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novel approaches to expression and detection of oestrus in dairy cows

novel approaches to expression and detection of oestrus in dairy cows

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Table 2.1 Def<strong>in</strong>itions <strong>of</strong> cow fac<strong>to</strong>rs analysed for their effects on the activity<br />

<strong>in</strong>crease at <strong>oestrus</strong><br />

Variable Def<strong>in</strong>ition<br />

Days <strong>in</strong> Milk Number <strong>of</strong> days post partum until each <strong>in</strong>dividual <strong>oestrus</strong> event<br />

(DIM)<br />

were <strong>in</strong>vestigated for the association between DIM <strong>and</strong> activity at<br />

<strong>oestrus</strong>.<br />

Milk Yield at The average milk yield was calculated over each <strong>oestrus</strong> period<br />

Oestrus<br />

<strong>to</strong> <strong>in</strong>vestigate the effect <strong>of</strong> level <strong>of</strong> production at the time <strong>of</strong><br />

<strong>oestrus</strong> on the activity <strong>in</strong>crease. Milk yield at <strong>oestrus</strong> was<br />

calculated as the average <strong>of</strong> 5 days before, the day <strong>of</strong> <strong>oestrus</strong><br />

<strong>and</strong> 5 days post <strong>oestrus</strong>. This accounted for daily variation <strong>and</strong><br />

decrease <strong>in</strong> yield that is reported at <strong>oestrus</strong>.<br />

Average Daily The <strong>to</strong>tal milk yield <strong>to</strong> each <strong>oestrus</strong> event was divided by the<br />

Milk Yield number <strong>of</strong> DIM <strong>to</strong> calculate a representative daily average. This<br />

variable was used <strong>to</strong> assess whether the overall level <strong>of</strong> daily<br />

production with<strong>in</strong> each lactation had an effect on oestrous<br />

<strong>expression</strong>.<br />

Cumulative The <strong>to</strong>tal milk yield produced per lactation up <strong>to</strong> each <strong>in</strong>dividual<br />

Lactation Yield <strong>oestrus</strong> event was used <strong>to</strong> assess the effect <strong>of</strong> overall level <strong>of</strong><br />

production on activity.<br />

Parity<br />

Cows were grouped <strong>in</strong><strong>to</strong> parity 1, 2 <strong>and</strong> 3 correspond<strong>in</strong>g <strong>to</strong> <strong>cows</strong><br />

<strong>in</strong> their 1 st parity, 2 nd parity <strong>and</strong> <strong>cows</strong> <strong>in</strong> their 3 rd parity <strong>and</strong><br />

above. These were analysed for the effect <strong>of</strong> age <strong>and</strong> lactation<br />

number on oestrous <strong>expression</strong>.<br />

Oestrous Successive <strong>oestrus</strong>es per lactation were analysed for the effect <strong>of</strong><br />

Number<br />

multiple oestrous cycles on the <strong>expression</strong> <strong>of</strong> <strong>oestrus</strong>. The whole<br />

data set was analysed with <strong>oestrus</strong> events per lactation <strong>in</strong>cluded<br />

<strong>in</strong> statistical analysis as variates, n=930. However this analysis<br />

may <strong>in</strong>clude poor <strong>cows</strong> or <strong>cows</strong> that were later culled for<br />

<strong>in</strong>fertility. The average number <strong>of</strong> oestrous cycles per lactation <strong>in</strong><br />

the <strong>to</strong>tal data set was 3.12, therefore <strong>cows</strong> that conceived at<br />

their 3 rd oestrous cycle were analysed separately for the effect <strong>of</strong><br />

oestrous number on <strong>expression</strong>. Further <strong>in</strong>vestigation then<br />

looked at the difference between the 1 st <strong>and</strong> 2 nd <strong>oestrus</strong> event.<br />

Time <strong>of</strong> Year This variable takes <strong>in</strong><strong>to</strong> account the time <strong>of</strong> year <strong>in</strong> which each<br />

<strong>oestrus</strong> event occurred; 1 - Jan-Mar, 2 – Apr-June, 3 – July-Sept<br />

<strong>and</strong> 4 – Oct-Dec. This was <strong>to</strong> account for environmental variables<br />

such as day length which could physiologically affect the<br />

<strong>expression</strong> <strong>of</strong> <strong>oestrus</strong>.<br />

Successful Activity <strong>in</strong>crease at <strong>oestrus</strong> was analysed for its effect on whether<br />

Conception successful conception was related <strong>to</strong> oestrous <strong>expression</strong>.<br />

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