effect of infection of the filarial parasite brugia malayi - Pondicherry ...

4.2.1.10 Differential leucocyte count
(John, 1972).
Reagents
The leucocyte differential count was detenined by Leishman's staining method
1. Leishrnan's stain : 0.2 g of Leishman dye was dissolved in 100 ml of acetone free
methanol at 50°C for 15 minutes with occasional swng, then cooled and filtered.
2. Buffered water : 0.066 M Sotenson's phosphate buffer was made upto 50 ml, pH 7.0
and mixed with 3.89 ml of KH2P04 (9.1 gm4) and 61.1 ml NazHPO4 (9.5 gm4) to I litre
water
A drop of blood was placed on a clean slide and a spreading slide was placed at
an angle on the slide and moved forward to make a smear of 3-4 cm in length and air-dried.
The slide was covered with stain for 2-3 minutes and the stain was diluted by adding drops
of buffered water and left for 5-7 minutes. The slide was washed with water, dried and
examined under a microscope.
4.2.2 RESULTS
4.2.2.1 Membrane bound enzymes of brain.
The changes in the activity of the membrane bound e v e s of the brain are
presented in the Figs. 105-107. The activ~ty of all the enzymes studied ( Nay ATPase,
M ~ ATPase, ~ ' Ca2' ATPase, yGTP, acetylcholinesterase and alkaline phosphatase),
increased continuously from day 0 to day 120 in the control animals, whereas in the
B.malayi infected ones there was a significant (pC0.05) decrease from 60 days to 120 days.
43.2.2 Membrane bound enzymes of RBC
The activity N~'K' ATPase, Ca2' ATPase and h4g" ATPase in the infected animals
1- slightly from 0 day to 30 days and d e d the~fter as aglost the continous