Book of Abstracts - Ruhr-Universität Bochum

P-33
ISBOMC `10 5.7 – 9.7. 2010 Ruhr-Universität Bochum
The Research of the Interaction between Quercetin and Zirconium (IV) in
Water-Ethanol Medium
Olessya Loiko, a A.Khalitova, a B.Tuleuov, b A.Mashentseva *c
a Karaganda State University, Faculty of Chemistry, Department of Chemistry, Universitetskaya 28,
100000, Karaganda, Kazakhstan. b ISPH “Phytochemistry”,Gasalieva str.4 , 100000, Karaganda,
Kazakhstan. c L.N.Gumilev Eurasian national university, Faculty of Chemistry, Department of
Chemistry, Munajtpasova 5, 010008, Astana, Kazakhstan.
E-mail: olessya0905@gmail.com
It’s known, that the search of new compounds with radioprotective, antioxidant and hepatoprotective
activities is carried out among flavonoids; 1 because of their structural molecule uniqueness they can
hamper not only oxidising processes, but also make transfer of energy and migration of elementary
particles at the irradiation. At the same time, examples of individual flavonoids (rutin and quercetin)
and their derivatives’ usage in the medical practice are single, despite their wide variety, accessibility
of their production sources and relative availability.
It is suggested that the biological activity of an organic ligand can be increased when co-coordinated
or mixed with suitable metal ion, because of its ability to act as a free radical acceptor. 2 From the
pharmacology point of view zirconium (IV) complexes are noted for their significant biological
properties, namely antibacterial and antifungal activities. Information about mechanism of interaction
between zirconium (IV) and quercetin will help to explain and predict biochemical activity of these
components’ mixture in the medical product.
The ability to form complex between quercetin and zirconium (IV) was studied in this work using
spectrophotometer method. Quercetin zirconium (IV) complex is characterized by 3 absorption bands:
in UV and visible parts of spectrum at 360 nm, 395 nm and 475 nm; the extension coefficients (ε)
were calculated for these bands: 1042, 1045 and 2966 correspondingly. In the presence of metal ions,
a bathochromic shift on 55 nm is observed in the absorption spectra of complex. Such bathochromic
shift can be explained by the interaction of cobalt chloride with the free C3-oxo group of quercetin.
Investigated complex was synthesized in the solid state. IR spectra of ligand and complex present
evidence of the coordination between zirconium ions and oxo-group of quercetin. It can be noted that
Zr-O frequencies appear at 559.78, 538.53 and 472.02cm -1 .
As the result of made researches of complex formation between zirconium (IV) and quercetin, the
influence of time, solvent and organic reagent concentration were studied. Studying the complex
solubility, it was determined that the compound sufficiently dissolves in water-ethanol mixture. It has
been shown, that if the content of ethanol is under 15%, the complex will fall in a precipitation. Using
Jobs method it was established, that made compound has content, which formula is C30H18O15Zr. The
stability constant of complex is (9,56 ± 0,01)·10 9 , this number shows that the complex is averagely
stable. Complex forms at the room temperature.
According to the PASS biocsreening calculation follow activities for the synthesized complex are
antiparkinsonian, nootropic, cardioprotectant and creatine kinase inhibitor.
References
1. I.S. Vasil’eva, V.A. Paseshnichenko, The achievements of Biological Chemistry, 2000, 3, 153-156.
2. M.Y. Morandi, J. Pourahmad, Free Radic. Biol. Med. 2003, 34, 243‐250.
91