Biosafety Guidelines For Research On Genetically Modified ...

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containment containment level defective virus deliberate release DNA donor Drosophila embryo-rescue Escherichia coli (E. coli) Escherichia coli K12 Escherichia coli B eukaryotic expression Prevention of the spread of genetically manipulated organisms outside the laboratory. Physical containment is accomplished by the use of special procedures and facilities. Biological containment is accomplished by the use of particular strains of the organism which have a reduced ability to survive or reproduce in the open environment. The degree of physical containment provided by a laboratory or facility, which depends on the design of the facility, the equipment installed, and the procedures used. GMAC physical containment levels are numbered from 2 to 4, 4 being the highest level. A virus that is unable to reproduce in its host without the presence of another (‘helper’) virus. Intentional release of a genetically modified organism into the open environment. Deoxyribonucleic acid, the molecule which carries the genetic information for most organisms; consists of four bases and a sugar-phosphate backbone. The organism or cell from which DNA is derived for insertion into another organism (the host). A genus of flies whose genetics has been extensively studied. The process in plant breeding whereby tissue from young embryo plants is excised and propagated in vitro for subsequent growth as differentiated plants. A bacterium that inhabits the intestinal tract of humans (and other animals). A strain of E. coli that has been maintained in culture in laboratories for many years. It has lost the ability to colonise the intestinal tract of humans and animals, is well-characterised genetically, and is often used for molecular cloning work. Another well-characterised laboratory strain of E. coli. Belonging to the group of organisms whose cells contain a true nucleus. Eukaryotic organisms include animals, plants and fungi. Manifestation of a characteristic that is specified by a gene; often used to mean the production of a protein by a gene that has been inserted into a host organism. fungi Non-photosynthetic eukaryotic organisms, including moulds, that feed on organic matter. Appendix - Page 55
fusion gamete gene gene therapy genetic engineering genetic manipulation genome genotype germline cells GA-BSL GI-BSL GM-BSL GP-BSL GMAC GMO growth factor helper virus HEPA filter HIV host Joining of the cell membranes of two cells to create a daughter cell that contains the genetic material from both parent cells. A reproductive (egg or sperm) cell. A hereditary unit of nucleic acid which specifies the structure of a protein or RNA molecule. The replacement of a defective gene in a person or other animal suffering from a genetic disease. See genetic manipulation. A technology used to alter the genetic material of living cells or organisms in order to make them capable of producing new substances or performing new functions. The total genetic complement of a given organism. The genetic make-up of an organism, as distinguished from its physical appearance (the phenotype). Gametes and the cells from which they are derived. The genetic material of germline cells, unlike that of somatic cells (q.v.), can be passed to succeeding generations. Biosafety Level for genetic modification of animal Biosafety Level for genetic modification of insects Biosafety Level for general genetic modification work Biosafety Level for genetic modification of plants Genetic Modification Advisory Committee. Genetically Modified Organism. A protein that stimulates cell division when it binds to its specific cell-surface receptor. A virus which, when used to infect cells already infected by a defective virus (q.v.), enables the latter to multiply by supplying something the defective virus lacks. High efficiency particulate air filter. Human immunodeficiency virus (a retrovirus). A cell or organism into which foreign DNA is introduced to enable production of proteins or further quantities of the DNA. Appendix - Page 56
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THE SINGAPORE BIOSAFETY GUIDELINES
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APPENDICES Appendix 1 Other Relevan
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1.4 DEFINITIONS OF ABBREVIATIONS Fo
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2.2 EXEMPTIONS 2.2.1 The following
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SECTION 3: SUMMARY OF PROCEDURES 3.
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3.3 CATEGORY A - EXPERIMENTS REQUIR
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SECTION 4: EXPERIMENTS COVERED BY T
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4.2 CATEGORY B - EXPERIMENTS REQUIR
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SECTION 5: ROLES AND RESPONSIBILITI
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5.2.5 Monitoring of Work The IBC sh
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• submit a new proposal form to t
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• All materials that are genetica
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6.5 SUPPLY OF GENETICALLY MANIPULAT
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6.6.5 The form should contain any r
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BLANK PAGE
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3. Worldwide guidelines specific to
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xiv. AS 2252.2: 1994: xv. AS 2647:
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APPENDIX 2: LIST OF GMAC-APPROVED H
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APPENDIX 3: INSTRUCTIONS FOR COMPLE
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PROPOSAL FORM FOR ASSESSMENT OF GEN
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Page 43 and 44: ) Describe the techniques to be emp
Page 45 and 46: ii) Category B iii) Category C C. R
Page 47 and 48: APPENDIX 4: TOXINS For work involvi
Page 49 and 50: 4. Procedures for handling viruses
Page 51 and 52: staff shall handle these infected a
Page 53 and 54: APPENDIX 7: REQUIREMENTS FOR GENETI
Page 55 and 56: equire the provision of a front cov
Page 57 and 58: Signs 10. An automatically-starting
Page 59 and 60: 9. All plants in the plant house sh
Page 61 and 62: APPENDIX 12: PROCEDURES FOR EXPERIM
Page 63 and 64: APPENDIX 13: DESIGN OF ANIMAL FACIL
Page 65 and 66: Other Work Being Performed in the S
Page 67 and 68: permitted in animal rooms. Personne
Page 69 and 70: APPENDIX 15: REQUIREMENTS FOR GENET
Page 71 and 72: APPENDIX 16: REQUIREMENTS FOR INSEC
Page 73 and 74: APPENDIX 17: REQUIREMENTS FOR INSEC
Page 75 and 76: APPENDIX 18: PROCEDURES FOR EXPERIM
Page 77 and 78: From Bio-Security Assurance Arrange
Page 79 and 80: VIRUSES (con’t) Family and genus
Page 81 and 82: PESTS (cont’d) INSECTS MAIN HOST
Page 83 and 84: PESTS (cont’d) NEMATODES MAIN HOS
Page 85 and 86: PESTS (cont’d) NEMATODES MAIN HOS
Page 87 and 88: From the Notifiable Diseases List o
Page 89 and 90: From the Notifiable Diseases List o
Page 91: APPENDIX 20: GLOSSARY Note: Words a
Page 95 and 96: polymerase chain reaction prion pro
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