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compartment 1 and 2, the increase was not sufficient to support the claim that phase separation<br />

occurred in the <strong>pilot</strong>-<strong>scale</strong> ABR where acidogenic reactions dominated in the first compartment, and<br />

methanogenic processes dominated in later compartments. It was clear that hydrolytic and acidogenic<br />

processes occurred in all compartments. However, spatial separation <strong>of</strong> compartments allowed regions<br />

characterised by slightly different pH values to develop, and this may have improved overall digestion<br />

rates and process stability (Section 6.1.1).<br />

7.1.1.4 Solids accumulation rate vs. feed flow rate<br />

Solids were observed to accumulate faster at an A-HRT <strong>of</strong> 22 h than at an A-HRT <strong>of</strong> 42 h. The solids<br />

accumulation rate per kg COD applied was also higher in the 22 h A-HRT period. Mass balance<br />

calculations indicated that approximately 30% <strong>of</strong> influent COD was removed as CH4 at the higher<br />

flow rate, while 60% was removed as CH4 at the lower flow rate. In addition, the free and saline<br />

ammonia concentration increase between inflow and outflow was greater at the lower flow rate. These<br />

data indicate that better digestion, termed extent <strong>of</strong> treatment <strong>of</strong> influent COD occurred at lower flow<br />

rates. A study using scanning electron microscopy confirmed this conclusion in that it observed low<br />

micro-organism concentrations, poor microbial diversity and few acetoclastic methanogens at higher<br />

flow rates, and conversely, good granulation, good microbial diversity, and many methanogens,<br />

including acetoclastic morphotypes at the lower flow rate. It was concluded that at the higher flow<br />

rate, the washout rate <strong>of</strong> micro-organisms was <strong>of</strong> similar magnitude to their generation rate, and thus<br />

diverse and stable microbial communities failed to establish (Section 6.1.2). It was concluded that<br />

liquid upflow velocity was an important factor for ensuring microbial stability in an ABR <strong>treating</strong><br />

sewage, sludge accumulation rates, and thus ultimately the required desludging interval.<br />

It was further concluded that, since microbial respiration rates were limited by low pH values and low<br />

substrate concentrations, the critical upflow velocity is not a global value, but system specific and<br />

dependent on prevailing pH conditions determined by alkalinity concentration and organic strength <strong>of</strong><br />

the wastewater to be treated.<br />

7.1.1.5 Sludge production rates and condition <strong>of</strong> accumulated solids<br />

It was estimated that sludge accumulated at a rate <strong>of</strong> 0.43 kg dry solids/ kg applied COD at an A-HRT<br />

<strong>of</strong> 22 h (Phase III) and at 0.11 kg dry solids/ kg applied COD at an A-HRT <strong>of</strong> 42 h (Phase IV). These<br />

values corresponded to desludging intervals <strong>of</strong> 105 d and 405 d <strong>of</strong> uninterrupted operation respectively<br />

(Section 6.2.1).<br />

It was concluded that at the higher flow rate, accumulated solids contained significant amounts <strong>of</strong><br />

undegraded particulate organic material since this was not recovered in the effluent. It was concluded<br />

that the main function <strong>of</strong> ABR was a solids accumulator at high flow rates. However, at low flow<br />

rates, approximately 80% <strong>of</strong> incoming COD was removed, while it was calculated that approximately<br />

60% <strong>of</strong> inflow COD was converted to CH4. Therefore, the ABR behaved as a solids digester at these<br />

flow rates, and it is expected that the residual biodegradability <strong>of</strong> the accumulated sludge was far<br />

lower at the lower flow rate than at the higher flow rate. This proposal was supported by the nitrogen<br />

balance, which indicated that little <strong>of</strong> the influent TKN was retained as sludge (Section 6.2.2). The<br />

residual biodegradability <strong>of</strong> the accumulated sludge will have an impact on how removed sludge may<br />

be disposed <strong>of</strong>.<br />

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