university of kwazulu-natal faculty of science and agriculture school ...

a few cases (specifically stated in the experimental) all purifications were carried out on
2cm diameter columns collecting 50 ml fractions. Where 3cm and 1cm columns were
used, 100 ml fractions and 10 ml fractions were collected respectively. Any deviations to
these conditions are mentioned specifically in the experimental.
Infrared Spectroscopy (IR Spectroscopy)
Infrared spectra were recorded on a PerkinElmer TM Spectrum 100. The sample was
dissolved in either dichloromethane or methanol before being loaded onto the surface of
potassium bromide discs.
Ultraviolet Absorption Spectroscopy (UV)
Ultraviolet absorption spectra were recorded with a PerkinElmer TM Lambda 35 UV/VIS
spectrophotometer. Samples were dissolved in either dichloromethane or methanol and
made to concentrations between 0.0052g/100ml and 1.2g/100ml.
Optical Rotation
Optical rotations were performed with a PerkinElmer TM , Model 341 Polarimeter. The
samples were dissolved in dichloromethane or methanol and were measured at room
temperature. Concentrations were calculated in g/100mL.
Gas Chromatography-Mass Spectrometry (GC-MS)
The mass spectra were registered on an Agilent MS 5973 instrument which was coupled
to an Agilent GC 6890 instrument equipped with a DB-5MS (30m x 0.25 id, 0.25 µm
stationary phase) column. Helium (at 0.7ml/min) was used as the carrier gas. Optimal
results required that the oven temperature be maintained at 200 o C for one minute
followed by 5
98
o C increments in temperature every minute until an oven temperature of