Vol 21 No. 1

J Bagh College Dentistry Vol. 21(1), 2009 Distribution and localization of…
K
S
S
BC
BM
Figure 5: Inflammatory gingival
enlargement showing positive PAS reaction
in keratinized epithelia (K), in granular
layer (G) and in some layer of spinosum(S).
Basement membrane (BM) shows positive
stain too. PAS stain X 10
Figure 6: High magnifying view of inflammatory
gingival enlargement showing negative PAS
reaction in basal cell (BC) and Spinosum Cell (SC)
while basement membrane and Lamina propria
show positive reaction (arrow) PAS stain X 20
BM
LP
Figure 7: Inflammatory gingiva showing positive PAS stain in Basement membrane (BM) and in
Lamina propria (LP). PAS stain X 20
Specimens from gingival of Phenytoin
induced drug showed localization of positive
PAS reaction in spinosum layer while negative
stain showed in basement membrane and in
connective tissue. (Figures 2-4).
Histopathological slides for specimens of
inflammatory gingival stained with PAS showed
deep reddish purple stain in keratinized layer,
granulosum layer and includes some layers of
spinosum, basement membrane shows positive
stain (figures 5,6). Figure 7 showed band of
reddish purple stain in connective tissue
underneath the epithelium.
DISCUSSION
The gingival tissue consist of epithelial cells,
collagenous fibers, connective tissue cells,
intercellular substance (ground substance) which
includes glycogen, glycoprotein, proteoglycan
and mucin. Capillaries, arterioles and venules as
well as lymph vessels and nerves are also
present.
The macromolecular carbohydrate
components in epithelium and connective tissue
of gingival are secreted by epithelial cell and
(13)
fibroblast cells (respectively). The
carbohydrate group includes glycogen,
glycoprotein and proteoglycans detected by
periodic acid Schiff (PAS) method.
Changes in epithelial glycogen showed
during inflammation and repair and even
variation in keratinization may reflect the
glycogen content of tissue (4) . Thus the present
results showed an intense reaction of PAS stain
in area of keratin and granular layer of
inflammatory gingival which related to an
increase of production of glycogen by epithelial
cells basement membrane exhibit high PAS
reaction which may relate to increase in
fibronectin and lamanin (glycoprotein). As
basement membrane separates between the
epithelium and connective tissue, fibronectin
secretes by fibroblast in connective tissue while
lamanin secreted by epithelium cells.
Both proteoglycans and glycoprotein in
connective tissue undergo alteration in various
pathological state, therefore during inflammation
there is an increase in both glycoprotein and
proteoglycan level correlated with pathological
Oral Diagnosis 51