Book of Abstracts - Ruhr-Universität Bochum

More documents

Recommendations

Info

OP-37 ISBOMC `10 5.7 – 9.7. 2010 Ruhr-Universität Bochum Fluorescent Conjugates Between Dinuclear Rhenium(I) Complexes and Peptide Nucleic Acids (PNA) for Cell imaging and DNA Targeting Emanuela Licandro, *a S. Maiorana, a C. Baldoli, b G. Prencipe, a E. Ferri, a D. Donghi, c M. Panigati, c G. D’Alfonso, c and L. D’Alfonso d a Dipartimento di Chimica Organica e Industriale, Università degli Studi di Milano, Via Venezian 21, I-20133, Milano, Italy. b Istituto di Scienze e Tecnologie Molecolari, C.N.R., Via C. Golgi 19, I-20133 Milano, Italy. c Dipartimento di Chimica Inorganica, Metallorganica e Analitica, Università degli Studi di Milano, Via Venezian 21, I- 20133, Milano, Italy. d Dipartimento di Fisica, Università di Milano-Bicocca, P.za Scienze 6, I-20126 Milan. Italyo. E-mail: emanuela.licandro@unimi.it Peptide nucleic acids (PNA) are structural analogues of DNA, with pseudo-peptide backbone based on N-(2-aminoethyl)glycine, which show high binding affinity and specificity for the complementary DNA and RNA. 1 The conjugation of organometallic complexes to biomolecules finds applications both in diagnostic and therapeutic fields. The incorporation of Re(I) complexes into PNA, can offer a double advantage, due both to its radiochemical and photo-emitting properties. In this communication we describe the set up of the synthesis of new PNA-rhenium organometallic bioconjugates as luminescent compounds for DNA targeting. In particular, we utilized two novel rhenium complexes, namely [Re2(CO)6(�-Cl)2(�-4-COOH-pyridazine)] and Re2(CO)6(�-Cl)2(�-4- (CH2)3COOH-pyridazine)], belonging to a recently developed family of dimeric luminescent rhenium(I) complexes, 2 which were conjugated with the tymine PNA monomer and decamer. The second complex was prepared in order to check the influence of the n-propyl chain spacer on the lifetime and quantum yields of the emission of the PNA-rhenium bioconjugate. The most fluorescent Re-PNA conjugate also showed two photon absorption properties as assessed by “in cell” experiments, that revealed that it permeates the cell membrane, staining both the cytoplasm and the nucleus. Although more detailed experiments are needed, in order to establish the kinetics of the process and to set a lower limit to the sample concentration to be used, these preliminary results indicate that the Re- PNA conjugate is viable as a fluorophore for cell imaging. References 1. Peptide Nucleic Acids; 2nd Ed.; P. E. Nielsen, Ed.; Horizon Bioscience: Norfolk, UK, 2004. 2. D. Donghi, G. D’Alfonso, M. Mauro, M. Panigati, P. Mercandelli, A. Sironi, P. Mussini, L. D’Alfonso, Inorg. Chem. 2008, 47, 4243-4255. 53
OP-38 ISBOMC `10 5.7 – 9.7. 2010 Ruhr-Universität Bochum Design of Cyclometalated Iridium(III) Polypyridine Complexes as Luminescent Biological Labels and Probes Kenneth Kam-Wing Lo Department of Biology and Chemistry, City University of Hong Kong, Tat Chee Avenue, Kowloon, Hong Kong, P. R. China. E-mail: bhkenlo@cityu.edu.hk Many cyclometalated iridium(III) polypyridine complexes exhibit intense and long-lived emission that is very sensitive to the molecular structures and local environments of the complexes. These interesting properties allow the complexes to serve as useful probes for various biological molecules including oligonucleotides, peptides, and proteins. We have attached amine- and sulfhydryl-specific reactive functional groups such as isothiocyanate, aldehyde, and iodoacetamide to cyclometalated iridium(III) polypyridine complexes of the type [Ir(N^C)2(N^N)] + to yield