novel approaches to expression and detection of oestrus in dairy cows

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Figure 5.1 Figure 5.2 Figure 5.3 Figure 5.4 Figure 5.5 A diagram to show the experimental design of proof of concept trials per day 106 Graph showing mounting between 2 cows with 8 numbered points showing the sequence in time as cow 533 (blue line) stood to be mounted by cow 543 (red line) 115 Graph complementing Figure 5.2 showing only the height changes in sequence of mounting 115 Graph showing mounting between 2 cows over 14 numbered time points. This demonstrates the sequence in time as cow 533 (blue line) stood to be mounted by cow 543 (red line) 116 Graph complementing Figure 5.4, showing only the height changes in the sequence of mounting 116 Figure 5.6 Progesterone profiles from POC 1 of 12 cows 123 Figure 5.7 Progesterone profiles from POC 2 of 8 cows 126 Figure 5.8 Progesterone profiles from POC 3 of 8 cows 128 Figure 5.9 Activity graphs from POC 1, activity units plotted against date and time in 2 hourly intervals for 12 cows 130 Figure 5.10 Activity graphs from POC 2, activity units plotted against date and time in 2 hourly intervals for 8 cows 133 Figure 5.11 Activity graphs from POC 3, activity units plotted against date and time in 2 hourly intervals for 8 cows 135 xii
LIST OF TABLES Table 1.1 Trends in the UK dairy industry 1 Table 1.2 Table 2.1 Table 2.2 Table of behavioural scores relating to particular oestrous behaviour 36 Table of variables investigated for their effect on the increase in activity at oestrus 54 Effect of lactation number, parity, oestrous number and oestrous season on activity at oestrus 58 Table 2.3 Effect of milk yield on activity at oestrus 58 Table 2.4 Table 3.1 The effects of the interaction between milk yield and parity on the activity increase at oestrus 59 SNPs on genes investigated for their associations with an increase in activity at oestrus 70 Table 3.2 PCR reaction reagents 74 Table 4.1 Table 4.2 Table 5.1 Table 5.2 Table 5.3 Table 5.4 Problems and solutions concerning equipment set up for monitoring of dairy cows using UWB 98 Table showing results of 12 hour trial UWB monitoring cows in oestrus 100 Results from POC 2 showing each cow’s mounting behaviour identified from the UWB data, expressed as a percentage of the total mounts by visual observation 117 Results from POC 3 showing each cow’s mounting behaviour identified from the UWB data, expressed as a percentage of the total mounts by visual observation 117 Efficiency and accuracy of the optimised script at identifying mounts from POC 2 on day of oestrus 119 Efficiency and accuracy of the optimised script at identifying mounts from POC 3 on days of oestrus 119 Table 5.5 Number of mounts declared by the script per day in POC 2 121 xiii
Page 1 and 2: NOVEL APPROACHES TO EXPRESSION AND
Page 3 and 4: TABLE OF CONTENTS TABLE OF CONTENTS
Page 5 and 6: 2.2.3 Statistical Analyses ........
Page 7 and 8: 5.3 RESULTS .......................
Page 9 and 10: Furthermore, automated software was
Page 11: LIST OF FIGURES Figure 1.1 Hormonal
Page 15 and 16: LIST OF ABBREVIATIONS ˚ ˚C μM 2D
Page 17 and 18: CHAPTER 1 - Introduction & Literatu
Page 19 and 20: the past 50 years and duration of o
Page 21 and 22: Oestrus growing follicle (Staigmill
Page 23 and 24: ecomes the main inhibitor of FSH an
Page 25 and 26: calf at 40-50 days post partum; inv
Page 27 and 28: al., 2006). However, aged sperm hav
Page 29 and 30: can occur within 2-3 days, but if t
Page 31 and 32: educes the incidence of problem cow
Page 33 and 34: oestradiol, the LH surge and ovulat
Page 35 and 36: The secondary signs of oestrus can
Page 37 and 38: There are also changes in normal be
Page 39 and 40: engage in more natural behaviours i
Page 41 and 42: 1983). Exact explanations and mecha
Page 43 and 44: 2006) and disruption of LH secretio
Page 45 and 46: 1.4.3.2 Milk Yield and Nutrition Di
Page 47 and 48: influence the ability of the ovary
Page 49 and 50: cyclicity can be delayed if dietary
Page 51 and 52: indication for the optimal time to
Page 53 and 54: calving), 3) pre-breeding heat date
Page 55 and 56: 1.5.2 Physiological Changes Physiol
Page 57 and 58: 1.5.2.3 Body and Milk Temperature T
Page 59 and 60: physical activity and stage of the
Page 61 and 62: caused by the general environment t
Page 63 and 64:
may be gained. This is because data
Page 65 and 66:
In summary the objective was to for
Page 67 and 68:
diet, with concentrates at milking.
