Food & Nutrition

Arab Journal of Food & Nutrition
82. Occurrence of Enterobacter sakazakii in Milk Powders, Powdered
Infant Formula and Food Production Environment (2005)
Wail Ahmad Al-Omari\ Jordan University of Science & Technology
Supervisor:Dr. Reyad Shaker
Co-Supervisor: Dr. Mahmood Al-Zu’bi
Enterohacter sakazakii a pathogen associated with powdered infant formula, and
other food products can cause serious infections in neonates. Thirteen commercial
samples of powdered infant formula, 7 commercial samples of milk powder and 10
cereal products were surveyed for the presence of E. sakazakii. In addition, forty four
environmental samples, dry swaps, and food ingredients were drawn from food
factories and tested for the presence of E. sakazakii.
The presence of E. sakazakii was detected and isolated using the current FDA
method of detection using Enterobacteriaceae enrichment broth (EE broth) and violet
red bile glucose agar (VRBG). VRBG presumptive colonies were grown for pigment
production on trypton soya agar (TSA) for 24-72 hours followed by biochemical
profile determination using oxoid API 20E test. Isolates of E. sakazakii were
confirmed by plating them on nutrient agar, supplemented with 4-methylumbelliferyl
α-D-glucoside (α-Mug), followed by viewing colonies under UV for the presence of
fluorescence. Fluorescent E. sakazakii were isolated from powdered infant formula at
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