Biosafety Guidelines For Research On Genetically Modified ...

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Points to consider This section lists some points to consider in the design of facilities for genetic manipulation work involving the production of transgenic animals. The containment required for transgenic animals varies among different experimental systems. The following points govern the nature of containment involved: Details of Animal Involved 1. Class, genus and species of animal (if transgenic aquatic vertebrates are involved see Appendix 18) 2. Stages in life cycle that will be involved (embryo only to full maturity and reproduction). 3. Approximate number of animals involved at any one time. Type of Work 4. Whether the work will involve: (a) modification of germline cells; (b) modification of somatic cells; (c) intention to breed; (d) use of genetically modified micro-organisms to infect animals; (e) infectious agents used to produce transgenic animals Details of the Genetic Manipulation 5. Depending on the type of work, the following points should be considered before deciding upon the level of animal containment (GA-BSL2 & GA-BSL3): (i) nature of DNA to be inserted (origin and characterisation of DNA, references to published work, reference numbers of small-scale proposals covering preparation of the DNA); (ii) method to be used for introducing the DNA (including details of the construction of any vector to be used, references to published work or reference numbers of small-scale proposals covering preparation of the vector, and containment level recommended for work with the vector); (iii) likelihood of the technique to be used for introducing the DNA resulting in the animal bearing an agent potentially transmissible to animals in the same facility or to humans, and the possible routes of transmission; (iv) reproductive capability of the genetically manipulated animal - is the inserted DNA likely to be heritable Appendix - Page 27
Other Work Being Performed in the Same Holding Facility 6. Are there any other animals being housed in the same facility If so, (i) how many animals are being housed (ii) are they all the same species and strain (what other species are involved) (iii) what is the nature of any experimental work being carried out on the other animals (for example, infectious-disease work, or genetic manipulation work) Is it likely that these experiments will interfere with the proposed work (iv) what arrangements are there to ensure that the other animals are kept separate from the transgenic animals (v) what procedures are in place for the identification of, and accounting for, individual animals Disposal of Animals 7. Bearing in mind the points to consider and the information provided above, consider what special precautions are needed, in addition to standard animal-house practices, regarding the disposal of animals. Transport of Animals 8. If it is necessary to transport the animals alive from the holding facility to the laboratory or another facility, consider what precautions are needed to be taken in addition to the provisions set out in Section 6.2 of the <strong>Guidelines</strong>. Appendix - Page 28
Page 1 and 2:
THE SINGAPORE BIOSAFETY GUIDELINES
Page 3 and 4:
APPENDICES Appendix 1 Other Relevan
Page 5 and 6:
1.4 DEFINITIONS OF ABBREVIATIONS Fo
Page 7 and 8:
2.2 EXEMPTIONS 2.2.1 The following
Page 9 and 10:
SECTION 3: SUMMARY OF PROCEDURES 3.
Page 11 and 12:
3.3 CATEGORY A - EXPERIMENTS REQUIR
Page 13 and 14: SECTION 4: EXPERIMENTS COVERED BY T
Page 15 and 16: 4.2 CATEGORY B - EXPERIMENTS REQUIR
Page 17 and 18: SECTION 5: ROLES AND RESPONSIBILITI
Page 19 and 20: 5.2.5 Monitoring of Work The IBC sh
Page 21 and 22: • submit a new proposal form to t
Page 23 and 24: • All materials that are genetica
Page 25 and 26: 6.5 SUPPLY OF GENETICALLY MANIPULAT
Page 27 and 28: 6.6.5 The form should contain any r
Page 29 and 30: BLANK PAGE
Page 31 and 32: 3. Worldwide guidelines specific to
Page 33 and 34: xiv. AS 2252.2: 1994: xv. AS 2647:
Page 35 and 36: APPENDIX 2: LIST OF GMAC-APPROVED H
Page 37 and 38: APPENDIX 3: INSTRUCTIONS FOR COMPLE
Page 39 and 40: PROPOSAL FORM FOR ASSESSMENT OF GEN
Page 41 and 42: -----------------------------------
Page 43 and 44: ) Describe the techniques to be emp
Page 45 and 46: ii) Category B iii) Category C C. R
Page 47 and 48: APPENDIX 4: TOXINS For work involvi
Page 49 and 50: 4. Procedures for handling viruses
Page 51 and 52: staff shall handle these infected a
Page 53 and 54: APPENDIX 7: REQUIREMENTS FOR GENETI
Page 55 and 56: equire the provision of a front cov
Page 57 and 58: Signs 10. An automatically-starting
Page 59 and 60: 9. All plants in the plant house sh
Page 61 and 62: APPENDIX 12: PROCEDURES FOR EXPERIM
Page 63: APPENDIX 13: DESIGN OF ANIMAL FACIL
Page 67 and 68: permitted in animal rooms. Personne
Page 69 and 70: APPENDIX 15: REQUIREMENTS FOR GENET
Page 71 and 72: APPENDIX 16: REQUIREMENTS FOR INSEC
Page 73 and 74: APPENDIX 17: REQUIREMENTS FOR INSEC
Page 75 and 76: APPENDIX 18: PROCEDURES FOR EXPERIM
Page 77 and 78: From Bio-Security Assurance Arrange
Page 79 and 80: VIRUSES (con’t) Family and genus
Page 81 and 82: PESTS (cont’d) INSECTS MAIN HOST
Page 83 and 84: PESTS (cont’d) NEMATODES MAIN HOS
Page 85 and 86: PESTS (cont’d) NEMATODES MAIN HOS
Page 87 and 88: From the Notifiable Diseases List o
Page 89 and 90: From the Notifiable Diseases List o
Page 91 and 92: APPENDIX 20: GLOSSARY Note: Words a
Page 93 and 94: fusion gamete gene gene therapy gen
Page 95 and 96: polymerase chain reaction prion pro
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