Pesticide residues in food — 2006: Toxicological ... - ipcs inchem

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Table 12. Haematological abnormalities in rats fed diets containing bifenazate for 28 days
Parameter
Dietary concentration (ppm)
Males
Females
0 500 1000 5000 0 500 1000 5000
Erythrocyte count (10 9 /ml) 7.66 7.44 7.05* 6.35* 7.71 6.93* 6.34* 5.99*
Haemoglobin (g/dl) 15.4 14.4 14.6* 13.8* 16.0 14.3* 13.6* 13.2*
Erythrocyte volume fraction 0.420 0.417 0.408 0.386* 0.430 0.396* 0.372* 0.368*
From Trutter (1997c)
* p < 0.05
On clinical chemistry examination, BUN concentrations were increased in females at 5000 ppm.
AST activities were increased in females at 5000 ppm and the male at 10 000 ppm. Alkaline phosphatase
activities were decreased in all treated groups, which may be related to decreased bone alkaline
phosphatase activities associated with decreased growth and lower body-weight gain. These findings
are presented in Table 13.
Table 13. Clinical chemistry abnormalities in rats fed diets containing bifenazate for 28 days
Parameter
Dietary concentration (ppm)
Males
Females
0 500 1000 5000 10 000 a 0 500 1000 5000
Blood urea nitrogen (mg/dl) 14 13 13 17 21 13 15 15 32*
Aspartate aminotrasferase (U/l) 75 79 69 90 136 71 71 66 109*
Alkaline phosphatase (U/l) 169 142* 133* 104* 106 104 77* 83 62*
From Trutter (1997c)
* p < 0.05
a
There was only one survivor in this group.
There were no abnormalities detected on urine analysis. On gross examination post mortem
of rats dying during the study, dark livers and dark areas in the stomach were seen in males at
10 000 ppm and females at 5000 and 10 000 ppm. At terminal sacrifice, no gross abnormalities were
seen. Increased adrenal and liver weights relative to body weight were seen at 1000 ppm and above.
Absolute and relative adrenal and liver weights are presented in Table 14.
Table 14. Organ-weight changes in rats fed diets containing bifenazate for 28 days
Organ weight changes
Dietary concentration (ppm)
Males
Females
0 500 1000 5000 0 500 1000 5000
Adrenal weight (g) 0.062 0.055 0.064 0.064 0.077 0.066* 0.073 0.059*
Adrenal weight relative to body
weight (%)
0.015 0.014 0.020* 0.025* 0.032 0.030 0.038* 0.043*
Liver weight (g) 12.01 12.61 10.62 11.19 7.48 7.16 8.06 7.05
Liver weight relative to body 2.91 3.13 3.24* 4.57* 3.13 3.24 4.15* 5.15*
weight (%)
From Trutter (1997c)
* p < 0.05
BIFENAZATE X-X JMPR 2006