Biosafety Guidelines For Research On Genetically Modified ...

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APPENDIX 6: PROCEDURES FOR WORK WITH HAZARDOUS FRAGMENTS OF DNA Note for Category B (iv), Section 4.2 When working with isolated DNA molecules or amplifying DNA molecules using techniques such as the polymerase chain reaction (PCR), there are some cases where caution is warranted. Some degree of risk may exist and the extent of this is uncertain. Such cases include: 1. DNA which encodes an active oncogene product or tumour suppressor gene product, particularly when this is associated with a gene promoter with high activity in human cells. DNA containing more than one active oncogene is associated with increased risk. 2. DNA encoding growth factors, their receptors or other substances that might directly or indirectly alter the growth patterns of human cells. 3. DNA or RNA representing complete viral genomes or fragments with the potential to regenerate live virus. Complete genomes for HIV or papilloma viruses, for example, warrant careful handling. There is some risk that such molecules could enter the cells of the operator, the principal routes of entry being through breaks in the skin. It is therefore recommended that work of this type be carried out using gloves in order to avoid skin contact. Special care shall be taken when using needles or other sharp instruments. Precautions for handling genetically manipulated viruses with the capacity to infect human cells are described in Appendix 5. Appendix - Page 15
APPENDIX 7: REQUIREMENTS FOR GENETIC MODIFICATION BIOASFETY LEVEL 2 (GM-BSL2) The requirements listed here for GM-BSL2 containment emphasise the procedures to be observed by the laboratory worker to ensure a basic level of laboratory safety. There are also some architectural requirements relating to laboratory design and equipment. The requirements for Biosafety Level 2 in the Laboratory Biosafety Manual (3 rd Ed, WHO) and the OSH Guidelines for Laboratories and Production Facilities in the Biomedical Sciences Industry shall apply for GM-BSL2. In addition, the following requirements must be included. Laboratory Procedures Many of the laboratory procedures detailed below are those that would be considered essential minimum practices in any microbiological laboratory. 1. Storage of food or drink in the laboratory or any storage unit containing genetically manipulated DNA (e.g. refrigerator) is prohibited. 2. Equipment used for handling cultures or contaminated material which is not readily steam sterilised shall be chemically disinfected after use. 3. All technical procedures shall be performed in a way that minimises the creation of aerosols. In particular, operations such as sonication or vortexing which may generate aerosols are to be done in a biological safety cabinet. A period of at least five minutes shall be allowed for aerosols to settle before opening homogeniser or sonicator containers in a biological safety cabinet. Essential Equipment Each laboratory shall be equipped with basin mixers for hand washing, preferably footoperated, elbow-operated or electronically operated. Emergency drench showers and eyewash stations shall be provided. Laboratory Signs The laboratory shall be labelled with adhesive signs: • on the door, with a sign designating the level of containment; • in the laboratory, with a sign giving procedures required for work at this level of containment. The signs for laboratories shall be put up only after inspection by the relevant regulatory agency for all higher levels of containment. Constant Temperature Rooms Walk-in warm rooms and cold rooms used for incubation or storage of genetically manipulated material may be certified as GM-BSL2 facilities even if they do not contain sinks or coat hooks. Work in such rooms shall otherwise follow all the procedures required for GM-BSL2 laboratories. Appendix - Page 16
Page 1 and 2: THE SINGAPORE BIOSAFETY GUIDELINES
Page 3 and 4: APPENDICES Appendix 1 Other Relevan
Page 5 and 6: 1.4 DEFINITIONS OF ABBREVIATIONS Fo
Page 7 and 8: 2.2 EXEMPTIONS 2.2.1 The following
Page 9 and 10: SECTION 3: SUMMARY OF PROCEDURES 3.
Page 11 and 12: 3.3 CATEGORY A - EXPERIMENTS REQUIR
Page 13 and 14: SECTION 4: EXPERIMENTS COVERED BY T
Page 15 and 16: 4.2 CATEGORY B - EXPERIMENTS REQUIR
Page 17 and 18: SECTION 5: ROLES AND RESPONSIBILITI
Page 19 and 20: 5.2.5 Monitoring of Work The IBC sh
Page 21 and 22: • submit a new proposal form to t
Page 23 and 24: • All materials that are genetica
Page 25 and 26: 6.5 SUPPLY OF GENETICALLY MANIPULAT
Page 27 and 28: 6.6.5 The form should contain any r
Page 29 and 30: BLANK PAGE
Page 31 and 32: 3. Worldwide guidelines specific to
Page 33 and 34: xiv. AS 2252.2: 1994: xv. AS 2647:
Page 35 and 36: APPENDIX 2: LIST OF GMAC-APPROVED H
Page 37 and 38: APPENDIX 3: INSTRUCTIONS FOR COMPLE
Page 39 and 40: PROPOSAL FORM FOR ASSESSMENT OF GEN
Page 41 and 42: -----------------------------------
Page 43 and 44: ) Describe the techniques to be emp
Page 45 and 46: ii) Category B iii) Category C C. R
Page 47 and 48: APPENDIX 4: TOXINS For work involvi
Page 49 and 50: 4. Procedures for handling viruses
Page 51: staff shall handle these infected a
Page 55 and 56: equire the provision of a front cov
Page 57 and 58: Signs 10. An automatically-starting
Page 59 and 60: 9. All plants in the plant house sh
Page 61 and 62: APPENDIX 12: PROCEDURES FOR EXPERIM
Page 63 and 64: APPENDIX 13: DESIGN OF ANIMAL FACIL
Page 65 and 66: Other Work Being Performed in the S
Page 67 and 68: permitted in animal rooms. Personne
Page 69 and 70: APPENDIX 15: REQUIREMENTS FOR GENET
Page 71 and 72: APPENDIX 16: REQUIREMENTS FOR INSEC
Page 73 and 74: APPENDIX 17: REQUIREMENTS FOR INSEC
Page 75 and 76: APPENDIX 18: PROCEDURES FOR EXPERIM
Page 77 and 78: From Bio-Security Assurance Arrange
Page 79 and 80: VIRUSES (con’t) Family and genus
Page 81 and 82: PESTS (cont’d) INSECTS MAIN HOST
Page 83 and 84: PESTS (cont’d) NEMATODES MAIN HOS
Page 85 and 86: PESTS (cont’d) NEMATODES MAIN HOS
Page 87 and 88: From the Notifiable Diseases List o
Page 89 and 90: From the Notifiable Diseases List o
Page 91 and 92: APPENDIX 20: GLOSSARY Note: Words a
Page 93 and 94: fusion gamete gene gene therapy gen
Page 95 and 96: polymerase chain reaction prion pro

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