Biosafety Guidelines For Research On Genetically Modified ...

APPENDIX 6: PROCEDURES FOR WORK WITH HAZARDOUS FRAGMENTS OF
DNA
Note for Category B (iv), Section 4.2
When working with isolated DNA molecules or amplifying DNA molecules using
techniques such as the polymerase chain reaction (PCR), there are some cases where
caution is warranted. Some degree of risk may exist and the extent of this is uncertain.
Such cases include:
1. DNA which encodes an active oncogene product or tumour suppressor gene
product, particularly when this is associated with a gene promoter with high
activity in human cells. DNA containing more than one active oncogene is
associated with increased risk.
2. DNA encoding growth factors, their receptors or other substances that might
directly or indirectly alter the growth patterns of human cells.
3. DNA or RNA representing complete viral genomes or fragments with the
potential to regenerate live virus. Complete genomes for HIV or papilloma
viruses, for example, warrant careful handling.
There is some risk that such molecules could enter the cells of the operator, the
principal routes of entry being through breaks in the skin. It is therefore recommended
that work of this type be carried out using gloves in order to avoid skin contact. Special
care shall be taken when using needles or other sharp instruments.
Precautions for handling genetically manipulated viruses with the capacity to infect
human cells are described in Appendix 5.
Appendix - Page 15