Vol 21 No. 1

J Bagh College Dentistry Vol. 21(1), 2009 The role of lipid peroxidation …
Saliva: Three mls of unstimulated (resting)
whole saliva from each subjects was collected
over ice and samples were centrifuged and frozen
at -20 o C until analysis.
Determination of plasma and saliva MDA:
Lipid peroxidation end products, particularly
malondialdehyde (MDA) react with thiobarbituric
acid under acidic conditions and heating to give a
pink color that measured spectrophotometricaly at
532 nm. The procedure is according to Shah and
Walker. (10)
Determination of plasma and saliva thiol:
5.5'- dithios (2-nitrobenzoic acid) (DTNB) is
a disulfide chromogen that is readily reduced by
sulfhydryl compounds to an intensely yellow
compound. The absorbance of the reduced
chromogen is measured spectrophotometricaly at
420 nm. and is directly proportional to the (SH)
concentration. The procedure is according to
Ellman.
Estimation of erythrocyte glutathione
erythrocyte (GSH):
The method for determining GSH is based on
the development of a yellow color when 5.5'
dithiobis (2 nitro benzoic acid) (DTNB) is added
to sulphydryl compound. The reaction is
measured spectrophotometrically at 412 nm
according to Beutler etal (9)
Statistical analysis:
Statistical package for the social sciences
(SPSS) was used; mean and standard deviation
(S.D.) of each parameters were calculated. The
independent sample T-test program was used to
get the significance level (p-value) for all the
parameters tested. A p-value less than 0.05 are
considered significant, less than 0.001 is highly
significant, and more than 0.05 is non-significant.
RESULTS
1. Age and gender.
Fifty patients with chronic periodontitis, their
age range was (20-60) years with mean age of
32.6 ± 10.3, they were 30 females and 20 males,
and fifty healthy control subjects of age range
(22-60) years with mean age 34 ± 10.5, they were
27 females and 23 males.
2. Plasma and saliva MDA.
Plasma and saliva MDA was found to be
significantly higher p