Page 141 and 142: BIBLIOGRAPHY Ackerman, S.J., Gleich
Page 143 and 144: Case, T., Leis, B., Witte, M., Way,
Page 145 and 146: Drabkin, D.L.R. and Austin, J.H. (1
Page 147 and 148: Fukuota, M., Zhou, Y. and Tanaka.(l
Page 149 and 150: Jacob, H.S. and Lux, S.E. (1968). D
Page 151 and 152: Linda, I. and Mayeda, B. (1974). Th
Page 153 and 154: Murray, H.W. (1981). Susceptibility
Page 155 and 156: Santiago- Stevenson, D., Oliver-Gon
Page 157 and 158: Tate, S.S., Thomson, G.A. and Meist
Page 159 and 160: Zaini, M., Ramachandran, C.P. and E
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