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As with the total volume <strong>of</strong> sludge calculated with Eq. 5-3, this quantity only takes into account the<br />

solids content <strong>of</strong> the upflow side <strong>of</strong> each compartment and does not reflect the total mass <strong>of</strong> solids in<br />

the <strong>reactor</strong> and therefore cannot be used for mass balance purposes.<br />

Figure 5.18 shows that total solids accumulated in the upflow side <strong>of</strong> each compartment at a rate<br />

between 34 and 88 kg dry solids/year (Table 5.3).<br />

5.6.1.4 Rate <strong>of</strong> sludge accumulation: dependence on organic loading rate<br />

It was surprising that although the OLR in Phase III was double that <strong>of</strong> Phase IV, the Phase III sludge<br />

accumulation rate appears to be significantly more than double that <strong>of</strong> Phase IV. The ratio <strong>of</strong> the<br />

sludge accumulation rate in Phase III to that <strong>of</strong> Phase IV had a 95% confidence interval <strong>of</strong> 2.6 to 6.1<br />

(Table 5.3), calculated using Fieller’s theorem, Appendix A2.4) i.e. it can be said with 95%<br />

confidence that the rate <strong>of</strong> sludge accumulation in Phase III was between 2.6 and 6.1 times greater<br />

than that <strong>of</strong> Phase IV despite the fact that the OLR was approximately double.<br />

Table 5.4: Calculation <strong>of</strong> sludge accumulation rates normalised by organic loading rate for<br />

Phase III and Phase IV.<br />

Sludge accumulation rate Organic loading rate Normalised sludge accumulation<br />

rate<br />

Units m 3 sludge/year kg COD applied /<br />

m 3 <strong>reactor</strong> volume.year<br />

109<br />

ℓ sludge accumulated /<br />

(kg COD applied)<br />

Phase III 3.46 269 4.3<br />

Phase IV 0.901 146 2.1<br />

Phase IV<br />

(kg dry solids)<br />

60.7[kg dry solids/year] 146 0.14 [kg dry solids/kg COD applied]<br />

To explain this observation, the sludge accumulation rate was normalised using OLR 1 . Using average<br />

values only, the normalised sludge accumulation rates (i.e. amount <strong>of</strong> sludge accumulated per kg COD<br />

applied) were calculated and are presented in Table 5.4.<br />

Initially, it was expected that the normalised sludge accumulation rate would be higher in Phase IV<br />

than in Phase III since the lower feed flow rate resulted in lower upflow velocity in the upflow<br />

compartments, and this was expected to lead to better sludge retention. However,<br />

Table 5.4 shows a significantly lower normalised sludge accumulation rate in Phase IV than in Phase<br />

III.<br />

The most obvious explanation for the difference in normalised accumulation rates is that the additional<br />

solids accumulated in Phase III were undigested biodegradable particulate material originating from<br />

the feed material. The implication is that the resident <strong>anaerobic</strong> biomass population in the <strong>pilot</strong>-<strong>scale</strong><br />

ABR in Phase III was either not sufficiently concentrated or not sufficiently active to convert<br />

1 The sludge accumulation rate per organic loading quantity was calculated roughly for the purposes <strong>of</strong><br />

simplifying the calculations and therefore confidence intervals were not calculated.

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