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Broad NH signals spread within the region of 1.2 ppm could not be applied for theassignment based on COSY/TOCSY spectra.BiologyThe synthesized compounds were tested for their potential effects on mitogen - induced T-and B-cell proliferation in the mouse model with cyclosporine A (CsA) as a reference drug.12001000AFC/10 6 cells800600400200**************0DMSO CsA 6R 7RFig.1. Effects of the peptides on the secondary, humoral immune response of mousesplenocytes to SRBC in vitro.The results revealed strong, dose dependent inhibitory effects of both 6R and 7Rcompounds on the proliferation of splenocytes which were more pronounced in the case of6R and comparable at 10 and 100 µg/mL concentrations to these of CsA (data not shown).The inhibitory actions of the 6R and 7R compounds on PWM-induced cell proliferationfollowed the same pattern as described above for ConA-induced proliferation. Thecompounds were tested for their ability to suppress the humoral immune response in vitroat 1-100 µg/mL concentrations. The results shown in Figure 1 demonstrated that bothpeptides strongly inhibited the number of antibody-forming cells at concentrations of 10and 100 µg/mL and these effects were comparable to the inhibitory action of CsA at thisconcentration range.Activity of the 6S and 7S in comparison to 6R and 7R was smaller in similar tests(data not shown). Interestingly, the cytotoxic effect of all peptides with regard tomononuclear cells from human blood was comparable to that of CLA. The statisticallysignificant inhibition of cell viability was observed only at 100-25 µg/mL concentration for6R.AcknowledgmentsSupported by Technical University of Lodz, grant DS-I18/12/2010.References1. Tuchscherer, G., Mutter, M. Chimia 55, 306-313 (2001).2. Katarzyńska, J., Jankowski, S., Huben, K., Leplawy, M.T., Zabrocki, J. In Benedetti, E., Pedone, C.(Eds) Peptides 2002 (Proceedings of the 27 th European Peptide Symposium), Italy, Napoli, 2002,p.160-161.3. a) Siemion, I.Z., Pędyczak, A., Strug, I., Wieczorek, Z. Arch. Immunol. Ther. Exp. 42, 459-465(1994); b) Gaymes, T.J., Cebrat, M., Siemion, I.Z., Kay, J.E. FEBS Lett. 418, 224-227 (1997); c)Benedetti, E., Padone, C. J. Pept. Sci. 11, 268-272 (2005); d) Picur, B., Cebrat, M., Zabrocki, J.,Siemion, I.Z. J. Pept. Sci. 12, 569-574 (2006).465