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performed at 25 o C with mixing times of 120 and 200 ms, and number of scans of 96 and216 respectively (Figure 2).The standard Wüthrich method was used for frequency resonance assignment. Thepeptide structure was calculated using CYANA by achieving molecular dynamicsimulation in the torsion angle space on 300 random starting structures which resulted in 20structures with a backbone RMSD of 1.3A o . This analogue has a turn like structure betweenamino acids 4 and 6 (Figure 3).Fig. 2 The NOESY 2D spectra (DMSO-d6, 25 o C,mixing time 200ms).Fig. 3. Stereoview of triptorelin analogue (I).Results indicate that proliferation of human breast and ovarian cancer cell lines is dosedependentlyinhibited. The inhibitory efficiency of this new analogue is proved to be higherthan the original triptorelin. It seems that the existence of a new functional group or theshape of the molecule could increase its biological activity compared to triptorelin. Thedesign and synthesis of some novel analogues and investigation of their anti-cancer activityis in progress in our laboratory.AcknowledgmentsThe authors would like to thank K. N. Toosi University of Technology research office for financialsupport. They also gratefully acknowledgement Kimia Exir for chemical donation and financialsupport.References1. Maudsley, S., Davidson, L., Pawson, A.J., Chan, R., Lopez de Maturana, R. Cancer Research 64,7533-7544 (2004).2. Tourwé, D., Van Hemel, J., Piron, J. In Synthesis of peptides and peptidomimetics; Eds. Felix, A.,Moroder, M., Toniolo, C., Thieme: Stuttgart, 2004, E22C, 606.3. Arabanian, A., Mohammadnejad, M., Balalaie, S., Gross, J.H. Bioorg. Chem. Lett. 19, 887-890(2009).4. Mirzaei Saleh-Abady, M., et al. Biopolymers (Peptide Science) 94 (3), 292-298 (2009).577