Page 69 and 70:
oestrus was defined as 3 consecutiv
Page 71 and 72:
The interaction between parity and
Page 73 and 74:
individual oestrus was not signific
Page 75 and 76:
Table 2.4 The effects of the intera
Page 77 and 78:
(361 vs. 578 points, respectively,
Page 79 and 80:
oestrous expression with increasing
Page 81 and 82:
the blood (Sangsritavong et al., 20
Page 83 and 84:
CHAPTER 3 - Single Nucleotide Polym
Page 85 and 86:
population owing to previous select
Page 87 and 88:
Table 3.1 Cont. Follicle Stimulatin
Page 89 and 90:
3.2.4 Sequencing of DNA in the Labo
Page 91 and 92:
was achieved. PCR products were rem
Page 93 and 94:
3.4 DISCUSSION The objectives of th
Page 95 and 96:
fertility and oestrous expression.
Page 97 and 98:
CHAPTER 4 - Development of a Novel
Page 99 and 100:
In summary UWB seems a good option
Page 101 and 102:
Initial tests were carried out to i
Page 103 and 104:
Therefore this demonstrates that X
Page 105 and 106:
which is most important for achievi
Page 107 and 108:
Figure 4.9 Horizontal - Vertical Di
Page 109 and 110:
that UWB is matching the ‘truth
Page 111 and 112:
mounting cow. For example height ch
Page 113 and 114:
Backpack 1 st put on in AI stalls E
Page 115 and 116:
Cows’ behaviour was assessed at 5
Page 117 and 118:
or that the cows were in an area of
Page 119 and 120:
plane, but mostly in achieving high
Page 121 and 122:
Develop techniques for analysis of
Page 123 and 124:
in elastic silicone moulded over a
Page 125 and 126:
and time of mount, duration of moun
Page 127 and 128:
observed matched with increases in
Page 129 and 130:
5.3 RESULTS Results demonstrate pos
Page 131 and 132:
Figure 5.2 Graph showing mounting b
Page 133 and 134:
Table 5.1 Results from POC 2 showin
Page 135 and 136:
Table 5.3 Efficiency and accuracy o
Page 137 and 138:
oestrus and oestrus it is clear to
Page 139 and 140:
P4 Concentration, ng/ml P4 Concentr
Page 141 and 142:
Page 143 and 144:
Page 145 and 146:
Page 147 and 148:
Activity Activity Activity Activity
Page 149 and 150:
Page 151 and 152:
Page 153 and 154:
5.4 DISCUSSION The aim of this work
Page 155 and 156:
averaged 70% which was lower than t
Page 157 and 158:
when the cows lie down, especially
Page 159 and 160:
the level of oestrous activity was
Page 161 and 162:
technology larger herds must be mon
Page 163 and 164:
monitoring could also monitor non-l
Page 165 and 166:
antennae which could affect accurac
Page 167 and 168:
predispose cows to illness e.g. met
Page 169 and 170:
CHAPTER 6 - Overall Discussion & Co
Page 171 and 172:
oestrus, h 2 =0.27 (Lovendahl et al
Page 173 and 174:
The final section of this thesis de
Page 175 and 176:
ate. By using UWB in a commercial s
Page 177 and 178:
provides an insight the potential o
Page 179 and 180:
BLOWEY, R. (2005) Factors associate
Page 181 and 182:
ovine gonadotrophin releasing hormo
Page 183 and 184:
GINTHER, O. J., KOT, K., KULICK, L.
Page 185 and 186:
associated with embryonic survival
Page 187 and 188:
ody condition at parturition on end
Page 189 and 190:
Signs in a 24-h Tie-Stalled Dairy H
Page 191 and 192:
SHERMAN, E. L., NKRUMAH, J. D., MUR
Page 193 and 194:
Influence of negative energy balanc
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