effect of infection of the filarial parasite brugia malayi - Pondicherry ...

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6. CONCLUSION B. malayi infection of M. natalensis has brought about the following changes in different organs compared to healthy (control) animals: Decrease in the level of antioxidant enzymes and antioxidants in liver, testes, brain and heart, compared to an increase in lungs. Increase in the LPO of liver, testes, brain and heart, compared to a decrease in the lungs. All these shifts get restored to normal level (as in control animals) following the administration of DEC in all the organs except in testes. Haematological parameters and the level of membrane bound enzymes of the brain and RBC's get affected. Testicular damage occurs as evidenced by altered marker enzymes. Histopathological changes take place in liver, brain, testes, heart, lungs, kidney and spleen which do not get rectified even after DEC treatment, and in the case of testes the histopathological changes became more pronounced. Thus, it can be said that the host, M. natalensis responded to B. malayi infection, by damaging its own antioxidant defence system and consequently causing tissue injury in all the organs studied. However, in lungs the antioxidant defence system seems tc be less affected leading to reduced peroxidation of lipids. DEC treatment to infected animals restores the activity of antioxidant enzymes, but does not rectify the histopathological changes in all the organs and in fact, in testes, the tissue damage becomes more severe i.e., degeneration of seminiferous tubules leading to absence of spermatogenesis. Histopathological changes in various organs include mechanical injury caused by the parasite circulation and ischemia and hypoxia due to parasitic embolism. The degree of the pathological changes varied from organ to organ.
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EFFECT OF INFECTION OF THE FILARIAL
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I wish to express my deep sense of
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1. INTRODUCTlON 2. REVIEW OF LlTERA
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As stated earlier, the host immune
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13 Testicular changes Testosterone
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2. REVIEW OF LITERATURE Filariasis
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2.2 Clinical signs Inflammalation o
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the tegument by the release of thei
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the compound kills the filarial wor
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3. MATERIALS AND METHODS COLONY MAI
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Ragenb 1. Tris-HCI buffer . : 0.1 M
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3. Reduced glutathione : 30 mM To I
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4.1.1.6 Glucose-6-phosphate dehydro
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absorbance of the clear supernatant
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-eats I. Lowry's reagent :Solution
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4.1.2.1 Changes in antioxidant enzy
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Fig.3: Effect of B.malayi on SOD in
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Fig.9: Effect of B.rnalayi on GST i
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Fig.12: Effect of 6.rnalayi on GPx
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Fig. 18: Effect of B.malayi on G6PD
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12- 3 m- 15- lor, Fig.21: Effect of
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Fig.27: Effect of B.malayi on Ascor
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4.1.2.3 Change in the antioxidant e
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Fig.35: Effect of B.malayi on CAT i
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m 20 0) - 15 9 g 10 il! 5 0 Fig.39:
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30 25 8 - a 20 2 15 C I 10 I 5 0 35
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Fig.45: Effect of B.maiayi on GSH i
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Fig.49: Effect of B.malayi on Vit-C
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4.1.3 DISCUSSION 4.1 3.1.1 Toxic ox
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1973). The two major systems of LPO
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1981), and their increase in human
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1 0 Fig.58:Relationship between cha
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1 - 0- ' 1 5 - 6 - 7 - Fig.63:Relat
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Catalase had been reported to be re
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- Fig.68:Relationship between chang
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esults in its reduced activity (Con
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g 20- :I-. F1g.70:Relationship betw
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12, - I 0 U) Q 4 2 0 - + - Fig.75:R
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$ Fig.80:Relationship between chang
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Fig.85:Relationship between change
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V) (3 :; - Fig.9O:Relationship betw
Page 87 and 88: A reduction in the total thiol cont
Page 89 and 90: 2 5- Fig.98:Relationship between ch
Page 91 and 92: Fig. 103:Relationship between chang
Page 93 and 94: 4.2.1.1 N ay ATPase Reagents Nay AT
Page 95 and 96: Reagents 1. Acetylcholine bromide :
Page 97 and 98: To 1.5 ml of carbonate buffer, 1.5
Page 99 and 100: 4.2.1.10 Differential leucocyte cou
Page 101 and 102: increase in the control animals (Fi
Page 103 and 104: - Fig.110: Effect of 6. malayi on H
Page 105 and 106: efflux and thereby alter the membra
Page 107 and 108: with i n d lymphocytes and decrease
Page 109 and 110: Tubes were labeled for calibrators,
Page 111 and 112: Fig.114: Effect of B, malayi on GTP
Page 113 and 114: 433 DISCUSSION Testosterone is a se
Page 115 and 116: CHAPTER 4.4 HISTOPATHOLOGICAL CHANG
Page 117 and 118: Platc 2 Plate 3 Plate 4 Plate 5 Pla
Page 119 and 120: Platc X Plate 9 Plate 10 Plate 11 P
Page 121 and 122: Plate w: Section of Testes of norma
Page 123 and 124: 4.4.2.1.5 Brain The control animals
Page 126 and 127: Plate ICt Section of Kidney of infe
Page 128 and 129: 4.4.2.2 Histopathological changes i
Page 130 and 131: Plate 32
Page 132: The mf could not be seen in spleen
Page 135 and 136: ain, it decreased from 0 day itself
Page 137: inflammatory nature indicative of c
Page 142 and 143: Babior, B.M., Kipnes, R.S. and Curn
Page 144 and 145: Condell, R.A. and Tappel, A.L. (198
Page 146 and 147: Fairfield, AS., Abosch, A., Ram, A.
Page 148 and 149: Hams, H, and Hopkinson, D.A. (1978)
Page 150 and 151: Konigk, E. And Mirtsch, S. (1977).
Page 152 and 153: Marshall, G.R., Wickings, E.J. and
Page 154 and 155: Panicker, N.V. and Zyer, G.Y.N. (19
Page 156 and 157: Skinner, M.K. (1987). Cell-cell int
Page 158 and 159: Vincent, A.L., Frammes, S.P. and As
Page 160: APPENDIX Haematological values of M
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