Biosafety Guidelines For Research On Genetically Modified ...

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Operating procedures 6. The insectary shall only be accessible to those people directly involved in laboratory work. Works are to be carried out by personnel having specific training in handling insects and are directed by competent scientists. 7. Personnel shall decontaminate their hands by washing with soap and warm water in the wash-basin provided on entering and leaving the insectary. When entering, personnel shall put on overshoes and covering clothes (boiler suit style) and hat in the anteroom. Before personnel leave the insectary, stray insects shall be removed from these garments with the vacuum device provided, and the garments removed and kept in the anteroom between uses. Prior to washing (or disposal), garments shall be sealed in bags and autoclaved. 8. Materials taken into and out of the insectary shall be treated with a technique demonstrated to be effective in destroying or removing all stages of the life-cycle of insects and their pathogens. This requirement applies to soil substitutes and soil. Soil substitutes which can be readily decontaminated shall be used in preference to soil. Appendix - Page 37
APPENDIX 18: PROCEDURES FOR EXPERIMENTS INVOLVING FISH AND OTHER AQUATIC ORGANISMS Genetic manipulation work involving the use of fish and other aquatic organisms includes: • production or use of transgenic aquatic organisms; • use of genetically modified microorganisms to infect aquatic organisms. The primary objective in designing containment facilities and procedures for genetic manipulation work involving aquatic organisms is to prevent escape of transgenic aquatic organisms or recombinant infectious agents into natural waterways. Proposals for such work will be examined by IBC on a case-by-case basis. Investigators submitting proposals for research involving transgenic aquatic organisms shall describe in the proposal the containment facilities to be used and procedures proposed for treatment of waste water from the facility. Adherence to the biosafety guidelines does not exempt researchers from practising responsible laboratory animal care and maintain basic welfare standards (useful references: Guide for the Care and Use of Laboratory Animals, National <strong>Research</strong> Council, 1996 and Guide to the Care and Use of Experimental Animals, Canadian Council on Animal Care). <strong>Research</strong>ers should also be aware that they are subject to Singapore laws that legislate against cruelty to animals. This is contained in Part IV of the Animals and Birds Act (Cap. 7). The following general guidelines are provided regarding the basic requirements for genetic manipulation work involving aquatic organisms. General • The rearing area shall be confined in a secured building and be restricted to authorized personnel only. • All water shall leave the plant through a common drain. • All effluent water shall be passed through at least two screens. The size of the screening shall be set to retain the smallest life history stage of the organisms in use. The screens shall be cleared regularly to prevent blockage and overflow. • Effluent water shall not discharge into a major system containing related fish species. • The building shall be structurally sound and of sufficient elevation to preclude flooding or unintentional escape of these transgenic organisms. Work with transgenic aquatic organisms • To prevent escape of adult aquatic organisms into the sewerage system, a grill or mesh, with an appropriate grid size to prevent passage of adults of the species being used, shall be fitted to outlets used for disposal of tank water. • Water from tanks containing only adult transgenic aquatic organisms, and not involving use of recombinant infectious agents, may be discarded untreated down the sink, provided that a filtering mechanism to retain adult aquatic organisms is in place. • Tank water that has any potential to contain embryos, sperm, eggs or larvae of transgenic aquatic organisms shall be treated to ensure inactivation of viable Appendix - Page 38
Page 1 and 2:
THE SINGAPORE BIOSAFETY GUIDELINES
Page 3 and 4:
APPENDICES Appendix 1 Other Relevan
Page 5 and 6:
1.4 DEFINITIONS OF ABBREVIATIONS Fo
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2.2 EXEMPTIONS 2.2.1 The following
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SECTION 3: SUMMARY OF PROCEDURES 3.
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3.3 CATEGORY A - EXPERIMENTS REQUIR
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SECTION 4: EXPERIMENTS COVERED BY T
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4.2 CATEGORY B - EXPERIMENTS REQUIR
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SECTION 5: ROLES AND RESPONSIBILITI
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5.2.5 Monitoring of Work The IBC sh
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• submit a new proposal form to t
Page 23 and 24: • All materials that are genetica
Page 25 and 26: 6.5 SUPPLY OF GENETICALLY MANIPULAT
Page 27 and 28: 6.6.5 The form should contain any r
Page 29 and 30: BLANK PAGE
Page 31 and 32: 3. Worldwide guidelines specific to
Page 33 and 34: xiv. AS 2252.2: 1994: xv. AS 2647:
Page 35 and 36: APPENDIX 2: LIST OF GMAC-APPROVED H
Page 37 and 38: APPENDIX 3: INSTRUCTIONS FOR COMPLE
Page 39 and 40: PROPOSAL FORM FOR ASSESSMENT OF GEN
Page 41 and 42: -----------------------------------
Page 43 and 44: ) Describe the techniques to be emp
Page 45 and 46: ii) Category B iii) Category C C. R
Page 47 and 48: APPENDIX 4: TOXINS For work involvi
Page 49 and 50: 4. Procedures for handling viruses
Page 51 and 52: staff shall handle these infected a
Page 53 and 54: APPENDIX 7: REQUIREMENTS FOR GENETI
Page 55 and 56: equire the provision of a front cov
Page 57 and 58: Signs 10. An automatically-starting
Page 59 and 60: 9. All plants in the plant house sh
Page 61 and 62: APPENDIX 12: PROCEDURES FOR EXPERIM
Page 63 and 64: APPENDIX 13: DESIGN OF ANIMAL FACIL
Page 65 and 66: Other Work Being Performed in the S
Page 67 and 68: permitted in animal rooms. Personne
Page 69 and 70: APPENDIX 15: REQUIREMENTS FOR GENET
Page 71 and 72: APPENDIX 16: REQUIREMENTS FOR INSEC
Page 73: APPENDIX 17: REQUIREMENTS FOR INSEC
Page 77 and 78: From Bio-Security Assurance Arrange
Page 79 and 80: VIRUSES (con’t) Family and genus
Page 81 and 82: PESTS (cont’d) INSECTS MAIN HOST
Page 83 and 84: PESTS (cont’d) NEMATODES MAIN HOS
Page 85 and 86: PESTS (cont’d) NEMATODES MAIN HOS
Page 87 and 88: From the Notifiable Diseases List o
Page 89 and 90: From the Notifiable Diseases List o
Page 91 and 92: APPENDIX 20: GLOSSARY Note: Words a
Page 93 and 94: fusion gamete gene gene therapy gen
Page 95 and 96: polymerase chain reaction prion pro
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