Biosafety Guidelines For Research On Genetically Modified ...

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host range host-vector system hybridoma IBC in vitro in vivo knockout mouse LD50 microorganism MOH MOM monoclonal antibody NEA oncogene oocyte packaging pathogen PCR phage plasmid <strong>For</strong> a virus, the range of species that can be infected by that virus. Combination of host (q.v.) and the vector (q.v.) used for introducing foreign DNA into the host. A hybrid cell, used in production of monoclonal antibodies (q.v.), which is produced by fusing an antibody-producing cell (a B lymphocyte) with a tumour cell. Institutional <strong>Biosafety</strong> Committee. Literally ‘in glass’; performed in a test tube or other laboratory apparatus. In a living organism. A mouse that has been genetically modified by deletion or inactivation of a specific gene. The dose of a toxin or infectious agent which will kill half of a population of organisms. An organism that can be seen only with the aid of a microscope. Ministry of Health Ministry of Manpower An antibody that is derived from a single clone ( q.v.) of hybridoma (q.v.) cells and recognises only one antigen. National Environment Agency An activated (modified) cellular gene which causes normal cells to become cancerous. A cell that divides to form the female reproductive cell. In the process of virus replication, the assembly of the components of the virus to form the complete virus particle. An organism that causes disease. See polymerase chain reaction. See bacteriophage. A small, self-replicating molecule of DNA which contains a specific origin of replication. Plasmids are often used as cloning vectors (q.v.). Appendix - Page 57
polymerase chain reaction prion prokaryotic promoter protein protoplast receptor recombinant recombinant DNA A technique for generating, in vitro, an increased quantity of a target segment of DNA. An infectious agent of unknown etiology which causes spongiform encephalopathies of humans and animals. Belonging to the group of microorganisms whose DNA is not enclosed within a nuclear membrane. A DNA sequence, located in front of a gene, that controls expression of the gene. It is the sequence to which RNA polymerase binds to initiate transcription. A molecule composed of amino acids. A plant or bacterial cell which has had the outer cell wall removed. Cell-surface protein to which molecules such as hormones and growth factors bind to exert their effects on the cell, or to which viruses bind to gain entry to the cell. Organisms, cells, viruses etc. which contain recombinant DNA (q.v.). DNA formed by joining, in vitro, segments of DNA from different organisms. recombination The occurrence or production of progeny with combinations of genes other than those that occurred in the parents. replication retroviral vector retrovirus RNA sharps shot-gun cloning Reproduction. A retrovirus (q.v.) which is used to introduce foreign DNA into animal cells, usually by replacing part of the viral genome with the foreign DNA of interest. A virus that uses the enzyme reverse transcriptase to copy its RNA genome into DNA, which then integrates into the host cell genome. Ribonucleic acid, a molecule similar to DNA, whose functions include decoding the instructions for protein synthesis that are carried by the genes; comprises the genetic material of some viruses. Sharp laboratory items such as syringe needles, scalpel and razor blades, and broken glass. The production of a large random collection of cloned fragments of the DNA of an organism, from which genes of interest can later be selected. Appendix - Page 58
Page 1 and 2:
THE SINGAPORE BIOSAFETY GUIDELINES
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APPENDICES Appendix 1 Other Relevan
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1.4 DEFINITIONS OF ABBREVIATIONS Fo
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2.2 EXEMPTIONS 2.2.1 The following
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SECTION 3: SUMMARY OF PROCEDURES 3.
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3.3 CATEGORY A - EXPERIMENTS REQUIR
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SECTION 4: EXPERIMENTS COVERED BY T
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4.2 CATEGORY B - EXPERIMENTS REQUIR
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SECTION 5: ROLES AND RESPONSIBILITI
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5.2.5 Monitoring of Work The IBC sh
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• submit a new proposal form to t
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• All materials that are genetica
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6.5 SUPPLY OF GENETICALLY MANIPULAT
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6.6.5 The form should contain any r
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BLANK PAGE
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3. Worldwide guidelines specific to
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xiv. AS 2252.2: 1994: xv. AS 2647:
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APPENDIX 2: LIST OF GMAC-APPROVED H
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APPENDIX 3: INSTRUCTIONS FOR COMPLE
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PROPOSAL FORM FOR ASSESSMENT OF GEN
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-----------------------------------
Page 43 and 44: ) Describe the techniques to be emp
Page 45 and 46: ii) Category B iii) Category C C. R
Page 47 and 48: APPENDIX 4: TOXINS For work involvi
Page 49 and 50: 4. Procedures for handling viruses
Page 51 and 52: staff shall handle these infected a
Page 53 and 54: APPENDIX 7: REQUIREMENTS FOR GENETI
Page 55 and 56: equire the provision of a front cov
Page 57 and 58: Signs 10. An automatically-starting
Page 59 and 60: 9. All plants in the plant house sh
Page 61 and 62: APPENDIX 12: PROCEDURES FOR EXPERIM
Page 63 and 64: APPENDIX 13: DESIGN OF ANIMAL FACIL
Page 65 and 66: Other Work Being Performed in the S
Page 67 and 68: permitted in animal rooms. Personne
Page 69 and 70: APPENDIX 15: REQUIREMENTS FOR GENET
Page 71 and 72: APPENDIX 16: REQUIREMENTS FOR INSEC
Page 73 and 74: APPENDIX 17: REQUIREMENTS FOR INSEC
Page 75 and 76: APPENDIX 18: PROCEDURES FOR EXPERIM
Page 77 and 78: From Bio-Security Assurance Arrange
Page 79 and 80: VIRUSES (con’t) Family and genus
Page 81 and 82: PESTS (cont’d) INSECTS MAIN HOST
Page 83 and 84: PESTS (cont’d) NEMATODES MAIN HOS
Page 85 and 86: PESTS (cont’d) NEMATODES MAIN HOS
Page 87 and 88: From the Notifiable Diseases List o
Page 89 and 90: From the Notifiable Diseases List o
Page 91 and 92: APPENDIX 20: GLOSSARY Note: Words a
Page 93: fusion gamete gene gene therapy gen
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