Biosafety Guidelines For Research On Genetically Modified ...

containment
containment level
defective virus
deliberate release
DNA
donor
Drosophila
embryo-rescue
Escherichia coli (E. coli)
Escherichia coli K12
Escherichia coli B
eukaryotic
expression
Prevention of the spread of genetically manipulated
organisms outside the laboratory. Physical containment is
accomplished by the use of special procedures and
facilities. Biological containment is accomplished by the
use of particular strains of the organism which have a
reduced ability to survive or reproduce in the open
environment.
The degree of physical containment provided by a
laboratory or facility, which depends on the design of the
facility, the equipment installed, and the procedures used.
GMAC physical containment levels are numbered from 2
to 4, 4 being the highest level.
A virus that is unable to reproduce in its host without the
presence of another (‘helper’) virus.
Intentional release of a genetically modified organism into
the open environment.
Deoxyribonucleic acid, the molecule which carries the
genetic information for most organisms; consists of four
bases and a sugar-phosphate backbone.
The organism or cell from which DNA is derived for
insertion into another organism (the host).
A genus of flies whose genetics has been extensively
studied.
The process in plant breeding whereby tissue from young
embryo plants is excised and propagated in vitro for
subsequent growth as differentiated plants.
A bacterium that inhabits the intestinal tract of humans
(and other animals).
A strain of E. coli that has been maintained in culture in
laboratories for many years. It has lost the ability to
colonise the intestinal tract of humans and animals, is
well-characterised genetically, and is often used for
molecular cloning work.
Another well-characterised laboratory strain of E. coli.
Belonging to the group of organisms whose cells contain a
true nucleus. Eukaryotic organisms include animals, plants
and fungi.
Manifestation of a characteristic that is specified by a
gene; often used to mean the production of a protein by a
gene that has been inserted into a host organism.
fungi Non-photosynthetic eukaryotic organisms, including
moulds, that feed on organic matter.
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