Pesticide residues in food — 2006: Toxicological ... - ipcs inchem

263
Table 28. Results of studies of genotoxicity with cyromazine
End-point Test object Concentration o Purity (%) Results GLP or
QA
In vitro
Reverse mutation S. typhimurium TA98, TA100, TA1535,
TA1537
Reverse mutation S. typhimurium TA1538 & E. coli
WP2uvrA
Gene mutation, mitotic
gene conversion & mitotic
recombination
20–5000 μg/plate, in DMSO 97.5 Negative a,b GLP &
QA
20–5000 μg/plate, in DMSO 97.5 Negative a,c GLP &
QA
Saccharomyces cerevisiae D7 375–3000 μg/ml, in DMSO 98.9 Negative a,d GLP &
QA
Gene mutation Mouse lymphoma cells, L5178Y, Tk +/- 50–500 μg/ml, in culture medium, 4-h exposure 96.2 Negative a,e GLP &
QA
Gene mutation Chinese hamster, V79 cells, Hprt locus 25–1000 μg/ml, −S9, in ethanol, 21-h exposure
100–4000 μg/ml, +S9, in ethanol, 5-h exposure
Chromosomal aberration Human peripheral blood lymphocytes 62.5, 125, 250, 500 and 1000 μg/ml in DMSO, ± S9,
3-h treatment, harvesting 46 h after the end of treatment
98.9 Negative a,f GLP &
QA
96.2 Negative a,g GLP &
QA
Reference
Deparade
(1988)
Deparade
(1990)
Hool (1984)
Beilstein
(1985)
Dollenmeier
(1986)
Unscheduled DNA synthesis Rat (F344) primary hepatocytes 1–10 -4 mg/ml in DMSO, 18-h exposure NR Negative h QA Tong (1982)
Unscheduled DNA synthesis Mouse (male CD-1) primary
1–10 -4 mg/ml in DMSO, 18-h exposure NR Negative i QA Tong (1983)
hepatocytes
In vivo
98.9 Negative k — Hool (1980)
Nucleus anomalies j (BM cells) Chinese hamster (three male + three
female/group)
Micronucleus test (BM cells) Mouse (Tif:MAGf SPF, NMRI derived)
(five male + five female/group)
Spot test Mouse (males: T-stock, females:
C57Bl/6)
Two oral doses at 2000, 4000 and 8000 mg/kg bw (24 h
apart) in CMC
Single oral doses of 360 and 1080 mg/kg bw in CMC-
Sampling time: 24, 48 and 72 h after treatment
Single intraperitoneal. doses at 150, 300 and 600 mg/kg
bw in sesame oil
96.3 Negative l GLP &
QA
96.2 Inconclusive m GLP &
QA
Dominant lethal Mouse (Tif:MAGf SPF, NMRI derived) Single oral doses at 226 or 678 mg/kg bw in PEG 400 98.9 Negative n — Hool (1981)
CMC, carboxymethyl cellulose; DMSO, dimethyl sulfoxide; GLP, good laboratory practice; NR, not reported; QA, quality assurance; S9, S9, 9000 × g supernatant from livers of male rats.
a
With and without metabolic activation.
b
Cyromazine was 264assayed twice using the standard plate incorporation protocol over a dose range of 20-5000 μg/plate, ±S9 prepared from Aroclor-induced male RAI rats. The
experimental protocol essentially complied with OECD TG 471 (1997).
Strasser
(1985)
Strasser
(1987)
Strasser
(1986)
CYROMAZINE X-X JMPR 2006