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Biosafety Guidelines For Research On Genetically Modified ...

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polymerase chain reaction<br />

prion<br />

prokaryotic<br />

promoter<br />

protein<br />

protoplast<br />

receptor<br />

recombinant<br />

recombinant DNA<br />

A technique for generating, in vitro, an increased quantity<br />

of a target segment of DNA.<br />

An infectious agent of unknown etiology which causes<br />

spongiform encephalopathies of humans and animals.<br />

Belonging to the group of microorganisms whose DNA is<br />

not enclosed within a nuclear membrane.<br />

A DNA sequence, located in front of a gene, that controls<br />

expression of the gene. It is the sequence to which RNA<br />

polymerase binds to initiate transcription.<br />

A molecule composed of amino acids.<br />

A plant or bacterial cell which has had the outer cell wall<br />

removed.<br />

Cell-surface protein to which molecules such as hormones<br />

and growth factors bind to exert their effects on the cell, or<br />

to which viruses bind to gain entry to the cell.<br />

Organisms, cells, viruses etc. which contain recombinant<br />

DNA (q.v.).<br />

DNA formed by joining, in vitro, segments of DNA from<br />

different organisms.<br />

recombination The occurrence or production of progeny with<br />

combinations of genes other than those that occurred in<br />

the parents.<br />

replication<br />

retroviral vector<br />

retrovirus<br />

RNA<br />

sharps<br />

shot-gun cloning<br />

Reproduction.<br />

A retrovirus (q.v.) which is used to introduce foreign DNA<br />

into animal cells, usually by replacing part of the viral<br />

genome with the foreign DNA of interest.<br />

A virus that uses the enzyme reverse transcriptase to copy<br />

its RNA genome into DNA, which then integrates into the<br />

host cell genome.<br />

Ribonucleic acid, a molecule similar to DNA, whose<br />

functions include decoding the instructions for protein<br />

synthesis that are carried by the genes; comprises the<br />

genetic material of some viruses.<br />

Sharp laboratory items such as syringe needles, scalpel<br />

and razor blades, and broken glass.<br />

The production of a large random collection of cloned<br />

fragments of the DNA of an organism, from which genes<br />

of interest can later be selected.<br />

Appendix - Page 58

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