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Table 1. Molecular properties of Zwt and ZminPeptide Number of Helices Approximated Size K DZ wt 3 7kD 3nMZ min 2 4kD 15nMWhen covalently immobilized onto a commercially available solid phase coupling gel(Sulfo Link® Pierce), Z min was able to efficiently capture hIgG (data not shown). Covalent,site-specific immobilization was conveniently generated through the free thiol group on asingle cysteine residue in the peptide. Elution of the bound protein was straightforward anda concentrated hIgG sample was easily obtained. These results show that the backbonecyclized2-helix domain Z min can be employed for affinity purification of immunoglobulins,demonstrating a potential application for the minimized protein.AcknowledgmentsThis study was supported by a grant in the SAMBIO program from VINNOVA.References1. Starovasnik, M.A., Braisted, A.C., Wells, J.A. Proc. Natl. Acad. Sci. U.S.A. 94, 10080-10085(1997).2. Blanco-Canosa, J.B., Dawson, P.E. Angew. Chem. Int. Ed. Engl. 47, 6851-6855 (2008).119