new luminescent labels for biomolecules. Additionally, we have designed related iridium(III) polypyridine complexes appended with various biological substrates including indole, �-estradiol, biotin, and lipids, and utilized the complexes as luminescent probes for indole-binding proteins, estrogen receptors, avidin, and lipidbinding proteins, respectively. Some of these complexes show interesting dual-emissive properties that enable the biological binding event to be reflected by a change of emission profiles of the probes. Furthermore, we have recently developed DNA-metallointercalators, dendrimers, and PEGylation reagents derived from luminescent iridium(III) polypyridine complexes. We have focused on the molecular design, photophysical properties, biomolecule-binding behavior, cytotoxicity, and cellularuptake characteristics of these luminescent probes. R 1 R 1 References C C N Ir N N N R2 + Emission Intensity (A. U.) 500 550 600 650 700 750 Wavelength / nm 1. K. K.-W. Lo, K. Y. Zhang, C.-K. Chung, K. Y. Kwok, Chem. Eur. J. 2007, 13, 7110 – 7130. 2. K. K.-W. Lo, P.-K. Lee, J. S.-Y. Lau, Organometallics 2008, 27, 2998 – 3006. 3. K. K.-W. Lo, K. Y. Zhang, S.-K. Leung, M.-C. Tang, Angew. Chem. Int. Ed. 2008, 47, 2213 – 2216. 4. J. S.-Y. Lau, P.-K. Lee, K. H.-K. Tsang, C. H.-C. Ng, Y.-W. Lam, S.-H. Cheng, K. K.-W. Lo, Inorg. Chem. 2009, 48, 708 – 719. 5. K. Y. Zhang, S. P.-Y. Li, N. Zhu, I. W.-S. Or, M. S.-H. Cheung, Y.-W. Lam, K. K.-W. Lo, Inorg. Chem. 2010, 49, 2530 – 2540. 54 + ER�
Page 1 and 2:
ISBOMC ‘10 5th International Symp
Page 3 and 4: Foreword ISBOMC `10 5.7 - 9.7. 2010
Page 5 and 6: ISBOMC `10 5.7 - 9.7. 2010 Ruhr-Uni
Page 7 and 8: General information ISBOMC `10 5.7
Page 9 and 10: Monday, July 5 th 2010 16:00 to 18:
Page 19 and 20: OP-2 ISBOMC `10 5.7 - 9.7. 2010 Ruh
Page 23 and 24: OP-6 ISBOMC `10 5.7 - 9.7 Ruhr-Univ
Page 27 and 28: OP-10 ISBOMC `10 5.7 - 9.7. 2010 Ru
Page 33 and 34: OP-16 Ru N Cl Cl N Ru Cl Cl ISBOMC
Page 53: OP-36 ISBOMC `10 5.7 - 9.7. 2010 Ru
Page 61 and 62: P-02 ISBOMC `10 5.7 - 9.7. 2010 Ruh
Page 105 and 106:
P-46 ISBOMC `10 5.7 - 9.7. 2010 Ruh
Page 107 and 108:
P-48 ISBOMC `10 5.7 - 9.7. 2010 Ruh
Page 109 and 110:
P-50 ISBOMC `10 5.7 - 9.7. 2010 Ruh
Page 111 and 112:
P-52 ISBOMC `10 5.7 - 9.7. 2010 Ruh
Page 113 and 114:
P-54 ISBOMC `10 5.7 - 9.7. 2010 Ruh
Page 115 and 116:
P-56 ISBOMC `10 5.7 - 9.7. 2010 Ruh
Page 117 and 118:
P-58 ISBOMC `10 5.7 - 9.7. 2010 Ruh
Page 119 and 120:
P-60 ISBOMC `10 5.7 - 9.7. 2010 Ruh
Page 121 and 122:
P-62 ISBOMC `10 5.7 - 9.7. 2010 Ruh
Page 123 and 124:
P-64 ISBOMC `10 5.7 - 9.7. 2010 Ruh
Page 125 and 126:
P-66 ISBOMC `10 5.7 - 9.7. 2010 Ruh
Page 127 and 128:
P-68 ISBOMC `10 5.7 - 9.7. 2010 Ruh
Page 129 and 130:
P-70 ISBOMC `10 5.7 - 9.7. 2010 Ruh
Page 131 and 132:
P-72 ISBOMC `10 5.7 - 9.7. 2010 Ruh
Page 133 and 134:
P-74 ISBOMC `10 5.7 - 9.7. 2010 Ruh
Page 135 and 136:
P-76 ISBOMC `10 5.7 - 9.7. 2010 Ruh
Page 137 and 138:
P-78 ISBOMC `10 5.7 - 9.7. 2010 Ruh
Page 139 and 140:
P-80 ISBOMC `10 5.7 - 9.7. 2010 Ruh
Page 141 and 142:
P-82 ISBOMC `10 5.7 - 9.7. 2010 Ruh
Page 143 and 144:
P-84 ISBOMC `10 5.7 - 9.7. 2010 Ruh
Page 145 and 146:
Sergey Abramkin Universität Wien,
Page 147 and 148:
Tensim Dallagi ENSCP, France t.dall
Page 149 and 150:
Prof. Dr. Toshikazu Hirao Osaka Uni
Page 151 and 152:
ISBOMC `10 5.7 - 9.7. 2010 Ruhr-Uni
Page 153 and 154:
Obiora Augustine Orakwue Hyqid Nige
Page 155 and 156:
ISBOMC `10 5.7 - 9.7. 2010 Ruhr-Uni
Page 157:
ISBOMC `10 5.7 - 9.7. 2010 Ruhr-Uni
show all

Book of Abstracts - Ruhr-Universität Bochum

Create successful ePaper yourself

Delete template?

Save as template?