effect of infection of the filarial parasite brugia malayi - Pondicherry ...

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I. Lowry's reagent :Solution A: 2% sodium carbonate in 0.1 N sod~um hydroxide solution.
prior to use.
: Solution B: 0.5% copper sulphate in 1% sodium potassium tartarate.
The reagent was prepared by mixing 50 ml of solution A with 1.0 ml of solution B
2. Folins Ciocalteau w ent
To 0.1 ml of (1:lO) diluted homogenate, 0.9 ml of water and 5.0 ml of Lowry's
reagent was added, mixed well and kept at RT. for 10 minutes. To this, 0.5 ml of Folin's
reagent was added and mixed.
Standard Bovine serum albumin solution containing 20-100 micrograms of protein
and a blank were treated in a similar manner. The intensity of the colour developed was
measured after 20 minutes at 660 nrn. The values were expressed as mg/p tissue.
4.1.2 RESULTS
The experimental animals w m necropsied on 0 day and on 30,60,90 and 120 days
post-inoculation with infective larvae of B. malayi for biochemical studies. On 120th day
post-inoculation, various organs were examined for the presence of adult worms. The worm
recovery was 25.1 5linfected animal and they were distributed in lungs, testes and heart and
liver and brain did not have adult worms. Lungs contained the maximum number of worms
followed by testes and heart (F1g.2).
Biochemical analyses were done to determine whether the B.malayi infection caused
any change in the production of oxygen €ree radicals, antioxidants and antioxidant enzymes
in different organs of the host animal, M. natalemis.