Book of Abstracts - Ruhr-Universität Bochum
Book of Abstracts - Ruhr-Universität Bochum
Book of Abstracts - Ruhr-Universität Bochum
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P-75<br />
ISBOMC `10 5.7 – 9.7. 2010 <strong>Ruhr</strong>-<strong>Universität</strong> <strong>Bochum</strong><br />
Raman Microscopic Cell Uptake Studies <strong>of</strong><br />
Bioactive Organometal Peptide Conjugates<br />
Thomas Sowik a , Eugen Edengeiser b , Martina Havenith-Newen, b and Ulrich Schatzschneider a *<br />
a Lehrstuhl für Anorganische Chemie I – Bioanorganische Chemie, <strong>Ruhr</strong>-<strong>Universität</strong> <strong>Bochum</strong>,<br />
<strong>Universität</strong>sstr. 150, D-44801 <strong>Bochum</strong>, Germany, E-Mail: thomas.sowik@rub.de.<br />
b Lehrstuhl für Physikalische Chemie II, <strong>Ruhr</strong>-<strong>Universität</strong> <strong>Bochum</strong>, <strong>Universität</strong>sstr. 150, D-44801<br />
<strong>Bochum</strong>, Germany.<br />
A major problem is the lack <strong>of</strong> molecular markers which do not influence the intracellular distribution<br />
and biological activity and show signals where constitutions <strong>of</strong> the cell normal do not. Raman<br />
microscopy, however, is a potent technique for cell imaging and drug uptake studies. 1 The C-D<br />
stretching vibration <strong>of</strong> deuterated compounds appears in a spectroscopic window between 2100 and<br />
2350 cm -1 where bio (macro) molecules do not show vibrational signals. Thus, it is possible to<br />
investigate the cell uptake and metabolism <strong>of</strong> these compounds. 2 Peptide bioconjugates with<br />
cyclopentadienylrhenium- or cyclopentadienylmangenese tricarbonyl are known for their biological<br />
activity depending on the peptide sequence. 3 Their analogues with deuterated metal complexes are<br />
promising candidates for Raman microscopy (Fig. 1). An example is sC18 with the sequence<br />
GLRKRLRKFRNKIKEK-NH2 which delivers metal complex bioconjugates to cancer cells.<br />
Rayleigh signal<br />
1650<br />
1907<br />
1942<br />
2020<br />
2352<br />
0 1000 2000 3000 4000<br />
Wavenumber cm -1<br />
3119<br />
Figure 1: Raman spectrum (left) <strong>of</strong> deuterated cyclopentadienylrhenium(I) tricarbonyl carbocylic acid (right). The C-D<br />
stretching vibrations appear at 2352 cm -1 .<br />
Within this work we have prepared deuterated cyclopentadienyl carboxylic acid, which was reacted<br />
with dirhenium decacarbonyl to deuterated cyclopentadienylrhenium(I) tricarbonyl carboxylic acid.<br />
Subsequently, bioconjugates were prepared and their biodistribution and cell-uptake was studied using<br />
Raman spectroscopy and microscopy.<br />
Reference<br />
1. C. Matthäus, T. Chernenkoa, L. Quinteroc, L. Milanb, A. Kaleb, M. Amijib, V. Torchilinb, M.<br />
Diema, Proc. SPIE 2008, 6991.<br />
2. H.-J. v. Manen, A. Lenferink, C. Otto, Anal. Chem. 2008, 80, 9576–9582.<br />
3. K. Meister, J. Niesel, U. Schatzschneider, N. Metzler-Nolte, D. A. Schmidt, M. Havenith, Angew.<br />
Chem. 2010, accepted for publication.<br />
133<br />
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