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AB0 50 100 150Fig. 3. Yeast cells were incubated with increasing concentrations of SN1 and weredetected for fluorescence. (A) Columns chart describing the penetration level of SN1 overtime to the yeast cells; (B) Fluorescence microscopy images of the penetrated cells.To minimize proteolysis, we have designed the BP units so that their incorporation willform a stable peptomer sequence on solid support, using the “sub-monomer” procedure ofNuss, et al [2]. We used the BP units to synthesize a yeast permeable photo-affinity label,called SN1 (Figure 2). Fluorescein and biotin were incorporated into the structure of SN1,in addition to the BP unit, to enable additional validation techniques. Preliminary resultsindicate that SN1 penetrated into yeasts, as can be seen in Figure 3, and thus can be used tostudy protein-protein interactions in this system.We are now constructing a new analog with an additional functional group that bindsspecifically to a target protein and plan to use it to investigate the role of the targetedprotein in yeast proteomics.References1. Suva, L.J., Flannery, M.S., Caulfield, M.P., Findlay, D.M., Jüppner, H., Goldring, S.R., Rosenblatt,M., Chorev. M. J. Pharmacol. Exp. Ther. 283, 876 (1997).2. Nuss, J.M., Desai, M.C., Zuckermann, R.N., Singh, R., Renhowe, P.A., Goff, D.A., Chinn, J.P.,Wang, L., Dorr, H., Brown, E.G., Subramanian, S. Pure Appl. Chem. 69, 447 (1997).157